Acute Impacts of Different Types of Exercise on Circulating α-Klotho Protein Levels

Abstract

Introduction: Elevated plasma α-klotho (αKl) protects against several ageing phenotypes and has been proposed as a biomarker of a good prognosis for different diseases. The beneficial health effects of elevated plasma levels of soluble αKl (SαKl) have been likened to the positive effects of exercise on ageing and chronic disease progression. It has also been established that molecular responses and adaptations differ according to exercise dose. The aim of this study is to compare the acute SαKl response to different exercise interventions, cardiorespiratory, and strength exercise in healthy, physically active men and to examine the behavior of SαKl 72h after acute strength exercise.Methods: In this quasi-experimental study, plasma SαKl was measured before and after a cardiorespiratory exercise session (CR) in 43 men, and strength exercise session (ST) in 39 men. The behavior of SαKl was also examined 24, 48, and 72h after ST.Results: Significant differences (time×group) were detected in SαKl levels (p=0.001; d=0.86) between CR and ST. After the ST intervention, SαKl behavior varied significantly (p=0.009; d=0.663) in that levels dropped between pre- and post-exercises (p=0.025; d=0.756) and were also significantly higher compared to pre ST values at 24h (p=0.033; d=0.717) and at 48h (p=0.015; d=0.827).Conclusions: SαKl levels increased in response to a single bout of cardiorespiratory exercise; while they decreased immediately after strength exercise, levels were elevated after 24h indicating different klotho protein responses to different forms of exercise.

Effect of combined aerobic and resistance exercise on serum Klotho secretion in healthy young men -a pilot study-

“…We recently demonstrated that acute high-intensity resistance exercise increases serum KL concentrations in healthy young men ( Morishima and Ochi, 2021 ). On the other hand, a previous study reported a significant reduction of plasma KL concentrations immediately after acute jump exercise ( Iturriaga et al, 2021 ). Although these studies were conducted with the understanding of the influence of aerobic and resistance exercise on the KL response, an important limitation is that no prior study examined the impact of combined aerobic and resistance exercise on blood KL secretion.…”

Study in Parkinson’s disease of exercise phase 3 (SPARX3): study protocol for a randomized controlled trial

“…We are assaying klotho which is an aging regulator that when overexpressed, extends life in model organisms and augments cognition [ 97 , 98 , 99 ]. While klotho levels decrease with aging and PD [ 100 ], physical exercise robustly increases klotho levels in healthy adults [ 101 , 102 ]. Similarly, recent data show glycosylphosphatidylinositol-specific phospholipase D1 (GPLD1) is higher in older people than those who are more active (takes greater than 7100 steps daily) than in those who are less active (takes less than 7100 steps daily).…”

αKlotho decreases after reduced weight-bearing from both spaceflight and hindlimb unloading

“…Changes in α-Klotho after acute bouts of exercise are inconsistent when examining resistance vs aerobic exercise (ref. 16 ), with long term exercise exhibiting elevated αKlotho in sedentary individuals (ref. 17 ).…”