“…Several authors have tested different mercury concentrations administered through diet, ambient water or intraperitoneal injection, using both acute and chronic tests; different parameters have been used to assess the effect of this pollutant on fish liver ( i.e. , gene expression, proteomic, transcriptome analysis, DNA microarray, biochemical) [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ], but very few studies have focused on histological and ultrastructural alterations [ 9 , 22 , 30 , 31 ]. Studies on mercury have mainly focused on methyl mercury (MeHg) [ 9 , 13 , 14 , 15 , 23 , 30 ], and the iHg effects have been substantially underestimated despite the evidence that this form mainly accumulates in the kidney and in the liver of fishes [ 32 , 33 , 34 ].…”