Acute-phase proteins in pregnant Sudanese women with severe Plasmodium falciparum malaria

Saad, Alfarazdeg A.; Mohamed, Omar E; Ali, AbdelAziem A.; Bashir, Ahmed Muhammad; Ali, Nagi I.; Elbashir, Mustafa I.; Adam, Ishag

doi:10.1016/j.trstmh.2012.06.004

Cited by 10 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, iTRAQ has been used as a quantitative proteomic technique to analyze sera from patients infected with malaria ( P. knowlesi , P. falciparum or P. vivax ) and non-infected healthy subjects. The present data has validated the expression of CADM4 and HAP in other studies [ 16 , 17 , 18 ]. The expression levels of CADM4 and CRP were increased two-fold in the sera of malaria patients relative to controls.…”

Section: Resultssupporting

confidence: 90%

Identification of Protein Markers in Patients Infected with Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax

Bee

Lau

et al. 2014

IJMS

View full text Add to dashboard Cite

Malaria is caused by parasitic protozoans of the genus Plasmodium and is one of the most prevalent infectious diseases in tropical and subtropical regions. For this reason, effective and practical diagnostic methods are urgently needed to control the spread of malaria. The aim of the current study was to identify a panel of new malarial markers, which could be used to diagnose patients infected with various Plasmodium species, including P. knowlesi, P. vivax and P. falciparum. Sera from malaria-infected patients were pooled and compared to control sera obtained from healthy individuals using the isobaric tags for relative and absolute quantitation (iTRAQ) technique. Mass spectrometry was used to identify serum proteins and quantify their relative abundance. We found that the levels of several proteins were increased in pooled serum from infected patients, including cell adhesion molecule-4 and C-reactive protein. In contrast, the serum concentration of haptoglobin was reduced in malaria-infected individuals, which we verified by western blot assay. Therefore, these proteins might represent infectious markers of malaria, which could be used to develop novel diagnostic tools for detecting P. knowlesi, P. vivax and P. falciparum. However, these potential malarial markers will need to be validated in a larger population of infected individuals.

show abstract

Section: Resultssupporting

confidence: 90%

Identification of Protein Markers in Patients Infected with Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax

Bee

Lau

et al. 2014

IJMS

View full text Add to dashboard Cite

show abstract

“…These proteins play a key role in the interaction between inflammation and vascular function, and alterations in their levels are associated with an increased risk of poor birth outcomes, including PTB [18,42]. We also show that the inflammatory markers, CRP and sTNFRII, were elevated in MIP, in agreement with previous data [21,43–47]. Tight control of inflammation is required for healthy pregnancies, especially for proper placentation and fetal development, whereas dysregulated inflammation is associated with poor placental function and adverse pregnancy outcomes, including preeclampsia [48–50].…”

Section: Discussionsupporting

confidence: 92%

Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study

et al. 2019

View full text Add to dashboard Cite

BackgroundMalaria in pregnancy is associated with adverse birth outcomes. However, the underlying mechanisms remain poorly understood. Tight regulation of angiogenic, metabolic, and inflammatory pathways are essential for healthy pregnancies. We hypothesized that malaria disrupts these pathways leading to preterm birth (PTB).Methods and findingsWe conducted a secondary analysis of a randomized trial of malaria prevention in pregnancy conducted in Malawi from July 21, 2011, to March 18, 2013. We longitudinally assessed circulating mediators of angiogenic, metabolic, and inflammatory pathways during pregnancy in a cohort of HIV-negative women (n = 1,628), with a median age of 21 years [18, 25], and 562 (35%) were primigravid. Pregnancies were ultrasound dated, and samples were analyzed at 13 to 23 weeks (Visit 1), 28 to 33 weeks (Visit 2), and/or 34 to 36 weeks (Visit 3). Malaria prevalence was high; 70% (n = 1,138) had PCR-positive Plasmodium falciparum infection at least once over the course of pregnancy and/or positive placental histology. The risk of delivering preterm in the entire cohort was 20% (n = 304/1506). Women with malaria before 24 weeks gestation had a higher risk of PTB (24% versus 18%, p = 0.005; adjusted relative risk [aRR] 1.30, 95% confidence interval [CI] 1.04–1.63, p = 0.021); and those who were malaria positive only before week 24 had an even greater risk of PTB (28% versus 17%, p = 0.02; with an aRR of 1.67, 95% CI 1.20–2.30, p = 0.002). Using linear mixed-effects modeling, malaria before 24 weeks gestation was associated with altered kinetics of inflammatory (C-Reactive Protein [CRP], Chitinase 3-like protein-1 [CHI3L1], Interleukin 18 Binding Protein [IL-18BP], soluble Tumor Necrosis Factor receptor II [sTNFRII], soluble Intercellular Adhesion Molecule-1 [sICAM-1]), angiogenic (soluble Endoglin [sEng]), and metabolic mediators (Leptin, Angiopoietin-like 3 [Angptl3]) over the course of pregnancy (χ2 > 13.0, p ≤ 0.001 for each). Limitations include being underpowered to assess the impact on nonviable births, being unable to assess women who had not received any antimalarials, and, because of the exposure to antimalarials in the second trimester, there were limited numbers of malaria infections late in pregnancy.ConclusionsCurrent interventions for the prevention of malaria in pregnancy are initiated at the first antenatal visit, usually in the second trimester. In this study, we found that many women are already malaria-infected by their first visit. Malaria infection before 24 weeks gestation was associated with dysregulation of essential regulators of angiogenesis, metabolism, and inflammation and an increased risk of PTB. Preventing malaria earlier in pregnancy may reduce placental dysfunction and thereby improve birth outcomes in malaria-endemic settings.

