Acute joint pathology and synovial inflammation is associated with increased intra-articular fracture severity in the mouse knee

Lewis, John S.; Hembree, Walter C; Furman, Bridgette D.; Tippets, L.; Cattel, D.; Huebner, Janet L.; Little, Dianne; DeFrate, Louis E.; Kraus, Virginia B.; Guilak, Farshid; Olson, Steven A.

doi:10.1016/j.joca.2011.04.011

Cited by 178 publications

(172 citation statements)

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“…Then we used a summed OARSI score (0−12) from medial femoral condyle and medial tibial plateau to evaluate the degree of articular cartilage destruction. The severity of synovitis was graded using a scoring system which was previously described [26]: Enlargement of the synovial lining cell layer on a scale of 0-3 (0 = 1-2 cells, 1 = 2-4 cells, 2 = 4-9 cells and 3 = 10 or more cells) and density of cells in the synovial stroma on a scale of 0-3(0 = normal cellularity, 1 = slightly increased cellularity, 2 = moderately increased cellularity and 3 = greatly increased cellularity). We applied AxioVision software to measure the thickness of the medial subchondral bone plate according to Safranin-O-stained sections.…”

Section: Histological Analysismentioning

confidence: 99%

The Protective Effect of Ligustilide in Osteoarthritis: An in Vitro and in Vivo Study

et al. 2018

Cell Physiol Biochem

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Background/Aims: Osteoarthritis is a degenerative joint disease characterized by cartilage degeneration and a chondrocyte inflammatory response that induces an inflammatory environment closely linked to extracellular matrix (ECM) degradation. Ligustilide (LIG) is a major component of the herb Radix Angelicae Sinensis, with demonstrated anti-inflammatory effects. To confirm whether LIG has an equally inhibitory effect on inflammation in human osteoarthritis chondrocytes, we performed in vivo and in vitro experiments to validate the above conjectures and determine the relevant mechanisms. Methods: Quantitative realtime PCR and western blotting were performed to evaluate the expression of MMP-3, MMP-13, ADAMTS-5, iNOS, and COX-2 at both gene and protein levels. An enzyme-linked immunosorbent assay was used to evaluate the levels of other inflammatory factors (PGE2, TNF-α, and IL-6). The PI3K/AKT and nuclear factor kappa B (NF-κB) signaling pathways were also analyzed by western blotting, whereas immunofluorescence was used to assess the expression of collagen II and aggrecan. The in vitro effect of LIG was evaluated by intraperitoneal injection into a mouse osteoarthritis model induced by destabilization of the medial meniscus. Results: LIG lowered the phosphorylation levels of p65, IκBα, and IKKα/β and suppressed the IL-1β-induced expression of MMP-3, ADAMTS-5, iNOS, and COX-2 and the inflammatory factors PGE2, TNF-α, and IL-6. LIG markedly decreased IL-1β-induced degradation of collagen II and aggrecan. In vivo results showed that LIG-treated mouse cartilage showed less damage than the control group; the Osteoarthritis Research Society International (OARSI) score was also lower. LIG further reduced the thickness of the subchondral bone plate and alleviated the synovitis. Conclusion: LIG may act as a promising therapeutic agent for osteoarthritis by attenuating IL-1β-induced inflammation in chondrocytes and ECM degradation via suppression of NF-κB activation by the PI3K/AKT pathway.

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Section: Histological Analysismentioning

confidence: 99%

The Protective Effect of Ligustilide in Osteoarthritis: An in Vitro and in Vivo Study

et al. 2018

Cell Physiol Biochem

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“…6). 33 Lewis et al 34 used C57BL/6 mice to mold a humanoid PTOA model using the artificial compression axial overload method to cause an animal tibial plate fracture. Due to equipment limitations (such as a special tripod and compression table) and the corresponding internal fixation materials, the authors do not recommend this method of establishing the models.…”

Section: Intra-articular Fracture (Iaf) Model (3/34)mentioning

confidence: 99%

How to establish an expected animal model of post-traumatic osteoarthritis?

Han¹,

Chen²,

Tian³

et al. 2017

Chinese Nursing Research

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a b s t r a c tBackground: Animal models of osteoarthritis (OA), including post-traumatic osteoarthritis and spontaneous osteoarthritis, have been established in many ways. In recent years, there have been many reports in various foreign academic journals, but animal models of post-traumatic osteoarthritis (distinct from spontaneous osteoarthritis) have rarely been established or summarized in these reports. Animal models of post-traumatic osteoarthritis show different characteristics depending on the animal species and modeling methods used, which is why we have written this article. Objective: To summarize the research progress and research status of animal models of post-traumatic osteoarthritis. Methods: A retrospective review of the animal model of post-traumatic osteoarthritis (OA) was conducted on the basis of reports retrieved from the PubMed database with the keywords for searching "animal model, post-traumatic osteoarthritis (PTOA)" from October 2006 to October 2016 and confided English language. A total of 80 academic articles on the study of animal models of traumatic osteoarthritis were retrieved, and 34 of them were included in this literature review after reading the free fulltext of them. Results: Different PTOA models based on different modeling methods and different animal species had their own characteristics. Different modeling methods should be selected according to different modeling animals. Conclusions: Considering the project funds, experimental objectives and technical conditions, appropriate experimental animal and modeling method should be selected based on synthetic considerations to obtain an appropriate PTOA model and ideal experimental results.

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“…These include apoptosis of articular chondrocytes with subsequent loss of viable chondrocytes to support the cartilage matrix, and activation of the inflammatory cascade within the joint leading to degenerative changes in the cartilage [3,5,8]. Recent studies using a murine closed joint fracture model suggest that an intra-articular response to joint injury with increased inflammation is associated with the development of PTA and that this response increases with severity of injury [9,13]. However, very little is known concerning the acute intra-articular response following articular fracture in humans.…”

Section: Etiology Of Post-traumatic Arthritismentioning

confidence: 99%

“…In a follow-up study, we examined the influence of articular fracture severity on chondrocyte viability, morphological bone changes, circulating levels of inflammatory cytokines and cartilage and synovium derived biomarkers, and synovial changes after fracture [13]. Two groups of mice (C57BL/6) were subjected to articular fractures using the above method, and both simple and complex fractures of the tibial plateau were created.…”

Section: Experimental Models Of Post-traumatic Arthritismentioning

confidence: 99%