Acute Kidney Injuries Induced by Various Irrigation Pressures in Rat Models of Mild and Severe Hydronephrosis

Cao, Zhixiu; Ye, Weimin; Li, Wei; Xia, Yunlong; Rao, Ting; Yao, Xiaolan; Zhang, Xiaobin; Larré, S.

doi:10.1016/j.urology.2013.08.024

Cited by 9 publications

(7 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that kidneys with hydronephrosis are more likely to suffer hypoxia problems (26,27). According to our previous study, rabbit kidneys with severe hydronephrosis suffered acute kidney injury more readily compared with those with mildly nephrotic kidneys when exposed to pneumoperitoneal pressure (28). Another study demonstrated that severe hydronephrosis (≥grade 3) led to prolonged pneumoperitoneum time and total operation time in laparoscopic radical nephroureterectomy (29).…”

Section: Discussionmentioning

confidence: 85%

Oxidative damage and mitochondrial injuries differ following pneumoperitoneum pressure in rabbit models of varying degrees of hydronephrosis

Zhao

Rao

et al. 2018

Mol Med Report

Self Cite

View full text Add to dashboard Cite

The influence of intraabdominal pressure which is necessary to maintain the operating area during the surgery cannot be ignored especially on the kidneys. Many articles have reported the effect of intraabdominal pressure on normal kidneys. However, the influence of intraabdominal pressure on hydronephrosis kidneys is rarely studied. The aim of the present study was to clarify whether intraabdominal pressure tolerance is modified in various degrees of kidney hydronephrosis by evaluating oxidative damage and mitochondrial injuries. A total of 72 rabbits were randomly divided into three groups (groups N, M and S, which represented rabbits with no, mild and severe hydronephrosis, respectively). Rabbits in groups M (n=24) and S (n=24) underwent a surgical procedure inducing mild or severe hydronephrosis, respectively. Subsequently, rabbits in all groups were allocated to 4 subgroups (N0‑N3, M0‑M3 and S0‑S3) consisting of 6 rabbits each. Groups 0 to 3 were, respectively, subjected to intraabdominal pressures of 0, 5, 10 and 15 mmHg. Oxidative damage was assessed by analyzing levels of reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH‑Px), catalase (CAT) and lactate (LD). Mitochondrial injuries were assessed based on mitochondrial membrane potential (MMP) alterations, mitochondrial structure and cytochrome c (cytc) protein expression, as measured by JC‑1 staining, electron microscopy and western blotting, respectively. Oxidative damage and mitochondrial injuries were noticeably exacerbated in group N and M with increased levels of ROS, MDA and LD, decreased levels of SOD, GSH‑Px, CAT and MMP, mitochondrial vacuolization and higher expression of cytc when the intraabdominal pressure reached 15 mmHg. In group S, these alterations occurred at pressures of 10 and 15 mmHg. Therefore, it was concluded that in rabbits exposed to pneumoperitoneal pressure, kidneys with severe hydronephrosis were more likely to suffer from oxidative damage and mitochondrial injuries compared with kidneys with mild hydronephrosis and normal kidneys.

show abstract

Section: Discussionmentioning

confidence: 85%

Oxidative damage and mitochondrial injuries differ following pneumoperitoneum pressure in rabbit models of varying degrees of hydronephrosis

Zhao

Rao

et al. 2018

Mol Med Report

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a previous experiment, it was observed that kidney damage became serious when the degree of perfusion pressure of severe hydronephrosis reached 60 mmHg [6]. Kidney damage differed depending on whether the renal pelvis was perfused with pressures of 20 or 60 mmHg.…”

Section: Discussionmentioning

confidence: 93%

“…Venous outflow obstruction can result in the formation of microthrombosis, which then affects the blood supply of the renal parenchyma. Essentially, kidney damage becomes an ischemic injury when severe hydronephrosis is subjected to a certain degree of perfusion [4,6].…”

Section: Discussionmentioning

confidence: 99%

Protective effect of cyclosporine A in the treatment of severe hydronephrosis in a rabbit renal pelvic perfusion model

