Summary:There is increasing evidence that the immune response plays a role in the prevention of leukemic relapses after allogeneic bone marrow transplantation (BMT). Producing this effect (referred to as the graft-versus-leukemia reaction or GVL) is a current goal of clinical transplantation. At present, all protocols rely on the injection of donor T cells with unknown specificities. In keeping with this approach, we recently proposed the use of a single allogeneic T cell clone transfected with the HSvtk gene to target an HLA-DPB1 mismatch in the GVH direction. For this strategy to be successful, HLA-DP antigens must be expressed on leukemic cells, which should be recognised by the HLA-DP-specific T cell clone and subsequently destroyed. In the present study, differential expression of HLA-DR, -DQ and -DP was tested by fluorescence using monoclonal antibodies on a panel of 46 acute myeloid leukemias (AML), 28 acute lymphoblastic leukemias (ALL) and 31 chronic lymphocytic leukemias of B cell origin (B-CLL). The vast majority of leukemic cells expressed HLA-DP antigens although with considerable variability. HLA-DPB1 genotyped leukemic cells were used as target cells for an HLA-DPB1*0401-specific T cell clone. Specific recognition of leukemic blasts was demonstrated for 11 out of 11 B-CLL, 11 out of 19 AML and nine out of 16 ALL. These data show that most leukemic blasts are accessible to direct lysis by allogeneic HLA-DP-specific T cells. Keywords: immunotherapy; BMT; GVL; CTL; HLA-DP Clinical studies have demonstrated that alloreactivity after bone marrow transplantation (BMT) is responsible not only for graft-versus-host disease (GVHD) but also for a beneficial effect referred to as graft-versus-leukemia reaction (GVL). Evidence for GVL comes from observations showing the balance between the two aspects of the allogeneic reaction, ie patients who experienced GVHD had fewer relapses than those without GVHD, whereas only those with mild GVHD had improved survival. 1-3 GVHD and Correspondence: Dr H Vié, INSERM U463, Institut de Biologie, 9 Quai Moncousu, 44035 Nantes Cedex, France Received 5 October 1998; accepted 13 January 1999 GVL are both mediated by donor T lymphocytes, but it is still not possible to determine the subsets responsible for one or the other. Accordingly, most recent efforts to control alloreaction (ie to keep the benefit of GVL while avoiding the mortality and morbidity due to GVHD) have focused on transplanted T cells as a whole. For example, some groups have considered the injection of graded doses of donor T cells either as a preventive regimen 4-6 or in the case of relapse. 7-13 More recently, it was suggested that donor T lymphocytes be transduced with the herpes simplex thymidine kinase gene (HSv-tk) before grafting 14 to facilitate their destruction through ganciclovir treatment in the event that GVHD occurs. In the above protocols, whole donor T lymphocyte populations were injected into the recipient. Consequently, neither the relative importance of different HLA target antigens nor the...