Acute toxicity and cytogenotoxicity of yangambin isolated from Ocotea duckei vattimo-gil

Castro, M. I.; Lucena, Marta Gerusa Soares de; Silva, Camilla Vila Nova Soares; Xavier, Yhasminie Karine da Silva; Barbosa-Filho, José Maria; Teles, Maria Madalena Rocha Silva; Peixoto, Laisla Rangel; Souza, Ivone Antônia de; Silva, Eliete Cavalcanti da

doi:10.4103/pr.pr_73_20

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…An exsiccata of this species is deposited at the Federal University of Paraíba (AGRA 4309). Extraction and isolation were performed according to Castro et al [23] Gallus gallus domesticus embryos COBB lineage eggs, with 24 hr of fertilization, were kindly provided by G3 Agroavícola, located at Fazenda Santa Teresinha, Riacho das Almas, Pernambuco State, Brazil (8°08'23.0"S 35°51'52.1"W). 120 eggs were used, being separated into three groups, containing 20 eggs each.…”

Section: Yangambinmentioning

confidence: 99%

Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos

Silva¹,

Castro²,

Lucena³

et al. 2021

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Introduction: Yangambin, a lignan predominant in the leaves of Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory and leishmanicidal. Objectives: The aim of this study was to evaluate the embryotoxicity of yangambin on the neurodevelopment of Gallus gallus domesticus embryos. Materials and Methods: 120 fertilized eggs were divided in three groups: G1 (PBS with 0.1 % Tween 80), G2 (50 µg/ml yangambin) and G3 (65 µg/ml yangambin) and each egg was inoculated with 100 µL of the respective solutions. The fertilized eggs were incubated at a temperature of 37.5°C, with a relative humidity of 65% to 75%, for 48 hr and then their embryos were histologically processed. Results: In staging, carried out according to Hamburger and Hamilton (1951), variations of stages were identified. In all groups, the morphological analysis revealed the closure of the anterior neuropore and absence of malformations in the optic vesicles and in the secondary encephalic vesicles. In the caudal region, a standard development of the neural tube was observed, with well-segmented somites and regression of the primitive line. The cross sections showed that the internal structure of the somite's, composed of dermatome, myotome and sclerotome, was preserved. The statistical analysis did not show significant differences between the groups regarding the morphometry of the cephalic and caudal regions of the neural tube. Conclusion: Yangambin did not show embryotoxic effects on the neurodevelopment of Gallus gallus domesticus embryos, under the tested conditions.

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Section: Yangambinmentioning

confidence: 99%

Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos

Silva¹,

Castro²,

Lucena³

et al. 2021

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“…Ocotea duckei Vattimo-Gil, also known as Ocotea fasciculata (Nees) Mez, is a plant rich in lignans, alkaloids and essential oils (Martins et al, 2020). It is popularly known as "Louro-de-cheiro", "Louropimenta" or "Louro-canela" and is widely distributed in the Northeast region of Brazil (Castro et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

alidation of an analytical method by HPLC for the quantification of yangambin from the Ocotea duckei species

Peixoto,

Chaves Junior,

Andrade

et al. 2024

BLACPMA

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Ocotea duckei, known as Louro-de-cheiro, belongs to the Lauraceae family and presents lignoid yangambine (YAN) as the main plant marker. This work aimed to develop and validate an analytical method by high performance liquid chromatography for the quantification of YAN. The sample used was the crude ethanolic extract (CEE) obtained from aerial parts. In the developed method, a C18 column was used. The mobile phase was composed of acetonitrile and water (45:55), whereas the method parameters included mobile phase flow rate at 0.8 mL/min, oven temperature at 40°C, and monitoring at 205 nm. In the validation, the parameters of selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification were evaluated. As a result, the developed method is in accordance with the guidelines for validation of analytical methods and presented satisfactory chromatographic parameters for YAN determination. Thus, the present analytical methodology can be applied in the quality control of O. duckei raw materials.

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Acute toxicity and cytogenotoxicity of yangambin isolated from Ocotea duckei vattimo-gil

Cited by 2 publications

References 21 publications

Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos

Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos

alidation of an analytical method by HPLC for the quantification of yangambin from the Ocotea duckei species

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