Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus

Abstract

PurposeThe purpose of this study is to explore the dynamic change of brain-derived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.Materials and MethodsBy using forced-swimming in 4℃ cold ice water and 25℃ warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.ResultsThe short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.ConclusionThe results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

Anxiolytic effects of muscarinic acetylcholine receptors agonist oxotremorine in chronically stressed rats and related changes in BDNF and FGF2 levels in the hippocampus and prefrontal cortex

“…Direct infusion of BDNF leads to recovery of behavioral deficits in depressed rats (Sairanen et al 2005;Siuciak et al 1997) and chronic administration of antidepressants increases BDNF levels in cortical and hippocampal areas (Nibuya et al 1995;Russo-Neustadt et al 2001). Conversely, long-term stress reduces hippocampal BDNF, which, by contributing to the atrophy and even the death of pyramidal neurons in the hippocampus, may play a role in stress-related psychiatric illnesses (Bennett and Lagopoulos 2014;Monteggia et al 2007;Shi et al 2010;Smith et al 1995). In human, a post-mortem study of depressed patients reported a reduction in BDNF expression (Karege et al 2005) and polymorphisms of BDNF gene were associated with increased susceptibility to depression (Neves-Pereira et al 2002;Sen et al 2003).…”

Nerve Growth Factor(s) Mediated Hypothalamic Pituitary Axis Activation Model in Stress Induced Genesis of Psychiatric Disorders

“…Hippocampal pyramidal neurons secrete NGFs in response to stress which correspond to HPA axis activity (123,124), and also TrkB, the receptor for BDNF is found to be co-localized with glucocorticoid receptor (GR) and having mutual influence on each other's signalling in these neurons (122). A study by Lambert et al has shown in primary rat cortical neurons that BDNF induces structural change at GR gene (phosphorylation at serine 155 and 287) and causes significant change in its transcriptome (125).…”

THE EFFECTS OF ETHANOL EXTRACTS OF Centella asiatica LEAF ON SERIAL SERUM BRAIN DERIVED NEUROTROPHIN FACTOR (BDNF) CONCENTRATION OF RATS (SPRAGUE DAWLEY) FOLLOWING CHRONIC STRESS

“…BDNF/TrkB signal transduction pathways may also participate in the process of learning and memory during chronic stress (Li et al, 2007). A study indicates that chronic mild repeated stress results in decrease of BDNF mRNA and protein (Shi et al, 2010). Therefore, we have designed an investigation to study the neuroprotective effect of herbaceous cognitive enhancer, Centella asiatica, on serum BDNF concentration, that is believed to play an important role in learning and memory process, in chronic stressed-rats.…”