Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala

Tanuma, Masato; Niu, Misaki; Ohkubo, Jin; Ueno, Hiroki; Nakai, Yuka; Yokoyama, Yoshihisa; Seiriki, Kaoru; Hashimoto, Hitoshi; Kasai, Atsushi

doi:10.1186/s13041-022-00987-8

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…Interestingly, the CI and BLA have multiple common upstream areas. Neurons that project to both regions may coordinate their activity in response to a psychological stressor (Tanuma et al., 2022). The hippocampus, as part of the limbic system of the brain, plays an important role in long‐term memory and spatial navigation (Cossart & Khazipov, 2022).…”

Section: Resultsmentioning

confidence: 99%

Whole‐brain mapping of monosynaptic afferent inputs to the CRH neurons in the medial prefrontal cortex of mice

Li,

Zhu

et al. 2023

Journal of Anatomy

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Corticotropin‐releasing hormone (CRH) neurons are densely distributed in the medial prefrontal cortex (mPFC), which plays a crucial role in integrating and processing emotional and cognitive inputs from other brain regions. Therefore, it is important to know the neural afferent patterns of mPFCCRH neurons, which are still unclear. Here, we utilized a rabies virus‐based monosynaptic retrograde tracing system to map the presynaptic afferents of the mPFCCRH neurons throughout the entire brain. The results show that the mPFCCRH neurons receive inputs from three main groups of brain regions: (1) the cortex, primarily the orbital cortex, somatomotor areas, and anterior cingulate cortex; (2) the thalamus, primarily the anteromedial nucleus, mediodorsal thalamic nucleus, and central medial thalamic nucleus; and (3) other brain regions, primarily the basolateral amygdala, hippocampus, and dorsal raphe nucleus. Taken together, our results are valuable for further investigations into the roles of the mPFCCRH neurons in normal and neurological disease states. These investigations can shed light on various aspects such as cognitive processing, emotional modulation, motivation, sociability, and pain.

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Section: Resultsmentioning

confidence: 99%

Whole‐brain mapping of monosynaptic afferent inputs to the CRH neurons in the medial prefrontal cortex of mice

Li,

Zhu

et al. 2023

Journal of Anatomy

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“…The claustrum (CL) has extensive connections with multiple cortical and subcortical brain regions, including the PFC and amygdala (Jackson et al 2018; Smith et al 2020). Although possible CL involvement in social behavior modulation as well as in ethanol effects has not been studied in laboratory rodents, a subpopulation of CL neurons has been shown to modulate stress-induced anxiety-like behavior of male mice through interaction with the BLA (Niu et al 2022; Tanuma et al 2022). However, an important issue of CL involvement in regulation of social interaction and/or anxiety in adolescent rats of both sexes under basal conditions as well as following ethanol challenge still needs to be addressed in future experiments.…”

Section: Discussionmentioning

confidence: 99%

Patterns of neuronal activation following ethanol-induced social facilitation and social inhibition in adolescent cFos-LacZ male and female rats

Towner,

Applegate,

Coleman

et al. 2024

Preprint

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Motives related to the enhancement of the positive effects of alcohol on social activity within sexes are strongly associated with alcohol use disorder and are a major contributor to adolescent alcohol use and heavy drinking. This is particularly concerning given that heightened vulnerability of the developing adolescent brain. Despite this linkage, it is unknown how adolescent non-intoxicated social behavior relates to alcohols effects on social responding, and how the social brain network differs in response within individuals that are socially facilitated or inhibited by alcohol. Sex effects for social facilitation and inhibition during adolescence are conserved in rodents in high and low drinkers, respectively. In the current study we used cFos-LacZ transgenic rats to evaluate behavior and related neural activity in male and female subjects that differed in their social facilitatory or social inhibitory response to ethanol. Subjects were assessed using social interaction on postnatal days 34, 36 and 38 after a 0, 0.5 and 0.75 g/kg ethanol challenge, respectively, with brain tissue being evaluated following the final social interaction. Subjects were binned into those that were socially facilitated or inhibited by ethanol using a tertile split within each sex. Results indicate that both males and females facilitated by ethanol display lower social activity in the absence of ethanol compared to socially inhibited subjects. Analyses of neural activity revealed that females exhibited differences in 54% of examined socially relevant brain regions of interest (ROIs) compared to only 8% in males, with neural activity in females socially inhibited by ethanol generally being lower than facilitated subjects. Analysis of socially relevant ROI neural activity to social behavior differed for select brain regions as a function of sex, with the prefrontal cortex and nucleus accumbens being negatively correlated in males, but positively correlated in females. Females displayed additional positive correlations in other ROIs, and sex differences were noted across the rostro-caudal claustrum axis. Importantly, neural activity largely did not correlate with locomotor activity. Functional network construction of social brain regions revealed further sex dissociable effects, with 90% interconnectivity in males socially inhibited by ethanol compared to 38% of facilitated subjects, whereas interconnectivity in females inhibited by ethanol was 10% compared to nearly 60% in facilitated subjects. However, hub analyses converged on similar brain regions in males and females, with the nucleus accumbens being a hub region in socially inhibited subjects, whereas the central amygdala was disconnected in facilitated subjects. Taken together, these findings support unified brain regions that contribute to social facilitation or inhibition from ethanol despite prominent sex differences in the social brain network.

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“…Pharmacogenetic inhibition of the CLA-Prelimbic cortex circuit during contextual fear conditioning attenuated recent fear retrieval (30). Other studies also showed that stress and footshock could activate the CLA (25,(31)(32)(33). However, the role of the CLA in fear encoding and anxiety disorders remains largely understudied.…”

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confidence: 99%

Neurotoxic lesions of the anterior claustrum influence cued fear memory in rats

Gu,

Dong,

et al. 2024

Front. Psychiatry

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BackgroundThe claustrum (CLA), a subcortical area between the insular cortex and striatum, innervates almost all cortical regions of the mammalian brain. There is growing evidence that CLA participates in many brain functions, including memory, cognition, and stress response. It is proposed that dysfunction or malfunction of the CLA might be the pathology of some brain diseases, including stress-induced depression and anxiety. However, the role of the CLA in fear memory and anxiety disorders remains largely understudied.MethodsWe evaluated the influences of neurotoxic lesions of the CLA using auditory-cued fear memory and anxiety-like behaviors in rats.ResultsWe found that lesions of anterior CLA (aCLA) but not posterior CLA (pCLA) before fear conditioning attenuated fear retrieval, facilitated extinction, and reduced freezing levels during the extinction retention test. Post-learning lesions of aCLA but not pCLA facilitated fear extinction and attenuated freezing behavior during the extinction retention test. Lesions of aCLA or pCLA did not affect anxiety-like behaviors evaluated by the open field test and elevated plus-maze test.ConclusionThese data suggested that aCLA but not pCLA was involved in fear memory and extinction. Future studies are needed to further investigate the anatomical and functional connections of aCLA subareas that are involved in fear conditioning, which will deepen our understanding of CLA functions.

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Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala

Cited by 6 publications

References 32 publications

Whole‐brain mapping of monosynaptic afferent inputs to the CRH neurons in the medial prefrontal cortex of mice

Whole‐brain mapping of monosynaptic afferent inputs to the CRH neurons in the medial prefrontal cortex of mice

Patterns of neuronal activation following ethanol-induced social facilitation and social inhibition in adolescent cFos-LacZ male and female rats

Neurotoxic lesions of the anterior claustrum influence cued fear memory in rats

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