Acute exposure to organic and inorganic sources of copper: Differential response in intestinal cell lines

Keenan, Joanne; O’Sullivan, Finbarr; Henry, Michael; Breen, Laura; Doolan, Padraig; Sinkunaite, Indre; Clynes, Martin; Horgan, Karina; Murphy, Richard

doi:10.1002/fsn3.857

Cited by 7 publications

(4 citation statements)

References 45 publications

(59 reference statements)

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“…Gut microbiota has been widely recognized to influence the digestion and absorption of nutrients by the host, and it plays an important role in maintaining host immune response and healthy growth (Keenan et al, ; Valdes, Walter, Segal, & Spector, ). The gut microbiota is easily influenced by the changes in the microbial milieu and health status of the host and closely related to the growth stage and daily dietary intake of the host.…”

Section: Long‐term Supplementation Of High Copper Diet and Intestinalmentioning

confidence: 99%

Advances in the mechanism of high copper diets in restraining pigs growth

Gao

Yang

Che

et al. 2019

Animal Physiology Nutrition

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High copper feed has been widely used as an inexpensive and highly effective feed additive to promote growth performance of pigs. However, long‐term feeding of high copper feed may reduce the growth‐promoting effects of copper, time‐dependent accumulation of copper in animal tissues and organs, and copper toxicity thereby reducing the growth performance of pigs. Due to the widespread effects of high copper supplementation in animals' diets, the benefits and drawbacks of high copper feeding in pigs have been reported in several studies. Meanwhile, few of these studies have systematically described the mechanism by which high copper diets restrain pig growth. Therefore, to address the concerns and give a better understanding of the mechanism of high copper diet in restraining pig growth in different systems, this paper reviews the research progress of long‐term supplementation of high copper on the growth of pigs and provides some suggestions and further research directions.

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Section: Long‐term Supplementation Of High Copper Diet and Intestinalmentioning

confidence: 99%

Advances in the mechanism of high copper diets in restraining pigs growth

Gao

Yang

Che

et al. 2019

Animal Physiology Nutrition

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“…Joanne Keenan et al compared organic and inorganic copper exposure to HT29 and Caco-2 cells. Their findings showed that PepT1, ZnT1, and CTR1 were increased, particularly for CuSO 4 and copper proteinate in Caco-2 cells [17].…”

Section: Introductionmentioning

confidence: 95%

Different Sources of Copper Effect on Intestinal Epithelial Cell: Toxicity, Oxidative Stress, and Metabolism

Wen

Lin

et al. 2019

Metabolites

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Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. However, high doses of copper will induce cell damage and toxicity. The aim of this study was to evaluate toxicity, bioavailability, and effects on metabolic processes of varying copper sources using porcine intestinal epithelial cells (IPEC-J2) as a model. The IPEC-J2 were treated with two doses (30 and 120 μM) of CuSO4, Cu Glycine (Cu-Gly), and Cu proteinate (Cu-Pro) for 10 h, respectively. Cell damage and cellular copper metabolism were measured by the changes in cell viability, copper uptake, oxidative stress biomarkers, and gene/protein expression levels. The results showed that cell viability and ratio of reduced and oxidized glutathione (GSH/GSSG) decreased significantly in all treatment groups; intracellular copper content increased significantly in all treatment groups; total superoxide dismutase (SOD) activity increased significantly in the 120 μM exposed groups; SOD1 protein expression levels were significantly upregulated in 30 μM Cu-Pro, 120 μM Cu-Gly, and 120 μM Cu-Pro treatment groups; intracellular reactive oxygen species (ROS) generation and malondialdehyde (MDA) content increased significantly in 30 μM treatment groups and 120 μM CuSO4 treatment group. CTR1 and ATP7A gene expression were significantly downregulated in the 120 μM exposed groups. While upregulation of ATOX1 expression was observed in the presence of 120 μM Cu-Gly and Cu-Pro. ASCT2 gene expression was significantly upregulated after 120 μM Cu-Glycine and CuSO4 exposure, and PepT1 gene expression was significantly upregulated after Cu-Pro exposure. In addition, CTR1 protein expression level decreased after 120 μM CuSO4 and Cu-Gly exposure. PepT1 protein expression level was only upregulated after 120 μM Cu-Pro exposure. These findings indicated that extra copper supplementation can induce intestinal epithelial cell injury, and different forms of copper may have differing effects on cell metabolism.

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“…The effect of intestinal microbiota on nutrient uptake and maintaining healthy growth is broadly known ( Keenan et al., 2018 ). The gut microbiota changes that occur are related to animal production phase and feed composition.…”

Section: Introductionmentioning

confidence: 99%

Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

Wen

Piao

et al. 2022

Animal Nutrition

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Copper (Cu) is an essential trace element in the production of swine. This study was conducted to investigate the effect of 3 different sources of Cu on growth performance, Cu metabolism, and intestinal microorganisms of finishing pigs, so as to estimate the bioavailability of the 3 sources for pigs. A total of 42 male finishing pigs (88.74 ± 5.74 kg) were randomly allocated to 7 treatments. The factors were 3 sources (CuSO 4 , Cu-glycine, Cu-proteinate) and 2 levels (5 and 20 mg/kg) of Cu, plus one negative control treatment (0 mg/kg added Cu level) for the entire 28-d experiment. The average daily gain (ADG) and feed to gain ratio (F:G) both increased when Cu was added. The Cu level in liver, bile, kidney, serum, lung, urine and feces rose ( P < 0.001) with increasing dietary Cu level regardless of the source. Meanwhile, pigs receiving organic Cu (glycinate or proteinate) retained more Cu and excreted less Cu than those receiving inorganic Cu (CuSO 4 ), which showed that organic forms were more bioavailable. At the transcriptional level, changes in the level and source of dietary Cu resulted in modulation of transporters. In the jejunal mucosa, import transporter high affinity copper uptake protein 1 ( CTR1 ) and export transporter ATPase copper transporting alpha ( ATP7A ) in supplemental Cu treatments were down-regulated compared to the control. Also, peptide transporter 1 ( PepT1 ) and lanine-serine-cysteine transporter, type-2 ( ASCT2 ) were significantly ( P < 0.01) up-regulated in 20 mg/kg Cu-proteinate and Cu-glycinate treatments, respectively. Microbial diversity was lowest in the 20 mg/kg CuSO 4 treatment, and the ratio of Firmicutes to Bacteroidetes was higher in added Cu treatments, especially Cu-glycinate treatment. These results indicate that uptake of different Cu forms is facilitated by different transporters and transport mechanisms, and compared with inorganic Cu, organic Cu provides benefits to intestinal microflora and reduces Cu excretion.

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Acute exposure to organic and inorganic sources of copper: Differential response in intestinal cell lines

Cited by 7 publications

References 45 publications

Advances in the mechanism of high copper diets in restraining pigs growth

Advances in the mechanism of high copper diets in restraining pigs growth

Different Sources of Copper Effect on Intestinal Epithelial Cell: Toxicity, Oxidative Stress, and Metabolism

Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

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