Acute harmine administration induces antidepressive-like effects and increases BDNF levels in the rat hippocampus

Fortunato, Jucélia Jeremias; Réus, Gislaine Z.; Kirsch, Tamires R.; Stringari, Roberto B.; Stertz, Laura; Kapczinski, Flávio; Pinto, José Roberto Aparecido dos Santos; Hallak, Jaime Eduardo Cecílio; Zuardi, Antônio Waldo; Crippa, José Alexandre de Souza; Quevedo, João

doi:10.1016/j.pnpbp.2009.07.021

Cited by 117 publications

(118 citation statements)

References 36 publications

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“…Effect of harmine on animal behavior was assessed in the forced swimming and open-field tests, and the results showed harmine reduced immobility time and increased both climbing and swimming time of rats, compared to saline group. Harmine at a higher dose level also increased brain-derived neurotrophic factor protein levels in the rat hippocampus [23] . Effects of harmine on apomorphine-induced pecking behavior in chicks were also investigated.…”

Section: Effect Of Harmine On Central Nervous Systemmentioning

confidence: 88%

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

Patel

Gadewar

Tripathi

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

214

120

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Section: Effect Of Harmine On Central Nervous Systemmentioning

confidence: 88%

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

Patel

Gadewar

Tripathi

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

214

120

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“…9) Harmine is also found in common plant-derived foods and animal tissues, 10) and it has antiplasmodial, antioxidative, antimutagenic and antigenotoxic activities, as well as antidepressant properties. [11][12][13] Several psychiatric conditions, including depression, are closely associated with the circadian clock. 14) Although we previously reported that harmine dose-dependently lengthens the circadian period of Bmal1 expression in NIH 3T3 cells, 15) the effects of harmine on the master clock in the SCN remain unknown.…”

mentioning

confidence: 99%

Harmine Lengthens Circadian Period of the Mammalian Molecular Clock in the Suprachiasmatic Nucleus

Kondoh

Yamamoto

Tomita

et al. 2014

Biological & Pharmaceutical Bulletin

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The circadian clock is a cell-autonomous endogenous system that generates circadian rhythms in the behavior and physiology of most organisms. We previously reported that the harmala alkaloid, harmine, lengthens the circadian period of Bmal1 transcription in NIH 3T3 fibroblasts. Clock protein dynamics were examined using real-time reporter assays of PER2::LUC to determine the effects of harmine on the central Key words carboline alkaloid; circadian clock; PER2::LUC protein; real-time reporter assay; suprachiasmatic nucleus The circadian clock is an endogenous cell-autonomous system that generates circadian rhythms in the behavior and physiology of most organisms. The master clock in mammals is located in the suprachiasmatic nucleus (SCN) of the anterior hypothalamus, 1,2) which controls most aspects of physiology such as sleep-wake cycles, body temperature, hormone secretion, blood pressure and metabolism.3,4) The circadian clock mechanism consists of a network of autoregulatory transcription-based feedback loops that drive the rhythmic expression of clock genes such as Per1, Per2 and Bmal1, 5) and these cell-autonomous and self-sustained oscillators are found not only in the SCN but also in peripheral tissues as well as dissociated cells. Acute induction of the mRNA expression of clock genes plays a critical role in phase shifting the circadian clock both in cells cultured in vitro and in vivo. In addition to transcriptional feedback regulations, some essential roles of post-translational modifications such as phosphorylation and ubiquitination have been reported. [6][7][8] The harmala alkaloid, harmine, is a β-carboline alkaloid that was originally isolated from seeds of Peganum harmala and Banisteropsis caapi, both of which have traditionally been used in ritual and medicinal preparations of the Middle East, Central Asia and South America. 9) Harmine is also found in common plant-derived foods and animal tissues, 10) and it has antiplasmodial, antioxidative, antimutagenic and antigenotoxic activities, as well as antidepressant properties.11-13) Several psychiatric conditions, including depression, are closely associated with the circadian clock.14) Although we previously reported that harmine dose-dependently lengthens the circadian period of Bmal1 expression in NIH 3T3 cells, 15) the effects of harmine on the master clock in the SCN remain unknown. We therefore evaluated clock protein dynamics in neuronal cells and in SCN slices by monitoring PER2::LUC bioluminescence in real-time to determine the effects of harmine on the master clock in the SCN.

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“…Despite the significant increase in extracellular of DA levels induced by harmine, it had no changes in locomotor activity [46] in the OFT [47]. While it is possible that the MAO-A inhibition of harmine has some functional effect on DA efflux in the striatum in vivo.…”

Section: Discussionmentioning

confidence: 90%

ESTIMATION OF THE ANXIOLYTIC-LIKE EFFECT OF THE Β-Carboline ALKALOID HARMINE ON STRESSED PREGNANT RATS

Benatoui

Bairi

Tahraoui

2017

Int J Pharm Pharm Sci

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Objective: During the last decade, the role of the β-carboline alkaloid harmine has essentially been studied with regard to its anxiolytic effect, as it was done in our laboratory; therefore, this study has been progressed to cover the effect of this alkaloid on pregnant wistar rats. Methods:The molecule was used at doses of 10 mg/kg, 15 mg/kg, pregnant female rats were divided into three groups according to the stage of pregnancy: first, second, and the third week of pregnancy. Each group has been subdivided into seven subgroups: control group, two treated groups with harmine, acute footshock stress at 1,2mA, sub-acute footshock stress at 0,4mA, psychological stress, and the treated group that footshocked after with 1,2mA, all groups were carried out open field test, plus maze test and light/dark box test. Results:Thigmotaxis is reflected by the significant increase in the traveled distance in peripheral area in the open field of the three groups 'weeks' at dose of 10 mg/kg, the enhancement in the number and time of rearing, at both doses, during the second and the last week, the significant increase in the number of entries 'in open arms' in plus-maze during the first and third weeks at 15 mg/kg, and the significant decreased in time spent in the light compartment of the light/dark box at the same dose of all groups 'weeks' were noticed, which confirm the anxiolytic effect of the alkaloid, even in the case of the footschock stressed pregnant rats of all groups 'weeks' that enhancement of number of enties into open arms during the plus maze test. Conclusion:So we can conclude that the anxiolytic effect of harmine not shortening to male rats, but expands to female pregnant wistar rats, and establishes its effect by diminishing time in light compartment of light/dark box and number of entries in open arms of plus maze, in other hand, the increase in the number and the time of rearing reflects the enhancement of exploratory behavior.

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Acute harmine administration induces antidepressive-like effects and increases BDNF levels in the rat hippocampus

Cited by 117 publications

References 36 publications

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

Harmine Lengthens Circadian Period of the Mammalian Molecular Clock in the Suprachiasmatic Nucleus

ESTIMATION OF THE ANXIOLYTIC-LIKE EFFECT OF THE Β-Carboline ALKALOID HARMINE ON STRESSED PREGNANT RATS

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