Acute liver failure in an immunocompetent patient

Paula, Vanessa Salete de; Silva, Amanda Perse da; Michel, Detlef; Melgaço, Juliana Gil; Vieira, Yasmine Rangel; Santos, Damião Carlos Dos; Araújo, Cristina Carvalho Viana de; Pacheco-Moreira, L.F.; Pelajo-Machado, Marcelo; Nascimento, Cristina Moreira do; Schmidt‐Chanasit, Jonas; Pinto, Marcelo Alves

doi:10.1016/j.jcv.2014.03.008

Cited by 6 publications

(14 citation statements)

References 13 publications

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“…Among the samples positive for betaherpesviruses, 66% were HHV-6-positive. The greater positivity for HHV-6 corroborates with previous results showing that HHV-6 is more prevalent and pathogenic in ALF cases (9,13). In another study, ALF was associated with primary HHV-6 infection in one child (27).…”

Section: Detection Via Pan Herpesvirus Pcr and Sequencingsupporting

confidence: 91%

“…However, in immunocompromised cases, in particular, organ transplant recipients, HCMV infection is associated with increased morbidity (7) Primary infection with HHV-6 is a frequent cause of ALF, necessitating antiviral therapy during early post-transplantation procedures. A number of studies have disclosed the presence of HHV-6 in liver samples from pretransplant cases (8,9). Complications resulting from HHV-7 infection have also been identified as a risk in organ transplantation (10).…”

Section: Introductionmentioning

confidence: 99%

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MULTIPLEX qPCR FACILITATES IDENTIFICATION OF BETAHERPESVIRUSES IN PATIENTS WITH ACUTE LIVER FAILURE OF UNKNOWN ETIOLOGY

Raposo

Alves

Silva

et al. 2019

Preprint

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Background: The etiology of acute liver failure (ALF) is often unknown and reported to be associated with herpesviruses in a number of cases. In this study, we examined for betaherpesviruses infections in patients with ALF of unknown etiology using a multiplex qPCR to Betaherpesviruses subfamily. Methods: Liver explant and serum samples from 27 patients with ALF of unknown etiology were analyzed with the aid of multiplex qPCR to identify betaherpesviruses. All positive samples were sequenced to confirm herpes infection and liver enzyme levels evaluated. Results: Betaherpesviruses infection was effectively detected using multiplex qPCR. Six (22%) HHV-6, one (3%) HCMV and two (7%) dual infections (one with HHV-7/HHV-6, and the other with HHV-7/ HCMV). Interestingly, HHV-7 was only detected in the presence of other betaherpesviruses. Sequencing information confirmed betaherpesviruses infection. High hepatic enzyme levels and INR values>1.5 were determined in all betaherpesvirus-positive patients. Conclusions: Multiplex qPCR facilitated efficient quantification, indicating that differentiation between betaherpesviruses is possible with the sole use of real-time PCR. Liver explant and serum samples were positive for some betaherpesviruses, and coinfection of HHV-7 with HHV-6 and HCMV was additionally detected. Based on these results, we propose that ALF patients should be screened for the presence of betaherpesviruses.

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Section: Detection Via Pan Herpesvirus Pcr and Sequencingsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

MULTIPLEX qPCR FACILITATES IDENTIFICATION OF BETAHERPESVIRUSES IN PATIENTS WITH ACUTE LIVER FAILURE OF UNKNOWN ETIOLOGY

Raposo

Alves

Silva

et al. 2019

Preprint

Self Cite

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show abstract

“…Among the samples positive for betaherpesviruses, 66% were HHV-6-positive. The greater positivity for HHV-6 corroborates with previous results showing that HHV-6 is more prevalent and pathogenic in ALF cases (9,26). In another study, ALF was associated with primary HHV-6 infection in one child (27).…”

Section: Reference Controlsupporting

confidence: 91%

“…A number of studies have disclosed the presence of HHV-6 in liver samples from pretransplant cases (8,9). Complications resulting from HHV-7 infection have also been identified as a risk in organ transplantation (10).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, real-time PCR has been increasingly applied to simplify the overall procedure and reduce performance time in detecting and quantifying virus in clinical samples (12,13). Multiplex qPCR has been suggested as an efficient alternative for simultaneous detection of HCMV, HHV-6 and HHV-7 within the same reaction (9,14). In the current study, the incidence of betaherpesvirus infection in patients with ALF of unknown etiology was investigated using the multiplex qPCR approach.…”

Section: Introductionmentioning

confidence: 99%

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Multiplex Qpcr Facilitates Identification Of Betaherpesviruses In Patients With Acute Liver Failure Of Unknown Etiology

Raposo

Alves

Silva

et al. 2019

Preprint

Self Cite

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Background: The etiology of acute liver failure (ALF) is often unknown and reported to be associated with herpesviruses in a number of cases. In this study, we examined for betaherpesviruses infections in patients with ALF of unknown etiology using a multiplex qPCR to Betaherpesviruses subfamily. Methods: Liver and serum samples from 27 patients with ALF of unknown etiology were analyzed with the aid of multiplex qPCR to identify betaherpesviruses. All positive samples were sequenced to confirm herpes infection and liver enzyme levels evaluated. Results: Betaherpesviruses infection was effectively detected using multiplex qPCR. Seven (26%), two (7%) and three (11%) cases were positive for HHV-6, HHV-7 and HCMV, respectively. Two cases of dual infection (HHV-7/HHV-6 and HHV-7/HCMV) were additionally identified. Interestingly, HHV-7 was only detected in the presence of other betaherpesviruses. Sequencing information confirmed betaherpesviruses infection. High hepatic enzyme levels and INR values>1.4 were determined in all betaherpesvirus-positive patients. Conclusions: Multiplex qPCR facilitated efficient quantification, indicating that differentiation between betaherpesviruses is possible with the sole use of real-time PCR. Liver and serum samples were positive for some betaherpesviruses, suggesting an association with ALF. Coinfection of HHV-7 with HHV-6 or HCMV was additionally detected, suggesting that the precursor betaherpesviruses infection can trigger HHV-7 infection. Based on these results, we propose that ALF patients should be screened for the presence of betaherpesviruses.

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Triple Infection by Betaherpesviruses (HCMV, HHV-6 and HHV-7) in a Child with Acute Liver Failure

et al. 2021

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Acute liver failure in an immunocompetent patient

Cited by 6 publications

References 13 publications

MULTIPLEX qPCR FACILITATES IDENTIFICATION OF BETAHERPESVIRUSES IN PATIENTS WITH ACUTE LIVER FAILURE OF UNKNOWN ETIOLOGY

MULTIPLEX qPCR FACILITATES IDENTIFICATION OF BETAHERPESVIRUSES IN PATIENTS WITH ACUTE LIVER FAILURE OF UNKNOWN ETIOLOGY

Multiplex Qpcr Facilitates Identification Of Betaherpesviruses In Patients With Acute Liver Failure Of Unknown Etiology

Triple Infection by Betaherpesviruses (HCMV, HHV-6 and HHV-7) in a Child with Acute Liver Failure

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