Acute phase proteins altered in the plasma of patients with congenital ventricular septal defect

Zhang, Xi; Wang, Kai; Yang, Qin; Wang, Jun; Xuan, Chao; Liu, Xiaocheng; Liu, Zhigang; He, Guo‐Wei

doi:10.1002/prca.201400166

Cited by 19 publications

(17 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is a large extent due to the major undertaking of sequencing the human and the human genome, which has opened the door for proteomic studies. In our previous studies, we also have screened and identified several serum/plasma biomarkers of cardiovascular disease using proteomic analysis . Many biomarkers have subsequently been identified using proteomic analysis, but few studies have focused on the proteomics of blood in MI.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Screening and Identification of Pregnancy Zone Protein and Leucine‐Rich Alpha‐2‐Glycoprotein as Potential Serum Biomarkers for Early‐Onset Myocardial Infarction using Protein Profile Analysis

Xuan

et al. 2018

Proteomics Clinical Apps

Self Cite

View full text Add to dashboard Cite

Purpose: The present study aims to discover novel serum biomarkers of early-onset myocardial infarction (MI) using proteomic analysis. Experimental design: In the first stage, the iTRAQ-coupled LC-MS/MS technique is utilized to investigate protein profiles of patients with early-onset MI. In the second stage, these candidate proteins are validated using ELISA. Results: A total of 538 proteins are quantified, with pregnancy zone protein (PZP), leucine-rich α-2-glycoprotein (LRG) and Apolipoprotein C-I (Apo C-I) being upregulated and Apolipoprotein A-I (Apo A-I) and Apolipoprotein A-IV (ApoA-IV) downregulated in early-onset MI patients. Results from the validation stage demonstrate that the serum concentrations of PZP and LRG are significantly increased in the early-onset MI group. The correlation between the concentrations of C-reactive protein (CRP) and the two candidate biomarkers is positive. Area under the curve values used to diagnose early-onset MI for LRG and PZP are 0.939 and 0.874, respectively. Conclusions and clinical relevance: Five differential serum proteins are identified in early-onset MI using proteomic analysis. Lipoprotein-related biomarkers further demonstrate the close relationship between lipid metabolism and the disease. Inflammation-associated LRG and PZP may be novel biomarkers of the disease. In addition, changes in these proteins may partly reveal the possible mechanisms in the pathogenesis and pathophysiology of early-onset MI.

show abstract

Section: Discussionmentioning

confidence: 99%

“…As our previous studies have described, serum is the ideal sample for proteome analysis because it is easy to obtain from patients . In addition, an estimated 10 000 different proteins and peptides typically exist in serum, and many of them are secreted by cells as a result of physiologic or pathologic processes .…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of Pregnancy Zone Protein and Leucine‐Rich Alpha‐2‐Glycoprotein as Potential Serum Biomarkers for Early‐Onset Myocardial Infarction using Protein Profile Analysis

Xuan

et al. 2018

Proteomics Clinical Apps

Self Cite

View full text Add to dashboard Cite

show abstract

“…We recently used proteomic methods to demonstrate the plasma protein changes in CHD patients8 that may reveal the possible mechanisms for the prolonged bleeding time in patients with tetralogy of Fallot and the susceptibility to pulmonary infections in patients with CHDs. More recently, we have further reported other protein changes in CHD patients9 with potential clinical implications. We also have for the first time identified alterations of 14 differential proteins or polypeptides in the plasma of patients with various valvular heart diseases, which indicate the possible genetic deficiency in these patients10.…”

mentioning

confidence: 69%

Plasma Proteomic Study in Pulmonary Arterial Hypertension Associated with Congenital Heart Diseases

