Acute Strenuous Exercise Induces an Imbalance on Histone H4 Acetylation/Histone Deacetylase 2 and Increases the Proinflammatory Profile of PBMC of Obese Individuals

Dorneles, Gilson Pires; Boeira, Maria Carolina R.; Schipper, Lucas de Lima; Silva, Ivy R. V.; Elsner, Viviane Rostirola; Lago, Pedro Dal; Peres, Alessandra; Romão, Pedro Roosevelt Torres

doi:10.1155/2017/1530230

Cited by 40 publications

(30 citation statements)

References 64 publications

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“…PBMC of lean and obese individuals were isolated from the peripheral blood using Histopaque 1077 (Sigma‐Aldrich) as described before . Plasma was collected and frozen at −20°C for cytokines analyzes as described below.…”

Section: Methodsmentioning

confidence: 99%

“…Obesity causes low‐grade chronic inflammation, due to the pathological expansion of the adipose tissue and immunometabolic disorders . Increased plasma levels of tumor necrosis alpha (TNF‐α) and interleukin‐6 (IL‐6) are related to the higher frequencies of intermediate (CD14 + CD16+) and non‐classical (CD14‐CD16+) monocytes, which contribute to insulin resistance in obese individuals . Obesity induces an early accumulation of exhausted memory T cells and T helper type 1 (Th1) cells in peripheral blood and reduces the frequency of regulatory T (Treg) cells, whose main function is to maintain self‐tolerance and immune homeostasis The phenotype of Treg cells is defined by the intracellular expression of transcription factor Foxp3 (Forkhead box protein P3), a master regulator for their development and function, and high expression of IL‐2 receptor‐α chain (CD25) .…”

Section: Introductionmentioning

confidence: 99%

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Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men

Dorneles

Silva

Boeira

et al. 2019

Scandinavian Med Sci Sports

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Funding informationWe are grateful to the Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) -Finance Code 001, CAPES (CAPES-PNPD -23038007200/11-08) and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) for financial support. PDL, AP, and PRTR also thank CNPq for fellowships.This study investigated the peripheral frequency of monocytes, CD4 + T cell subsets and the systemic levels of cytokines in lean and obese men with different levels of cardiorespiratory fitness (CRF). Mononuclear cells were obtained from 45 lean and 45 obese men who were assigned into six groups according to their body mass index and CRF (low, moderate, or high VO 2Peak ) to analyze the frequency of monocyte subsets and subpopulations of CD4 + T cells (Treg cells, CD4 + CD25 high CD127 low ; mTeff, CD4 + CD25-CD39+; mTreg, CD4 + CD25+CD39+). The systemic levels of interleukin (IL)-6, IL-10, IL-17a, IL-33, leptin, and tumor necrosis factor-alpha (TNF-α) were also evaluated. Seven sedentary obese men performed one week of high-intensity interval training (HIIT, 3 sessions/week), and blood samples were collected before and 24 hours after the last session for phenotypic analysis of T cells and monocytes. Obese individuals presented an inflammatory profile characterized by lower frequencies of Treg and mTreg cells and higher proportions of proinflammatory monocytes. However, higher CRF status increased the frequencies of Treg cells and mTreg cells and decreased the percentage of CD4 + mTeff cells and intermediate and non-classical monocytes in the peripheral blood from lean and obese men. Systemic lower levels of proinflammatory (IL-6 and TNF-) cytokines and higher concentrations of IL-10 and IL-33 were observed in moderate and higher CRF in all subjects. HIIT increased the proportions of circulating mTreg and Treg cells in sedentary obese individuals. The immunoregulatory role of CRF contributes to the maintenance of low levels of inflammatory mediators. K E Y W O R D S CD39, CD73, exercise, inflammation, monocyte subsets, obesity, T cells, Treg cells, VO 2 peak 1756 | DORNELES Et aL. SUPPORTING INFORMATIONAdditional supporting information may be found online in the Supporting Information section at the end of the article. How to cite this article: Dorneles GP, da Silva I, Boeira MC, et al. Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men. Scand J Med Sci Sports.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men

