Acute lung inflammation induced by zinc oxide nanoparticles: Evolution and intervention via NRF2 activator

Guo, Tingyue; Fang, Xin; Liu, Yiting; Ruan, Yihui; Yu, Huimei; Wang, Xuening; Hu, Yuxin; Wang, Gang; Xu, Yuanyuan

doi:10.1016/j.fct.2022.112898

Cited by 11 publications

(6 citation statements)

References 40 publications

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“…The Nrf2-ARE is the most critical antioxidant signaling pathway in biological organisms ( Dai et al, 2022 ; Guo et al, 2022 ; Jiang et al, 2022 ; Qiu et al, 2022 ; Shen et al, 2022a ; Yan et al, 2022 ). So far, accumulating evidence has shown that a large amount of natural active substances activate Nrf2-mediated antioxidant signaling pathway and regulate the expression of cytoprotective enzymes, thus playing an important role in preventing antioxidant damage ( Eggler et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Liang

et al. 2023

Current Research in Food Science

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Section: Introductionmentioning

confidence: 99%

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Liang

et al. 2023

Current Research in Food Science

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“…Використання значно меншої дози сприяло подібним змінам -відмічали підвищення рівня МДА в легенях і зниження загальної антиоксидантної здатності (З-АОА) через 6 годин, а також підвищення активності лактатдегідрогенази в рідині бронхоальвеолярного лаважу (БАЛ) через 1 день після введення. Прояв запального процесу спостерігали через 3 і 7 днів, про що свідчило збільшення кількості лейкоцитів і концентрації загального білка в рідині БАЛ, а також гістологічні аномалії легень (Guo et al, 2022). Також встановлено збільшення рівня мРНК амілоїду A3 у сироватці крові, як маркеру гострої фази відповіді, на 1-й день дослідження та генотоксичну дію (Hadrup et al, 2019).…”

Section: вступunclassified

Acute toxicity of zinc carbonate nanocrystals on white mice model

Koshevoy,

Naumenko,

Orobchenko

et al. 2023

SMLNUVMB

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Zinc-based nanoparticles (NPs) are successfully used to meet the mineral needs of animals and poultry, capable of increasing the growth rate and the efficiency of their use of feed. Nanoparticles of zinc oxide are widely used, which are superior to conventional sources of zinc in terms of pharmacological activity and bioavailability, have a positive effect on productivity and antioxidant protection, and increase reproductive potential. However, a detailed investigation of their toxicological parameters showed the risks of their introduction into the body, as a result of which a large number of zinc oxide NPs were synthesized by the methods of “green chemistry”, the main drawback of which is a complex methodology and a high level of costs. We investigated the acute toxicity of a colloidal solution of zinc carbonate nanocrystals in a model of white mice. The experiment was conducted on 42 female non-linear white mice, 3–4 months old, with a live weight of 21–23 g, kept under optimal vivarium conditions. According to the principle of analogues, 6 experimental groups were formed: mice were injected with a colloidal solution of zinc carbonate nanoparticles in doses (by absolute weight of the drug) of 15,000.0; 20000.0; 25000.0; 30000; 35000.0 and 40000.0 mg/kg of body weight based on the absolute weight of the drug, which according to the active substance (zinc carbonate nanoparticles) was (37.5; 50.0; 62.5; 75.0; 87.5 and 100, 0 mg/kg of body weight), orally using an esophageal-gastric tube. Mice of the control group were injected with distilled water in a volume of 0.2 cm3 according to the same protocol. The clinical condition of the experimental animals was observed for 14 days, noting the appearance and development of clinical signs of poisoning, the time of death or recovery to the physiological norm. After the death (diagnostic slaughter) of the animals, a pathological autopsy was performed. Clinical observations showed that a single intragastric administration of the drug to mice of experimental groups 1-6 did not cause acute poisoning. The deaths of mice in all experimental groups were not observed during the 14-day observation period, as a result of which the LD50 indicator could not be calculated. At the same time, the maximum possible injected dose of the colloidal solution of zinc carbonate nanoparticles (by absolute weight) was 40,000.0 mg/kg of body weight (100.0 mg/kg by active substance), which allows the drug to be assigned to the VI class of toxicity – substances are relatively harmless (LD50 > 15000.0 mg/kg of body weight), and according to the degree of danger up to IV class – low-hazardous substances (LD50 > 5000.0 mg/kg of body weight).