Zhang

Hou

Wang

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Pulmonary arterial hypertension associated with congenital heart disease (CHD-PAH) has serious consequence and plasma protein profiles in CHD-PAH are unknown. We aimed to reveal the differential plasma proteins in 272 CHD patients with or without PAH. Various types of CHD-PAH were studied. Differential plasma proteins were first detected by iTRAQ proteomic technology and those with significant clinical relevance were selected for further ELISA validation in new cohort of patients. Among the 190 differential plasma proteins detected by iTRAQ, carbamoyl-phosphate synthetase I (CPSI, related to urea cycle and endogenous nitric oxide production) and complement factor H-related protein 2 (CFHR2, related to complement system and coagulant mechanism) were selected for further ELISA validation in new cohort of 152 patients. Both CPSI and CFHR2 were down-regulated with decreased plasma levels (p < 0.01). Thus, we for the first time in CHD-PAH patients identified a large number of differential plasma proteins. The decreased CPSI expression in CHD-PAH patients may reveal a mechanism related to endogenous nitric oxide and the decrease of CFHR2 protein may demonstrate the deficiency of the immune system and coagulation mechanism. The findings may open a new direction for translational medicine in CHD-PAH with regard to the diagnosis and progress of the disease.

show abstract

“…Some limitations of the study should be considered. To detect the proteomic changes, many important but high abundant proteins such as antibodies, apolipoproteins, protease inhibitors and coagulation factors are removed before the proteomic study [ 6 – 8 , 25 ]. On the other hand, some lower concentration- plasma proteins that may be related to the pathology of CAD may not be able to be detected by the present techniques.…”

Section: Discussionmentioning

confidence: 99%

Plasma protein profiling in patients undergoing coronary artery bypass grafting surgery and clinical significance

et al. 2017

Self Cite

View full text Add to dashboard Cite

This study was designed to identify the protein profiling in patients with triple vessel coronary artery disease (CAD) undergoing CABG, in order to detect CAD-related differential proteins in these patients. CABG patients with triple vessel disease with/without left main stenosis (n =160) were compared to normal coronary angiographic subjects (n =160). Plasma samples of 20 males and 20 females in each group were analyzed with iTRAQ technique. ELISA test was used to test the chosen proteins from iTRAQ results in plasma samples from a new cohort of the CABG group (n=120, male/femal=61/59) and control (n =120, male/female=60/60). iTRAQ detected 544 proteins with 35 up-regulated and 41 down-regulated (change fold > 1.2 or < 0.83, p < 0.05). Three proteins including platelet factor 4 (PF4), coagulation factor XIII B chain (F13B), and secreted frizzled-related protein 1 (sFRP1) were selected for validation by using ELISA that demonstrated significant up-regulation of PF4 and sFRP1 (p < 0.05). There was a positive correlation between these proteins and CAD (p < 0.05) and myocardial infarction history (p < 0.05). Thus, we for the first time have found 76 proteins differentially expressed in plasma of CABG patients. The thrombotic disease/inflammation progress-related protein PF4 and sFRP1, a member of the Wnt/fz signal-transduction pathway and related to myocardial repair, are significantly up-regulated in triple-vessel disease with/without left main stenosis. PF4 may be developed as a biomarker for the diagnosis of the severity of CAD requiring CABG procedure.

show abstract

Acute phase proteins altered in the plasma of patients with congenital ventricular septal defect

Cited by 19 publications

References 48 publications

Screening and Identification of Pregnancy Zone Protein and Leucine‐Rich Alpha‐2‐Glycoprotein as Potential Serum Biomarkers for Early‐Onset Myocardial Infarction using Protein Profile Analysis

Screening and Identification of Pregnancy Zone Protein and Leucine‐Rich Alpha‐2‐Glycoprotein as Potential Serum Biomarkers for Early‐Onset Myocardial Infarction using Protein Profile Analysis

Plasma Proteomic Study in Pulmonary Arterial Hypertension Associated with Congenital Heart Diseases

Plasma protein profiling in patients undergoing coronary artery bypass grafting surgery and clinical significance

Contact Info

Product

Resources

About