Acute milk yield response to frequent milking during early lactation is mediated by genes transiently regulated by milk removal

Wall, Emma; Bond, Jeffery; McFadden, T.B.

doi:10.1152/physiolgenomics.00027.2011

Cited by 10 publications

(18 citation statements)

References 58 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inspired by Fluck (2006), who proposed a very similar model to depict the acute transcriptional response of skeletal muscle to repeated bouts of exercise, it also fits our observations on the mammary gland. It accommodates our observation that the expression of 855 genes was acutely regulated by milk removal, and a discrete subset of those also responded to 4X, but none were uniquely regulated by 4X milking (Wall et al, 2011a). In the model, each milking is associated with an acute transcriptional response, which is represented by the peaks in the diagram.…”

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanissupporting

confidence: 80%

“…Many of these genes were among those previously identified as responsive to IMF of dairy cows (Connor et al, 2008). In addition, we concluded from our previous experiment (Wall et al, 2011a) that some of these genes were acutely regulated by removal of milk from the mammary gland and must mediate the milk yield response to 4X milking. The results of the sequential biopsy experiment support that conclusion, and confirmed genes that we had previously identified as putative responders to 4X milking (Wall et al, 2011a; Wall and McFadden, 2010).…”

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanismentioning

confidence: 82%

“…We then used microarray analysis to identify genes that were differentially expressed in each response. The results of that study revealed that on d 5 of lactation expression of 855 genes was acutely regulated by milk removal but no genes were uniquely associated with the sustained effect of UFM (Wall et al, 2011a). We concluded that a subset of the genes that respond acutely to milking must also regulate the increase in milk yield during IMF.…”

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanismentioning

confidence: 98%

“…We had previously shown that expression of some genes involved in the insulin-like growth factor axis were regulated both by milk removal and by IMF (Wall and McFadden, 2010). Cows were assigned to UFM on d 1 to 21 of lactation and mammary biopsies were obtained from both udder halves on d 5, either immediately after milking only the 4X udder half or 2.5 h after milking both udder halves (Wall et al, 2011a; Wall and McFadden, 2010). We then used microarray analysis to identify genes that were differentially expressed in each response.…”

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanismentioning

confidence: 99%

See 3 more Smart Citations

TRIENNIAL LACTATION SYMPOSIUM: A local affair: How the mammary gland adapts to changes in milking frequency1,2

Wall

McFadden

2012

Journal of Animal Science

Self Cite

View full text Add to dashboard Cite

Regular removal of milk from the mammary gland is critical to maintaining milk secretion. Early studies in rodents demonstrated that changes in milking frequency influenced mammary blood flow, as well as mammary cell number and activity. Later studies in ruminants confirmed those observations and that the response was regulated locally within the mammary gland. In addition, it was discovered that increased milking frequency (IMF) during early lactation stimulated an increase in milk production that partially persisted through late lactation, indicating long-term effects on mammary function. The local mechanisms regulating the mammary response to IMF are poorly understood, although several have been proposed. To gain insight into the mechanisms underlying the mammary response to IMF, and to identify genes associated with the response, we used a functional genomics approach and conducted experiments on dairy cows exposed to unilateral frequent milking (UFM; twice daily milking (2X) of the left udder half, four-times daily milking (4X) of the right udder half). Across multiple experiments, we were unable to detect an effect of UFM on mammary cell proliferation or apoptosis. We have, however, identified distinct transcriptional signatures associated with the mammary response to milk removal and to UFM during early lactation. Sequential sampling of mammary tissue revealed that when UFM was imposed during early lactation, at least two sets of genes were coordinately regulated with changes in differential milk production of 4X vs. 2X udder halves. Moreover, some genes were persistently differentially expressed in 4X vs. 2X udder halves after UFM and were associated with the persistent increase in milk yield. We conclude that a coordinated transcriptional response is associated with the increase in milk yield elicited by IMF during early lactation, and that the two sets of differentially expressed genes may be a marker for the autocrine up-regulation of milk production. Moreover, we propose that we have identified a novel form of imprinting associated with persistent alteration of mammary function, which we term 'lactational imprinting.”

show abstract

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanissupporting

confidence: 80%

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanismentioning

confidence: 82%

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanismentioning

confidence: 98%

Section: Unilateral Frequent Milking: a Powerful Approach To Mechanismentioning

confidence: 99%

See 2 more Smart Citations

TRIENNIAL LACTATION SYMPOSIUM: A local affair: How the mammary gland adapts to changes in milking frequency1,2

Wall

McFadden

2012

Journal of Animal Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…The use of large transcriptomic analysis (i.e., microarray or RNA sequencing) by which to study the adaptation of mammary gland to lactation and/or change in milk production under specific conditions has been carried out in mouse (4,54,122,123,143,189,191,217,236,256), rat (3,47,187), bovine (31,36,56,75,96,144,183,209,218,233,239), sheep (209), goat (71,163,164), human (121,139,147), pig (204), kangaroo (120), and seal (144). It is beyond the scope of the current review to present a thorough discussion of the results from those studies.…”

mentioning

confidence: 99%

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Osorio

Lohakare

Bionaz

2016

Physiological Genomics

182

View full text Add to dashboard Cite

show abstract

Functional genomics: applications to production agriculture.

Buza¹,

McCarthy²

2014

CABI Reviews

View full text Add to dashboard Cite

Functional genomics enables agricultural researchers to investigate how gene expression and regulation contributes to complex production traits at a genome-wide level. There are many different techniques for utilizing functional genomic approaches and new high-throughput technologies are accelerating the amount and complexity of data collected from functional genomics experiments. In this review, we highlight some of the different areas of functional genomics, including some emerging techniques, with a specific focus on how they are being applied to production livestock and aquaculture systems. We look at how transcriptomics, proteomics, metabolomics, interactomics, epigenetics and nutrigenomics are applied to improve our understanding of complex production traits and how the environment affects these traits. We also discuss how changing technologies contribute to functional genomics and the resources agricultural researchers require to ensure that their functional genomics data are effectively translated into benefits for society.

show abstract

Acute milk yield response to frequent milking during early lactation is mediated by genes transiently regulated by milk removal

Abstract: Wall EH, Bond JP, McFadden TB. Acute milk yield response to frequent milking during early lactation is mediated by genes transiently regulated by milk removal.

Cited by 10 publications

References 58 publications

TRIENNIAL LACTATION SYMPOSIUM: A local affair: How the mammary gland adapts to changes in milking frequency1,2

TRIENNIAL LACTATION SYMPOSIUM: A local affair: How the mammary gland adapts to changes in milking frequency1,2

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Functional genomics: applications to production agriculture.

Contact Info

Product

Resources

About