Acute Manipulation and Real-Time Visualization of Membrane Trafficking and Exocytosis in Drosophila

Glashauser, Jade; Camelo, Carolina; Hollmann, Manuel; Sanchez, Jone Isasti; Jacobs, Thea; Backer, Wilko; Förster, Dominique; Luschnig, Stefan

doi:10.2139/ssrn.4065077

Cited by 1 publication

(1 citation statement)

References 47 publications

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“…Additional advantages of researching secretion in Drosophila is the availability of sophisticated tools for transgenic tagging and tissue-specific functional interrogation. Many recent studies have taken advantage of these to dissect secretory traffic in an animal (Fujii et al, 2020; Glashauser et al, 2023; Johnson et al, 2020; Ma et al, 2020; Park et al, 2022; Song et al, 2022; van Leeuwen et al, 2018; Zajac and Horne-Badovinac, 2022; Zhou et al, 2023). In Drosophila , the early secretory pathway is organized into secretory units, tens to hundreds per cell, in which ERES lie in close proximity to Golgi ministacks (Kondylis and Rabouille, 2009).…”

Section: Introductionmentioning

confidence: 99%

p24-Tango1 interactions ensure ER-Golgi interface stability and efficient transport

Yang,

Feng,

Pastor-Pareja

2024

Preprint

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The eukaryotic p24 family, consisting of α-, β-, γ- and δ-p24 subfamilies, has long been known to be involved in regulating secretion. Despite increasing interest in these proteins, fundamental questions remain about their role. Here, we systematically investigatedDrosophilap24 proteins. We discovered that members of all four p24 subfamilies are required for general secretion, and that their localizations between ER exit site (ERES) and Golgi are interdependent in an α→βδ→γ sequence. We also found that localization of p24 proteins and ERES determinant Tango1 requires interaction through their respective GOLD and SH3 lumenal domains, with Tango1 loss sending p24 proteins to the plasma membrane and vice versa. Finally, we show that p24 loss expands the COPII zone at ERES and increases the number of ER-Golgi vesicles, supporting a restrictive role of p24 proteins on vesicle budding for efficient transport. Our results reveal Tango1-p24 interplay as central to the generation of a stable ER-Golgi interface.SummaryYang et al. systematically analyze inDrosophilathe function of the four p24 protein subfamilies and discover that interaction with Tango1 is essential for their concentration between ER and Golgi and for efficiency of COPII-mediated general secretory transport.

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