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“…It has recently been reported that the intratracheal instillation test can be useful for acidic compounds that are difficult to test with the inhalation test (30). In addition, many studies on the toxic effects of nanomaterials using intratracheal instillation have been published (31)(32)(33). One study reported that the intratracheal instillation experiment can be used to rank the harmfulness of nanomaterials (34).…”

Section: Organ/histopathologymentioning

confidence: 99%

Acute Toxicity in the Rat Lung Induced by Intratracheal Instillation of Glycolic Acid

Lim

Kim

Yoo

et al. 2022

In Vivo

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Background/Aim: Inhalation toxicity tests of glycolic acid, which is used in many household products, have been reported, but the pulmonary toxicity of glycolic acid has not been confirmed. Here, the lung damage caused by glycolic acid was investigated in rats. Materials and Methods: An intratracheal instillation test was performed with glycolic acid in male rats. Bronchoalveolar lavage fluid (BALF) and histopathological analysis were conducted to identify the pulmonary toxicities. Results: Intratracheal instillation of glycolic acid caused weight loss in animals and increased the content of lactate dehydrogenase, total protein, polymorphonuclear neutrophils, and inflammatory cytokines in BALF. In addition, pulmonary edema, alveolar/interstitial inflammation, and necrosis and desquamation of bronchial/bronchiolar epithelia were confirmed via histopathological examination. Conclusion: Exposure to glycolic acid can be harmful and toxic to the lungs.Glycolic acid, also known as hydroxyacetic acid, is widely used in the cosmetic, textile, food, and pharmaceutical industries (1-3). The global glycolic acid market is expected to grow gradually every year (4). This means people will be more exposed to glycolic acid, via skin contact, oral intake, or inhalation. Workers dealing with glycolic acid, in particular, are more likely to be exposed to it (5).Glycolic acid was assigned to the hazard statement H332 for acute inhalation toxicity (6). The inhalation LC 50 (4-h exposure) of glycolic acid for rats has been reported to be 7.1 mg/l, and the no observed adverse effect concentration values were 0.16 mg/l and 0.05 mg/l or higher for 14-( 7) and 28-day inhalation exposure (8), respectively.There are many differences between humans and rodents. One of them is that rodents are obligatory nasal breathers, whereas humans are oronasal breathers. This means that the lower respiratory tract of humans may be more exposed to substances than that of rodents (9, 10). Animal inhalation experiments with glycolic acid have been performed, but the damage to the lower respiratory tract or the toxic effects derived from acute inhalation have not been clearly identified. Therefore, we conducted intratracheal instillation experiments to confirm the pulmonary toxicities of glycolic acid in Sprague-Dawley rats. Materials and MethodsReagents. Glycolic acid (Cas no. 79-14-1) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's Phosphate Buffered Saline (DPBS) was purchased from Lonza (Basel, Switzerland). 10% neutral buffered formalin was purchased from BBC Biochemical (McKinney, TX, USA). Isoflurane was purchased from Piramal Critical Care (Bethlehem, PA, USA).Animals and treatments. The animal study was approved by the Institutional Animal Care and Use Committee (IACUC) of the National Institute of Environmental Research (NIER) (Republic of Korea) and an IACUC-approved test protocol was followed. Male Sprague-Dawley (6-week-old) rats were purchased from Orient Bio Inc., (Seongnam, Republic of Korea). Animals were housed at room tem...

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Acute lung inflammation induced by zinc oxide nanoparticles: Evolution and intervention via NRF2 activator

Cited by 11 publications

References 40 publications

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Acute toxicity of zinc carbonate nanocrystals on white mice model

Acute Toxicity in the Rat Lung Induced by Intratracheal Instillation of Glycolic Acid

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