ZHOU

Zhang

et al. 2019

Turk J Med Sci

View full text Add to dashboard Cite

Introduction With the development of minimally invasive technology, many types of ureteroscopy and percutaneous nephroscope lithotripsy have become routine procedures in surgical intervention for kidney stones, as they have several advantages, including reduced postsurgical pain, efficient stone clearance, shorter hospitalization time, and reduced scar formation than open surgery [1,2]. However, minimally invasive surgery in the kidney may not be minimally invasive within the kidney itself. Endourological operations need sufficient fluid perfusion to clearly flush out kidney stone fragments during these procedures, due to the limitations of the orifices [3]. These procedures can cause high intrapelvic pressure and pyelovenous backflow when the pressure increases to a certain extent, which could reduce renal arterial perfusion and lead to renal ischemic injury [4,5]. In addition, there is a certain degree of hy-dronephrosis in patients with upper urinary tract stones. A previous study demonstrated that a 60 mmHg renal pelvic perfusion substantially aggravated kidney damage in a rabbit model of hydronephrosis via mitochondrial injury [6]. Nevertheless, effective protection for hydronephrotic kidneys after renal pelvic perfusion has not yet been studied. Cyclosporine A (CsA), known as an immunosuppressive compound, has been traditionally used to prevent and treat transplant rejection [7]. Recently, CsA has been thought to specifically prevent mitochondrial permeability transition pore (mPTP) opening and attenuate cell apoptosis by exerting cardioprotective effects in a reperfusion injury model [8]. Moreover, previous studies have shown that CsA protects against tissue ischemia-reperfusion injury in the brain [9], lung [10], and kidney [11] in vivo. However, whether CsA affects renal pelvic perfusion-in-Background/aim: Cyclosporine A (CsA), a traditional immunosuppressive compound, has been reported to specifically prevent ischemia reperfusion tissue injury via apoptosis pathway. This study aimed to explore the renoprotective effects of CsA on the kidneys of rabbits undergoing renal pelvic perfusion. Materials and methods: A total of 30 rabbits were randomly assigned into a control group (n = 6) and an experimental group (n = 24). The experimental group underwent a surgical procedure that induced severe hydronephrosis and was then stochastically divided into 4 groups (S1, S1' , S2, and S2'), consisting of 6 rabbits each. Groups S1 and S1' were perfused with 20 mmHg of fluid, while groups S2 and S2' were perfused with 60 mmHg of fluid. Administration to groups S1' and S2' was done intravenously, with CsA once a day for 1 week before perfusion. In the control group, after severe hydronephrosis was induced, a sham operation was performed in a second laparotomy. Acute kidney damage was evaluated using hematoxylin and eosin staining, in addition to analyzing the mitochondrial ultrastructure and mitochondrial membrane potential (MMP). The cytochrome C (CytC) and neutrophil gelatinase-associated lipocalin (NGAL) expression ...

show abstract

“…The resulting increase in local cytokine and growth factor release may lead to tubular atrophy, nephron loss and accumulation of fibrotic interstitial tissue [ 63 , 64 ]. Thus it could be hypothesized that increased IRPs frequently observed during RIRS could induce renal injury similar to the way in which elevated IRPs provoke renal injury in animal models of obstructive uropathy [ 65 ]; however, no study thus far has examined this specific hypothesis of possible RIRS-induced damage. The use of ureteral access sheath seems to be the most efficient measure to avoid IRPs that may be harmful during RIRS [ 6 ]; again, whether this can be protective against renal injury and a decrease in renal function is not known.…”

Section: Effects Of Endourological Procedures On Renal Function Measumentioning

confidence: 99%

“…Further research on the effect of endourological procedures on renal injury and renal function is clearly needed. As discussed, different pathophysiological mechanisms leading to renal injury for each endourological procedure have been proposed [ 4 , 6 , 34 , 65 ]; however, as of this writing, details on the nature and extent of renal injury during each of these procedures is still lacking, possibly because of the inappropriate structure of the studies (e.g. lack of a modern definition of AKI, use of only conventional indexes of renal function instead of markers of renal injury, incorrect timing of measurements, small sample size, short follow-up, incorrect or absent reporting of surgical parameters, etc.).…”

Section: Further Research Agendamentioning

confidence: 99%

Are endourological procedures for nephrolithiasis treatment associated with renal injury? A review of potential mechanisms and novel diagnostic indexes

Mykoniatis

Sarafidis

Memmos

et al. 2020

Clinical Kidney Journal

View full text Add to dashboard Cite

Nephrolithiasis is one of the most common urological conditions with a huge socio-economic impact. About 50% of recurrent stone-formers have just one lifetime recurrence and >10% of patients present with a high recurrent disease requiring subsequent and sometimes multiple surgical interventions. The advent of new technology has made endourological procedures the pinnacle of stone treatment, including procedures like percutaneous nephrolithotomy (PCNL), retrograde intrarenal surgery and miniaturized PCNL procedures. Researchers have primarily focused on comparisons with respect to stone-free rates, procedure parameters and post-operative complications. However, the effect of these three procedures on renal function or indexes of renal injury has not been sufficiently examined. This was only reported in a few studies as a secondary objective with the use of common and not the appropriate and detailed renal parameters. This review presents current literature regarding the use of novel and highly predictive biomarkers for diagnosing acute kidney injury, discusses potential mechanisms through which endourological procedures for renal stone treatment may affect renal function and proposes areas with open questions where future research efforts in the field should focus.

show abstract

Acute Kidney Injuries Induced by Various Irrigation Pressures in Rat Models of Mild and Severe Hydronephrosis

Cited by 9 publications

References 25 publications

Oxidative damage and mitochondrial injuries differ following pneumoperitoneum pressure in rabbit models of varying degrees of hydronephrosis

Oxidative damage and mitochondrial injuries differ following pneumoperitoneum pressure in rabbit models of varying degrees of hydronephrosis

Protective effect of cyclosporine A in the treatment of severe hydronephrosis in a rabbit renal pelvic perfusion model

Are endourological procedures for nephrolithiasis treatment associated with renal injury? A review of potential mechanisms and novel diagnostic indexes

Contact Info

Product

Resources

About