Acute Isolation of Neurons Suitable for Patch-Clamping Study from Frontal Cortex of Mice

Li, Yuanyuan; Cheng, Lijun; Li, Gang; Li, Dandan

doi:10.1007/978-3-642-15615-1_72

Cited by 1 publication

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then the cover glass was plated onto a dish mounted on the stage of a microscope (Olympus IX71) and the extracellular bath solution was added into the dish. Pyramidal cells in hippocampal CA1 region with uniformly bright appearance and good adhesion to cover glass were selected for patch-clamp record (Li et al, 2004).…”

Section: Neuron Acute Dissociation Proceduresmentioning

confidence: 99%

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca²⁺‐activated potassium channels

Lin

Wang

et al. 2021

Brain and Behavior

View full text Add to dashboard Cite

Acupuncture has a good effect on improving neurological function after cerebral ischemia-reperfusion, but there are few studies on the neuroprotective effect of acupuncture from the perspective of ion channel cellular electrophysiology. Studies have shown that the over activation of large-conductance Ca 2+ -activated potassium channel (BKCa) after cerebral ischemia-reperfusion can reduce the excitability of neurons and induce apoptosis. This study intends to establish middle cerebral artery occlusion/reperfusion (MCAO/R) model, with acupuncture at GV26 as the intervention measure, using patch-clamp technique to record the electrophysiological changes of BKCa channel. The results showed that the neurological function score of MCAO/R rats was significantly decreased, and the conductance, open dwell time and open probability of BKCa channel in hippocampal CA1 neurons of MCAO/R rats were significantly increased. Acupuncture at GV26 could significantly improve the neurological function scores of MCAO/R rats, and reduce the conductance, open dwell time, andopen probability of BKCa channel. The effect of acupuncture at GV26 was significantly better than acupuncture at non-acupuncture point. The neuroprotective effect of acupuncture at GV26 after cerebral ischemia-reperfusion may be related to regulating the electrophysiological characteristics of BKCa channel opening. K E Y W O R D Sacupuncture, cellular electrophysiology, cerebral ischemia-reperfusion, GV26, large-conductance Ca 2+ -activated potassium channels (BKCa), patch-clamp INTRODUCTIONStroke is one of the most common diseases in the world endangering human life and health, and 87% of stroke patients are ischemic stroke caused by vascular occlusion (Powers et al., 2018). The mortality and disability rate of ischemic stroke is high, which greatly affects the liv-This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

Section: Neuron Acute Dissociation Proceduresmentioning

confidence: 99%

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca²⁺‐activated potassium channels

Lin

Wang

et al. 2021

Brain and Behavior

View full text Add to dashboard Cite

show abstract

Acute Isolation of Neurons Suitable for Patch-Clamping Study from Frontal Cortex of Mice

Cited by 1 publication

References 3 publications

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca²⁺‐activated potassium channels

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca²⁺‐activated potassium channels

Contact Info

Product

Resources

About

Acute Isolation of Neurons Suitable for Patch-Clamping Study from Frontal Cortex of Mice

Cited by 1 publication

References 3 publications

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca2+‐activated potassium channels

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca2+‐activated potassium channels

Contact Info

Product

Resources

About

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca²⁺‐activated potassium channels

Acupuncture ameliorates neurological function in rats with cerebral ischemia‐reperfusion by regulating the opening of large‐conductance Ca²⁺‐activated potassium channels