Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata

Mouffouk, Chaima; Hambaba, Leila; Haba, Hamada; Mouffouk, Soumia; Bensouici, Chawki; Mouffouk, Sara; Hachemi, Messaoud; Khadraoui, Hachani

doi:10.1007/s13596-018-0320-3

Cited by 24 publications

(30 citation statements)

References 45 publications

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“…Chloroform contained the lowest rate. The results we attained are consistent with those of Mouffouk et al [26] who found that the highest phenolic and flavonoids content were recorded in the ethyl acetate extract, followed by n-butanolic extract. Saeed et al [27] have also noted similar results.…”

Section: Discussionsupporting

confidence: 92%

Anticholinesterase and antioxidant activities of foliar extract from a tropical species: Psidium guajava L. (Myrtaceae) grown in Algeria

Bouchoukh

Hazmoune

Boudelaa

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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Guava (Psidium guajava L.) is a fruit tree largely used in folk medicine in tropical and subtropical areas. This exotic species was introduced in a botanical garden in the northeast of Algeria in the 1950’s. The aim of this study is to estimate, for the first time, the antioxidant and anticholinesterase activities of chloroform, ethyl acetate and n-butanol extracts of P. guajava growing in Algeria. Six antioxidant assays were tested, results showed very important efficiency in free radical scavenging, reducing power and β-carotene bleaching of tested extracts. Values of IC50 or A0.5 of some samples were lower than those of standards. With regard to anticholinesterase activity, the inhibitory of both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was investigated. The extracts exhibited interesting capacity to inhibit these enzymes with low values of IC50 and even less than that of galanthamine. These activities were correlated with total phenolic content which was more important compared to the one found in extracts from trees growing in tropical and subtropical region. This could be due to resistance and adaptation of P. guajava grown in Algeria. The data obtained suggest the use of bioactive compounds from P. guajava leaves as antioxidant and drugs for symptomatic treatment of Alzheimer disease.

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Section: Discussionsupporting

confidence: 92%

Anticholinesterase and antioxidant activities of foliar extract from a tropical species: Psidium guajava L. (Myrtaceae) grown in Algeria

Bouchoukh

Hazmoune

Boudelaa

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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“…The total phenolic content of crude extracts from the plant E. altissima was determined by the Folin-Ciocalteu method (Mouffouk et al, 2018). 200 µL of different samples were added to 1000 µL of diluted Folin-Ciocalteu reagent (10%).…”

Section: Total Phenolic Contentmentioning

confidence: 99%

“…The total flavonoid content was estimated using the trichloroaluminum method (Mouffouk et al, 2018). 1 mL of trichloroaluminum solution prepared in water (AlCl 3 , 2%) was added to 1 mL of the diluted samples.…”

Section: Total Flavonoid Contentmentioning

confidence: 99%

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Quantification of total bioactive contents and evaluation of the antioxidant and antibacterial activities of crude extracts from Ephedra altissima Desf.

Bouafia

Mouffouk

Haba

2021

Acta Sci. Biol. Sci.

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The present work describes the in vitro biological activities of the crude extracts (petroleum ether, ethyl acetate and n-butanol) prepared from the species Ephedra altissima Desf. The estimation of total phenolic, flavonoid and tannin contents were carried out using the Folin-Ciocalteu, trichloroaluminum and acidified vanillin methods, respectively. The evaluation of the in vitro antioxidant activities were performed by three different methods namely: scavenging of the free radical ABTS, permanganate reducing antioxidant capacity, and potentiometric assay. In addition, the antibacterial activity was assessed by the agar disk diffusion assay against seven bacterial strains. The results of the phytochemical screening revealed the presence of several types of secondary metabolites. The EtOAc extract exhibited the highest content of phenols (125.62 ± 1.51 μg EGA mg-1 of extract). The greatest flavonoid and tannin contents were observed for n-BuOH extract (19.18 ± 0.39 µg EQ mg-1 of extract and 8.95 ± 1.70 μg EC mg-1 of extract, respectively). Moreover, the EtOAc extract revealed potent antioxidant activity in all the tested methods. Furthermore, the aqueous extract from the species E. altissima showed a good ability to reduce iron III to iron II with a value of 0.68 ± 0.3 moL eq L-1 in potentiometric assay. All the crude extracts (PE, EtOAc and n-BuOH) displayed inhibition of bacterial growth against at least three strains with values of MIC ranging from 3.125 to 50 µg mL-1. Therefore, these results suggest that Ephedra altissima could be used as an important source of natural bioactive compounds with antioxidant and antibacterial properties.

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“…The total flavonoids content of each extract was determined by a colorimetric method as described by mouffouk et al 24 . 1 mL of fractions was mixed with 1 mL of aluminium chloride (AlCl3) solution (2%) and allowed to stand for 10 min.…”

Section: Total Flavonoids Contentmentioning

confidence: 99%

Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

Hamoudi¹,

Amroun²,

Khennouf³

et al. 2020

J. Drug Delivery Ther.

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Ephedra nebrodensis, is a medicinal plant, traditionally in Algeria used for the cure of various ailments such as hepatic disorders. On this basis in present study the phytochemical screening and in vitro antioxidant activity of various fractions (n-hexane, chloroform, ethyl acetate, n-butanol and water) of ethanol extract obtained by maceration from aerial parts of E. nebrodensis were investigated. Different fractions were accessed for their yield percentage, total polyphenol content (TPC), total flavonoid content (TFC), total tannins content (TTC) and antioxidant potential (DPPH - 2,2-diphenyl-1-picrylhydrazyl; ABTS - 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), reducing power assay (FRAP - ferric reducing antioxidant power activity),and bleaching of β-carotene test . Results showed that ethyl acetate fraction (EF) represents the highest amount of total polyphenols, flavonoids and tannins with 269.669 ± 0.031 µg gallic acid equivalent/mg of DE, 44.507 ± 0.003 µg quercetin equivalents/mg of DE and 228.487 ± 1.362 µg tannic acid equivalents/mg of DE, respectively. In antioxidant assays, ethyl acetate fractions (EF) showed the strongest DPPH and ABTS antioxidant (IC50: 0.009 and 0.004 µg/mL) and FRAP potential (EC50: 0.005 ± 0.000 µg/mL), which significantly correlate to its high content of polyphenolics, flavonoids and tannins. Further, fractions were rich in phenolic compounds. The present results support the possible use of the ethyl acetate fraction from E. nebrodensis in pharmaceutical industries as a natural antioxidant. Keywords: Ephedra nebrodensis, polyphenols, flavonoids, tannins, antioxidant capacity.

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Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata

Cited by 24 publications

References 45 publications

Anticholinesterase and antioxidant activities of foliar extract from a tropical species: Psidium guajava L. (Myrtaceae) grown in Algeria

Anticholinesterase and antioxidant activities of foliar extract from a tropical species: Psidium guajava L. (Myrtaceae) grown in Algeria

Quantification of total bioactive contents and evaluation of the antioxidant and antibacterial activities of crude extracts from Ephedra altissima Desf.

Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

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