Acute involution in the tammar wallaby: Identification of genes and putative novel milk proteins implicated in mammary gland function

Khalil, Elie; Digby, Matthew R.; Thomson, Peter; Lefèvre, Christophe; Mailer, Sonia; Pooley, Cate; Nicholas, Kevin R.

doi:10.1016/j.ygeno.2011.03.003

Cited by 13 publications

(12 citation statements)

References 58 publications

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“…It has been observed that LALBA expression is strongly induced by the onset of lactation in several species (27), decreases in late lactation in bovine (27), and further decreases at the onset of involution as observed in bovine and kangaroo (111,171). The expression of LALBA increases upon treatment with a cocktail of lactogenic hormones (i.e., insulin ϩ hydrocortisone ϩ prolactin ϩ 17␤-estradiol or growth hormone), as observed in mouse and bovine mammary cells in vitro (105,199).…”

Section: Transcriptional Regulation Of the Lactose Synthase Enzymes Lmentioning

confidence: 87%

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Osorio

Lohakare

Bionaz

2016

Physiological Genomics

182

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Section: Transcriptional Regulation Of the Lactose Synthase Enzymes Lmentioning

confidence: 87%

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Osorio

Lohakare

Bionaz

2016

Physiological Genomics

182

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“…ELP gene expression in the tammar mammary gland was investigated by analysing a microarray database [69,102-104] produced from custom-made cDNA microarray slides and total RNA collected from glands at each phase of the lactation cycle [69,102-104]. Glass microarray slides were printed by the Peter MacCallum Cancer Centre Microarray Core Facility, Melbourne, Australia and contained 10,368 tammar cDNA spots which were derived from a commercially prepared (Life Technologies, Rockville, MD, USA), normalised 15,001 tammar mammary gland EST (expressed sequence tag) library.…”

Section: Methodsmentioning

confidence: 99%

“…These included: day 26P, d55L, d87L, d130L, d180L, d220L, d260L and d5I (tissue from a d45L female 5 days after removal of the pouch young (RPY)) [19]. Gene expression changes in the tammar mammary gland during the reproductive cycle were investigated by a large-scale microarray experiment involving 36 comparisons (72 slides including dye swaps, 144 channels in total) [69,102-104]. …”

Section: Methodsmentioning

confidence: 99%

“…Microarray probes were prepared from total RNA (50 μg per sample) using a two-step procedure which involved incorporation of aminoallyl-modified dUTP and then coupling with either Cy3 or Cy5 fluorescent dye [102,104]. Slides were hybridised overnight (14–16 hr) in a humidified chamber [102,104], scanned (Agilent scanner) and the images analysed with Versarray software (Bio-Rad).…”

Section: Methodsmentioning

confidence: 99%

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The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene

et al. 2012

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BackgroundThe marsupial early lactation protein (ELP) gene is expressed in the mammary gland and the protein is secreted into milk during early lactation (Phase 2A). Mature ELP shares approximately 55.4% similarity with the colostrum-specific bovine colostrum trypsin inhibitor (CTI) protein. Although ELP and CTI both have a single bovine pancreatic trypsin inhibitor (BPTI)-Kunitz domain and are secreted only during the early lactation phases, their evolutionary history is yet to be investigated.ResultsTammar ELP was isolated from a genomic library and the fat-tailed dunnart and Southern koala ELP genes cloned from genomic DNA. The tammar ELP gene was expressed only in the mammary gland during late pregnancy (Phase 1) and early lactation (Phase 2A). The opossum and fat-tailed dunnart ELP and cow CTI transcripts were cloned from RNA isolated from the mammary gland and dog CTI from cells in colostrum. The putative mature ELP and CTI peptides shared 44.6%-62.2% similarity. In silico analyses identified the ELP and CTI genes in the other species examined and provided compelling evidence that they evolved from a common ancestral gene. In addition, whilst the eutherian CTI gene was conserved in the Laurasiatherian orders Carnivora and Cetartiodactyla, it had become a pseudogene in others. These data suggest that bovine CTI may be the ancestral gene of the Artiodactyla-specific, rapidly evolving chromosome 13 pancreatic trypsin inhibitor (PTI), spleen trypsin inhibitor (STI) and the five placenta-specific trophoblast Kunitz domain protein (TKDP1-5) genes.ConclusionsMarsupial ELP and eutherian CTI evolved from an ancestral therian mammal gene before the divergence of marsupials and eutherians between 130 and 160 million years ago. The retention of the ELP gene in marsupials suggests that this early lactation-specific milk protein may have an important role in the immunologically naïve young of these species.

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“…Similarly, the use of a marsupial-specific microarray to investigate differential gene expression has identified 47 genes upregulated during mammary gland involution. Among these are genes that could potentially play a protective role against mammary gland infection, such as ABP1, C1QB, C4A, and CSF2R β [97]. …”

Section: Lactation Genesmentioning

confidence: 99%

Marsupial Genome Sequences: Providing Insight into Evolution and Disease

Deakin

2012

Scientifica

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Marsupials (metatherians), with their position in vertebrate phylogeny and their unique biological features, have been studied for many years by a dedicated group of researchers, but it has only been since the sequencing of the first marsupial genome that their value has been more widely recognised. We now have genome sequences for three distantly related marsupial species (the grey short-tailed opossum, the tammar wallaby, and Tasmanian devil), with the promise of many more genomes to be sequenced in the near future, making this a particularly exciting time in marsupial genomics. The emergence of a transmissible cancer, which is obliterating the Tasmanian devil population, has increased the importance of obtaining and analysing marsupial genome sequence for understanding such diseases as well as for conservation efforts. In addition, these genome sequences have facilitated studies aimed at answering questions regarding gene and genome evolution and provided insight into the evolution of epigenetic mechanisms. Here I highlight the major advances in our understanding of evolution and disease, facilitated by marsupial genome projects, and speculate on the future contributions to be made by such sequences.

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Acute involution in the tammar wallaby: Identification of genes and putative novel milk proteins implicated in mammary gland function

Cited by 13 publications

References 58 publications

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene

Marsupial Genome Sequences: Providing Insight into Evolution and Disease

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