Acute liver injury upregulates microRNA-491-5p in mice, and its overexpression sensitizes Hep G2 cells for tumour necrosis factor-α-induced apoptosis

Yoon, Sangjeong; Kim, Tae Hun; Natarajan, Arutselvan; Wang, Si Si; Choi, Jeong‐Woo; Wu, Jian; Zern, Mark Α.; Venugopal, Senthil K.

doi:10.1111/j.1478-3231.2009.02181.x

Cited by 28 publications

(30 citation statements)

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“…miRNA Isolation, Labeling, Purification, and Microarray miRNA isolation, labeling, and purification were performed as we reported previously (12). Hybridized miRNA slides were scanned with a Genepix 4000B scanner (Agilent Technologies Inc., Santa Clara, CA) (12).…”

Section: Ssglt and 50% Ph In Ratsmentioning

confidence: 99%

“…The administration of nucleic acid-modified oligonucleotide complementary to miR_122 led to long-lasting hepatitis C virus suppression in infected chimpanzees without resistance (10). In previous studies, we have characterized the signatures of miRNAs in acute liver injury and hepatic fibrosis and defined the molecular targets of significantly increased or suppressed miRNAs in these processes (11,12). To our knowledge, no information regarding whether miRNAs participate in modulation of liver growth in LDLT is available in the literature.…”

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confidence: 99%

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miRNA Regulation of Liver Growth After 50% Partial Hepatectomy and Small Size Grafts in Rats

et al. 2011

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Background. The molecular mechanisms underlying the growth of small size grafts and the remaining livers are poorly understood. MicroRNAs (miRNAs) negatively modulate expression of genes that are involved in cellular function and metabolism. The aim of this study is to identify critical miRNA species that modulate the growth of small grafts and the remaining livers after partial hepatectomy (PH). Methods. Small size graft liver transplantation was performed in rats. Liver tissue was harvested after transplant or PH for the determination of miRNA expression profile, and the data were confirmed by quantitative reverse-transcriptase polymerase chain reaction. The genes involved in cell cycle and proliferation were analyzed by quantitative reversetranscriptase polymerase chain reaction and immunohistochemical staining. Results. Compared with control liver, miR_122a, Let_7b, and miR_26a were reduced by more than 90% in 45% volume grafts. In the remaining livers after 50% PH, 30 miRNAs were down-regulated by more than 50%, and among them, miR_22a, miR_26a, miR_30b, Let_7f, and Let_7g were markedly decreased. A negative correlation existed between down-regulated miRNAs and highly up-regulated genes involved in cell cycle and proliferation in the remaining livers. Moreover, overexpression of miR_26a markedly down-regulated cyclin E2 protein levels and significantly decreased proliferation of HepG2 cells. Conclusion. Down-regulated miRNAs play a pivotal role in promoting the growth of small size grafts and the remaining livers. The negative correlation between down-regulated miRNAs and up-regulated genes suggests that these specific miRNAs participate in the modulation of a growth response in both living donors and small size graft recipients.

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Section: Ssglt and 50% Ph In Ratsmentioning

confidence: 99%

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confidence: 99%

miRNA Regulation of Liver Growth After 50% Partial Hepatectomy and Small Size Grafts in Rats

et al. 2011

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“…97 These and other studies demonstrate that miRs regulate death receptor-mediated hepatocytes apoptosis in ALF. 98,99 Serum miRs that regulate hepatic apoptotic pathways may be shed into the circulation and reflect the degree of hepatic cell death could serve as reporters for progression of ALF.…”

Section: Acute Liver Failure (Alf)mentioning

confidence: 99%

MicroRNAs in Liver Disease: Bench to Bedside

Shah

Nelson

Kowdley

2013

Journal of Clinical and Experimental Hepatology

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MicroRNAs (miRs) are small non-coding RNAs that negatively regulate gene expression by pairing with partially complementary target sequences in the 3 0 UTRs of mRNAs to promote degradation and/or block translation. Aberrant miR expression is associated with development of multiple diseases including hepatic diseases. The role of miRs in the regulation of gene expression and rapid progress in the field of microRNA research are resulting in momentum toward development of diagnostic markers and novel therapeutic strategies for human liver diseases. Recent studies provide clear evidence that miRs are abundant in the liver and modulate a diverse spectrum of biological functions, thereby supporting an association between alterations of miR homeostasis and pathological liver diseases. Here we review the role of miRs in liver as their physiological and pathological importance has been demonstrated in metabolism, immunity, viral hepatitis, oncogenesis, fatty liver diseases (alcoholic and non-alcoholic), drug-induced liver injury, fibrosis as well as acute liver failure. ( J CLIN EXP HEPATOL 2013;3:231-242)

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“…MicroRNAs (miRNAs) are a family of endogenous, noncoding, small single stranded RNAs (21-25 nucleotides) that regulate the gene expression at post-transcriptional levels (Venugopal et al, 2009;Yoon et al, 2010). miRNAs bind to the target sequences by partial or complete binding to the 3' untranslated region (UTR) of their target mRNAs, and thereby mediate mRNA degradation or translational inhibition, which depends on the degree of complementarity between miRNAs and their targets (Carthew, 2006).…”

Section: Micrornasmentioning

confidence: 99%

“…Several study reports have been showed that antibodies linked to NP can detect or target cells with high specificity (Yang et al, 2007). In our recent study, we have used gold nanoparticles (AuNP) of a small size (10 nm) coated with streptavidin (SA) for conjugation of molecules to create a nanoimmunogene conjugate (Yoon et al, 2010). This conjugates includes an apoptosis-inducing miRNA (miRNA-491), breast cancer targeting MAb, and quantum dots to target breast cancer selectively and to induce apoptosis (Natarajan et al, 2009).…”

Section: Nanoparticles In Breast Cancer Translational Researchmentioning

confidence: 99%

Translational Research on Breast Cancer: miRNA, siRNA and Immunoconjugates in Conjugation with Nanotechnology for Clinical Studies

Natarajan¹,

Venugopal²

2011

Breast Cancer - Recent Advances in Biology, Imaging and Therapeutics

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Acute liver injury upregulates microRNA-491-5p in mice, and its overexpression sensitizes Hep G2 cells for tumour necrosis factor-α-induced apoptosis

Abstract: One of the miRNAs that is associated with the acute liver injury mouse model, miRNA-491_5p, sensitizes Hep G2 cells for TNF-alpha-induced apoptosis, at least in part, by inhibiting AFP, hsp-90 and NF-kappaB.

Cited by 28 publications

References 46 publications

miRNA Regulation of Liver Growth After 50% Partial Hepatectomy and Small Size Grafts in Rats

miRNA Regulation of Liver Growth After 50% Partial Hepatectomy and Small Size Grafts in Rats

MicroRNAs in Liver Disease: Bench to Bedside

Translational Research on Breast Cancer: miRNA, siRNA and Immunoconjugates in Conjugation with Nanotechnology for Clinical Studies

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