show abstract

“…Studies were categorized as those assessing the association between iron supplementation and malaria risk in pregnancy [33]–[39], iron deficiency and malaria risk in pregnancy [34], [40]–[50], iron biomarkers and malaria risk in pregnancy [40]–[43], [46], [47], [50]–[61], or iron treatment and malaria risk in pregnancy [62], [63]. These included two randomized controlled trials [36], [37] and one sub-group analysis [33] from the main trial [37], two prospective cohort studies [38], [62], one before-after study [39], one retrospective cohort study [63], six case-control studies [40], [47], [49], [52]–[54], and 18 cross-sectional studies (table 1–4) [34], [35], [41]–[46], [48], [50], [51], [55]–[61]. One cross-sectional study was described in two articles, the original analyzed serum ferritin relative to malaria infection [57] and the supplemental article considered iron deficiency status controlling for inflammation relative to malaria infection [45].…”

Section: Resultsmentioning

confidence: 99%

The Association between Malaria and Iron Status or Supplementation in Pregnancy: A Systematic Review and Meta-Analysis

et al. 2014

View full text Add to dashboard Cite

IntroductionMalaria prevention and iron supplementation are associated with improved maternal and infant outcomes. However, evidence from studies in children suggests iron may adversely modify the risk of malaria. We reviewed the evidence in pregnancy of the association between malaria and markers of iron status, iron supplementation or parenteral treatment.Methods and FindingsWe searched MEDLINE, EMBASE, the Cochrane Central Register of Controlled Trials, the Global Health Library, and the Malaria in Pregnancy library to identify studies that investigated the association between iron status, iron treatment or supplementation during pregnancy and malaria. Thirty one studies contributed to the analysis; 3 experimental and 28 observational studies. Iron supplementation was not associated with an increased risk of P. falciparum malaria during pregnancy or delivery in Africa (summary Relative Risk = 0.89, 95% Confidence Interval (CI) 0.66–1.20, I2 = 78.8%, 5 studies). One study in Asia reported an increased risk of P. vivax within 30 days of iron supplementation (e.g. adjusted Hazard Ratio = 1.75, 95% CI 1.14–2.70 for 1–15 days), but not after 60 days. Iron deficiency (based on ferritin and C-reactive protein) was associated with lower odds for malaria infection (summary Odds Ratio = 0.35, 0.24–0.51, I2 = 59.2%, 5 studies). With the exception of the acute phase protein ferritin, biomarkers of iron deficiency were generally not associated with malaria infection.ConclusionsIron supplementation was associated with a temporal increase in P vivax, but not with an increased risk of P. falciparum; however, data are insufficient to rule out the potential for an increased risk of P. falciparum. Iron deficiency was associated with a decreased malaria risk in pregnancy only when measured with ferritin. Until there is more evidence, it is prudent to provide iron in combination with malaria prevention during pregnancy.

show abstract

Acute-phase proteins in pregnant Sudanese women with severe Plasmodium falciparum malaria

Cited by 10 publications

References 9 publications

Identification of Protein Markers in Patients Infected with Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax

Identification of Protein Markers in Patients Infected with Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax

Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study

The Association between Malaria and Iron Status or Supplementation in Pregnancy: A Systematic Review and Meta-Analysis

Contact Info

Product

Resources

About