Dorneles

Silva

Boeira

et al. 2019

Scandinavian Med Sci Sports

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“…Compared to age-matched sedentary controls, master athletes (European Veterans Athletics Championships in 2010, Nyiregyhaza, Hungary) had decreasing ageing-related inflammation in skeletal muscle related to lower levels of miR-7, which has been suggested to be involved in chronic inflammation in the elderly [64]. Additionally, acute strenuous exercise (consisting of stepping up and down from a step until complete exhaustion) led to enhanced chronic low-grade inflammation in PBMCs from obese individuals via an imbalance on Histone H4 Acetylation/Histone Deacetylase 2 as compared to lean subjects [65]. Together, these findings suggest that the impact of physical activity in inflammation is dependent of type, intensity, and clinical settings of exercise interventions [54].…”

Section: Physical Activitymentioning

confidence: 99%

Epigenetic signatures underlying inflammation: an interplay of nutrition, physical activity, metabolic diseases, and environmental factors for personalized nutrition

et al. 2020

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Aim and objective Emerging translational evidence suggests that epigenetic alterations (DNA methylation, miRNA expression, and histone modifications) occur after external stimuli and may contribute to exacerbated inflammation and the risk of suffering several diseases including diabetes, cardiovascular diseases, cancer, and neurological disorders. This review summarizes the current knowledge about the harmful effects of high-fat/high-sugar diets, micronutrient deficiencies (folate, manganese, and carotenoids), obesity and associated complications, bacterial/viral infections, smoking, excessive alcohol consumption, sleep deprivation, chronic stress, air pollution, and chemical exposure on inflammation through epigenetic mechanisms. Additionally, the epigenetic phenomena underlying the anti-inflammatory potential of caloric restriction, n-3 PUFA, Mediterranean diet, vitamin D, zinc, polyphenols (i.e., resveratrol, gallic acid, epicatechin, luteolin, curcumin), and the role of systematic exercise are discussed. Methods Original and review articles encompassing epigenetics and inflammation were screened from major databases (including PubMed, Medline, Science Direct, Scopus, etc.) and analyzed for the writing of the review paper. Conclusion Although caution should be exercised, research on epigenetic mechanisms is contributing to understand pathological processes involving inflammatory responses, the prediction of disease risk based on the epigenotype, as well as the putative design of therapeutic interventions targeting the epigenome.

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“…Epigenetic modification at the TNFA gene locus that modulates the expression of TNF- α mRNA and protein expression results from a concerted and complex network of regulation such as DNA methylation, histone modification, and chromatin remodeling [ 24 , 25 ]. The studies frequently analyzed monocytes and macrophages.…”

Section: Introductionmentioning

confidence: 99%

Granulocyte Colony-Stimulating Factor Alleviates Bacterial-Induced Neuronal Apoptotic Damage in the Neonatal Rat Brain through Epigenetic Histone Modification

Yang

et al. 2018

Oxidative Medicine and Cellular Longevity

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Bacterial meningitis during the perinatal period may cause long-term neurological deficits. The study investigated whether bacterial lipopolysaccharide (LPS) derived from E. coli. led to neuronal apoptosis with an impaired performance of long-term cognitive function involving the activation of histone modification in the TNF-α gene promoter. Further, we looked into the therapeutic efficacy of granulocyte colony-stimulating factor (G-CSF) in a neonatal brain suffering from perinatal bacterial meningitis. We applied the following research techniques: neurobehavioral tasks, confocal laser microscopy, chromatin immunoprecipitation, and Western blotting. At postnatal day 10, the animals were subjected to LPS and/or G-CSF. The target brain tissues were then collected at P17. Some animals (P45) were studied using neurobehavioral tasks. The LPS-injected group revealed significantly increased expression of NF-κB phosphorylation and trimethylated H3K4 in the TNFA gene promoter locus. Furthermore, the caspase-3, neuronal apoptosis expression, and an impaired performance in cognitive functions were also found in our study. Such deleterious outcomes described above were markedly alleviated by G-CSF therapy. This study suggests that selective therapeutic action sites of G-CSF through epigenetic regulation in the TNFA gene promoter locus may exert a potentially beneficial role for the neonatal brain suffering from perinatal bacterial-induced meningitis.

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Acute Strenuous Exercise Induces an Imbalance on Histone H4 Acetylation/Histone Deacetylase 2 and Increases the Proinflammatory Profile of PBMC of Obese Individuals

Cited by 40 publications

References 64 publications

Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men

Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men

Epigenetic signatures underlying inflammation: an interplay of nutrition, physical activity, metabolic diseases, and environmental factors for personalized nutrition

Granulocyte Colony-Stimulating Factor Alleviates Bacterial-Induced Neuronal Apoptotic Damage in the Neonatal Rat Brain through Epigenetic Histone Modification

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