Acute Inflammation Following Intraperitoneal Injection of Antigen into Actively Sensitised Rats

Sharpe, T. J.; Harling, Elizabeth J.; Smith, Harry A.

doi:10.1159/000232292

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…The early phase of the reaction, perhaps mediat ed by IgE or IgG»., [2,10], involves the re lease of histamine and SRS-A with an asso ciated extravasation of plasma proteins. The later phase of the reaction, perhaps me diated by humoral antibody and comple ment, consists of an infiltration of inflam matory cells, especially of neutrophils [12].…”

Section: Discussionmentioning

confidence: 99%

“…The methods were essentially as described pre viously [12]. Briefly, rats were sensitised by inject ing 50 /ig BSA in Freund's complete adjuvant (FCA) intradermally, followed 28 days later by 100 ug BSA in saline subcutaneously.…”

Section: Sensitisation and Antigen Challengementioning

confidence: 99%

“…This immediate reaction, which is complete within 30 min, is followed by a late reaction which consists of a more pro longed infiltration of inflammatory cells, especially neutrophilic polymorphonuclear leucocytes, reaching maximum cell counts at 4-8 h after antigen challenge [12].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Drugs on the Acute Inflammation Following Intraperitoneal Injection of Antigen into Actively Sensitised Rats

Sharpe¹,

Smith²

1979

Int Arch Allergy Immunol

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When actively sensitised rats were injected intraperitoneally with antigen, the local reaction that ensued can be divided into two phases: an immediate reaction characterised by histamine and SRS-A release with an associated extravasation of plasma proteins, and a late reaction involving infiltration of neutrophilic polymorphonuclear leucocytes. When the immediate reaction was modified by BRL 10833 (which inhibits histamine release from rat mast cells and reduces extravasation of plasma proteins), there was no reduction in neutrophil infiltration. FPL 55712, an SRS-A antagonist, also failed to inhibit neutrophil infiltration. The β-adrenoreceptor stimulants isoprenaline and salbutamol reduced neutrophil infiltration. Isoprenaline inhibited the extravasation of plasma proteins when given before antigen, but even when administered to rats after antigen, when extravasation was complete, it still inhibited neutrophil infiltration. Propranolol reversed isoprenaline-induced inhibition of neutrophil infiltration.

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Section: Discussionmentioning

confidence: 99%

Section: Sensitisation and Antigen Challengementioning

confidence: 99%

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Effects of Drugs on the Acute Inflammation Following Intraperitoneal Injection of Antigen into Actively Sensitised Rats

Sharpe¹,

Smith²

1979

Int Arch Allergy Immunol

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“…The former is believed to be established by vasoactive/bronchoactive mediators with a subsequent increase in the vas cular permeability and airway resistance [2], The latter is characterized by an intense cellular infiltration, mainly eo sinophils [3]. Since the phenomenon of chemotaxis is one of the proposed mechanisms by which eosinophils may ac cumulate in a tissue [4], some endogenous eosinophil chemotaxins such as eosinophil chemotactic factor of anaphy laxis [5] and mediators such as C5a [6], leukotriene B4 (LTB4) [7] -preferentially PAF-acether [8] -are potential contributors to tissue eosinophil infiltration.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Modulation of the Late Eosinophilia Induced by Antigen in Actively Sensitized Rats

Silva

Martins²,

Lima³

et al. 1992

Int Arch Allergy Immunol

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The intrathoracic injection of ovalbumin (12 μg/cavity) into actively sensitized rats led to a long-lasting eosinophil recruitment, which appeared 24 h after stimulation. In this study, pharmacological antagonists were used in order to evaluate the potential involvement of arachidonic acid metabolites and PAF-acether in the pleural eosinophil accumulation by antigen. Administration of the cyclooxygenase inhibitor indomethacin (2 mg/kg, i.p.), 1 h before the antigen challenge, failed to modify the 24-hour eosinophilia. In contrast, the dual cyclooxygenase and lipoxygenase inhibitor BW 755C and the more selective inhibitor BW A4C (5 and 10 μg/cavity, i.t.), injected 1 h before the antigen, were effective. Similarly, the PAF-acether antagonists BN 52021 and WEB 2086 (20 mg/kg, i.p.) abrogated the eosinophil accumulation, which was also sensitive to the topical treatment with the glucocorticoid dexamethasone (5 and 10 μg/ cavity). Our findings suggest that the antigen-induced eosinophil mobilization is dependent on lipoxygenase derivatives and PAF-acether, but not on prostaglandins.

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The effects of drugs on leucocyte changes following the injection of antigen into the peritoneal cavities of actively sensitised rats

Spicer¹,

Laycock²,

Smith³

1986

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The injection of antigen into the peritoneal cavities of actively sensitised rats produced an immediate reaction characterised by an increase in concentrations in the peritoneal fluids, collected 5 min later, of extravasated dye labelled plasma proteins, histamine and slow reacting substance of anaphylaxis (SRS-A). Changes were also produced in the numbers of leucocytes in the blood and peritoneal cavity. 5 min after antigen challenge there was a reduction in the number of cells that could be washed from the peritoneal cavity. 4 h after antigen there was an increase in numbers of neutrophils both in the blood and peritoneal washings and these fell to the levels in control rats at 24 h. 24 h after antigen, and continuing for 72 h, there was an increase in numbers of eosinophils and mononuclear cells in the peritoneal washings. The rats were injected intravenously with sephadex particles to produce a blood eosinophilia at the time of antigen challenge, this increased the numbers of eosinophils migrating into the peritoneal cavity but had no effect on antibody levels, the numbers of other leucocytes or on the immediate reaction. An inhibitor of lipoxygenase and cyclo-oxygenase metabolism of arachidonic acid, phenidone, at 100 mg/kg p.o., inhibited SRS-A release to control levels, in the immediate reaction, but had no effect on the leucocyte changes. The glucocorticosteroid, dexamethasone, at doses of 0.1 and 1 mg/kg p.o., produced little inhibition of SRS-A release but significantly inhibited neutrophil, eosinophil and mononuclear cell infiltration into the peritoneal cavity.(ABSTRACT TRUNCATED AT 250 WORDS)

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Acute Inflammation Following Intraperitoneal Injection of Antigen into Actively Sensitised Rats

Cited by 7 publications

References 11 publications

Effects of Drugs on the Acute Inflammation Following Intraperitoneal Injection of Antigen into Actively Sensitised Rats

Effects of Drugs on the Acute Inflammation Following Intraperitoneal Injection of Antigen into Actively Sensitised Rats

Pharmacological Modulation of the Late Eosinophilia Induced by Antigen in Actively Sensitized Rats

The effects of drugs on leucocyte changes following the injection of antigen into the peritoneal cavities of actively sensitised rats

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