Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice

Abstract

The effects of exercise on nutrient digestion and absorption in the intestinal tract are not well understood. A few studies have reported that exercise training increases the expression of molecules involved in carbohydrate digestion and absorption. Exercise was also shown to increase the blood concentration of glucagon-like peptide-2 (GLP-2), which regulates carbohydrate digestion and absorption in the small intestine. Therefore, we investigated the effects of exercise on the expression of molecules involved in intestinal digestion and absorption, including GLP-2. Six-week-old male mice were divided into a sedentary (SED) and low-intensity exercise (LEx) group. LEx mice were required to run on a treadmill (12.5 m/min, 1 h), whereas SED mice rested. All mice were euthanized 1 h after exercise or rest, and plasma, jejunum, ileum, and colon samples were collected, followed by analysis via IHC, EIA, and immunoblotting. The levels of plasma GLP-2 and the jejunum expression of the GLP-2 receptor, sucrase-isomaltase (SI), and glucose transporter 2 (GLUT2) were higher in LEx mice. Thus, we showed that acute low-intensity exercise affects the expression of molecules involved in intestinal carbohydrate digestion and absorption via GLP-2. Our results suggest that exercise might be beneficial for small intestine function in individuals with intestinal frailty.

Female athlete triad affects rat intestinal morphology and sucrase-isomaltase expression

“…Jejunum samples were stained with hematoxylin and eosin (HE) according to protocols from previous studies (12,16) . Mucosal thickness, villus height, crypt depth, and villus width were measured in at least five villi/rat as previously described (17) .…”

“…qPCR was performed with the following conditions: Hold stage; 95 °C for 20 s, PCR stage; 95 °C for 1 s, 60 °C for 20 s, 40 cycles, Melt curve stage; 95 °C for 1 s, 60 °C for 20 s, 95 °C for 1 s. The 28S ribosomal RNA was used as the housekeeping gene. The primer sequences were adopted from a previous study (16) . The primer sequences for the 28S ribosomal RNA were as follows: Accepted manuscript method adapted from Livak and Schmittgen (18) and expressed relative to the levels in the Con group.…”

Glucose and Several Mitogenic Agents Modulate the Glucagon-Like Peptide-2 Receptor Expression in Cultured Rat Astrocytes

“…In addition, GLP-2R expression was also upregulated in hepatic stellate cells of mice with hepatosteatosis [ 9 ]. Likewise, GLP-2R expression was upregulated in the jejunum of mice subjected to acute exercise [ 10 ]. In turn, regulation of the GLP-2R expression has also been studied in several pathologies [ 11–14 ] and under diabetogenic conditions [ 15 ].…”