Acute-phase protein behavior in dairy cattle herd naturally infected with Trypanosoma vivax

Sampaio, Paulo Henrique; Fidélis, Otávio Luiz; Marques, Luiz Carlos; Machado, Rosangela Zacarias; Barnabé, Patrícia de Athayde; André, Marcos Rogério; Balbuena, Tiago Santana; Cadioli, Fabiano Antônio

doi:10.1016/j.vetpar.2015.05.014

Cited by 12 publications

(9 citation statements)

References 41 publications

(57 reference statements)

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“…However, in cattle, the serum levels of α1-antitripsin can be so low that their systemic detection is difficult. Similar findings were described by Fagliari et al (2007) and Sampaio et al (2015).…”

Section: Discussionsupporting

confidence: 91%

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Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender.

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Section: Discussionsupporting

confidence: 91%

“…The serum electrophoretic profile values obtained were similar to those described in the literature for healthy cattle of the same age range (Fagliari et al 2007, Simplício et al 2013, Sampaio et al 2015. The CSF profile has not yet been determined for bovines, so no comparative data are available.…”

Section: Discussionsupporting

confidence: 78%

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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“…ELISA is a widely used technique for seroepidemiological studies for T. vivax in Africa (Dagnachew & Bezie, 2015) and South America (Osório et al, 2008;Sampaio et al, 2015), providing valuable information about herd status.…”

Section: Serological Assaymentioning

confidence: 99%

Follow-up of dairy cattle naturally infected by Trypanosoma vivax after treatment with isometamidium chloride

Neto

Garcia

Fidélis

et al. 2021

Rev. Bras. Parasitol. Vet.

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Trypanosoma vivax infections cause nonspecific clinical signs in cattle associated with aparasitemic intervals, making disease diagnosis a challenge. In Brazil, diminazene aceturate and isometamidium chloride (ISM) are available to treat bovine trypanosomosis. The objective of this study was to follow-up, by molecular and serological techniques, dairy cattle naturally infected by T. vivax after ISM treatment. Thirty cattle naturally infected with T. vivax received two applications of ISM, at a dosage of 1.0 mg/kg intramuscularly, on days 0 and 150. For T. vivax diagnosis, EDTA-blood and serum samples were evaluated on 0, 7, 15, 30, 60, 90, 120, 150, 180, 210, and 240 days after treatment PCR, Loop-mediated isothermal amplification (LAMP) and ELISA. Animals with persistent detection of T. vivax DNA by both PCR and LAMP were found and continuous detection of anti-T. vivax IgG antibodies by ELISA, suggesting the presence of T. vivax resistance to ISM. The combination of LAMP and ELISA tests can prevent misdiagnosis of the parasite clearance in treated cattle, contributing to better disease control. This is the first experiment that demonstrates the persistence infection of T. vivax under ISM treatment in a natural infected herd and evidence of ISM chemotherapy-resistant T. vivax in Brazil.

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“…The plates (Maxisorp ® ; Nunc, Thermo Scientific, Brazil) were incubated in a moist chamber at 37 ºC for 90 minutes. After three washings with PBS-Tween 20 buffer, the positive and negative reference serum and tested serum samples, previously diluted with PBS-Tween 20 solution plus 5% normal Rabbit serum at the following dilutions 1:400 (A. marginale, B. bovis and B. bigemina) and 1:100 (T. vivax) according to Sampaio et al (2015), were added to the ELISA plates. The plates were incubated again at 37 ºC for 90 minutes.…”

Section: Indirect Enzyme-linked Immunosorbent Assay (Ielisa) For Detementioning

confidence: 99%

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Ramos

Mello

Mendes

et al. 2020

Rev. Bras. Parasitol. Vet.

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This study investigated the seropositivity for five different tick-borne agents, namely Anaplasma marginale, Babesia bovis, Babesia bigemina, Coxiella burnetii, Anaplasma phagocytophilum, and Trypanosoma vivax in beef cattle in the Brazilian Pantanal. The serum samples collected from animals (200 cows; 200 calves) were used in indirect enzyme-linked immunosorbent assays (iELISA) to detect IgG antibodies against A. marginale, B. bovis, B. bigemina, and T. vivax, and Indirect Fluorescent Antibody Test (IFAT) for detecting IgG antibodies against C. burnetii and A. phagocytophilum. No correlation was observed between seropositivity for C. burnetii and A. phagocytophilum with other agents whereas moderate correlation was observed for A. marginalexB. bigemina x B. bovis. Cows were more seropositive for T. vivax whereas calves were more seropositive for B. bovis and B. bigemina. The highest number of seropositive animals by a single agent was observed for T. vivax (15.2%). Co-seropositivity for T. vivax + A. marginale was higher in cows (25.5%) and for T. vivax + B. bovis + B. bigemina + A. marginale was higher in calves (57.5%). The high seropositivity correlation for A. marginale x B. bovis x B. bigemina is probably due to the presence of the tick biological vector, Rhipicephalus microplus, in the studied farms. Common transmission pathways, mediated by hematophagous dipterans and fomites, may explain the high co-seropositivity of cows for A. marginale and T. vivax. Low seropositivity to C. burnetii is probably due to the type of breeding system employed (extensive). Seropositivity for A. phagocytophilum in only one animal suggests the occurrence of a cross-serological reaction with another agent of the genus Anaplasma.

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Acute-phase protein behavior in dairy cattle herd naturally infected with Trypanosoma vivax

Cited by 12 publications

References 41 publications

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Follow-up of dairy cattle naturally infected by Trypanosoma vivax after treatment with isometamidium chloride

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

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