Acute Kidney Injury Associated with Hepato-protective Chinese Herb – Pien Tze Huang

Lin, Yen‐Chung; Chen, Yen Cheng; Chen, Tso Hsiao; Chen, Hsi Hsien; Tsai, Wei‐Jern

doi:10.1016/j.jecm.2011.07.005

Cited by 5 publications

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References 9 publications

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“…additional studies have also suggested that PZH is effective in inhibiting liver damage caused by excessive drinking (16), improving histopathological damage in the liver and positively affecting physiological and biochemical indexes, such as serum alanine aminotransferase and aspartate aminotransferase, to a certain extent (15,17). Cell necrosis and swelling, microvesicular steatosis and lymphocyte infiltration in the injured liver have been reported to be significantly reduced following PZH treatment for liver disease (18). circular rnas (circrnas) are a recently discovered type of non-coding RNAs (ncRNAs).…”

Section: Introductionmentioning

confidence: 99%

Identification of circular RNA biomarkers for Pien Tze Huang treatment of CCl4‑induced liver fibrosis using RNA‑sequencing

et al. 2022

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Pien Tze Huang (PZH), a common hepatoprotective Traditional chinese Medicine that has been found to be an effective treatment for carbon tetrachloride-induced hepatic damage, including liver fibrosis. Circular RNAs (circRNAs) serve a crucial role in regulating gene expression levels via circrna/micro (mi)rna/mrna networks in several human diseases and biological processes. However, whether circrnas are involved in the underlying mechanism of the therapeutic effects of PZH on liver fibrosis remains unclear. Therefore, the aim of the present study was to investigate these effects using circRNA expression profiles from PZH-treated fibrotic livers in model mice. A case-control study on >59,476 circrnas from ccl 4 -induced (control group, n=6) and PZH-treated (case group, n=6) mice was performed using circRNA sequencing in liver tissues. PZH treatment resulted in the differential expression of 91 circrnas, including 58 upregulated and 33 downregulated circRNAs. Furthermore, the construction of competing endogenous networks also indicated that differentially expressed circrnas acted as mirna sponges. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of miRNA targets demonstrated that PZH-affected circrnas were mainly involved in biological processes such as 'positive regulation of fibroblast proliferation', 'cellular response to interleukin-1' and 'regulation of dna-templated transcription in response to stress' and in a number of important pathways, such as 'TNF signaling pathway', 'PI3K-Akt signaling pathway', 'IL-17 signaling pathway' and 'MAPK signaling pathway'. To further validate the bioinformatics data, reverse transcriptionquantitative Pcr was performed on seven mirna targets in a human hepatic stellate LX-2 cell model. The results suggested that seven of the mirnas exhibited regulatory patterns that were consistent with those of the transcriptome sequencing results. Kaplan-Meier survival analysis demonstrated that the expression levels of dihydrodiol dehydrogenase and solute carrier family 7, member 11 gene were significantly associated with patient survival, 269 patients with liver hepatocellular carcinoma from The Cancer Genome Atlas database. To the best of our knowledge, this was the first study to provide evidence that PZH affects circrna expression levels, which may serve important roles in PZH-treated fibrotic liver through the regulation of functional gene expression. In conclusion, the

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