Acute exposure of mercury chloride stimulates the tissue regeneration program and reactive oxygen species production in the Drosophila midgut

Chen, Zhi; Wu, Xiaochun; Luo, Hongjie; Zhao, Lingling; Ji, Xin; Qiao, Xianfeng; Jin, Yaping; Liu, Wei

doi:10.1016/j.etap.2015.11.009

Cited by 16 publications

(9 citation statements)

References 23 publications

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“…In these animals, ROS are detected at the level of the amputation plane shortly after amputation, and reduction of H 2 O 2 levels impairs regeneration [ 164 , 180 ] ( Figure 3 B). In Drosophila , regeneration can be observed in imaginal discs during larval development and adult gut regeneration [ 181 , 182 , 183 ]. In both systems, regeneration is redox-dependent [ 153 , 181 , 182 , 184 , 185 , 186 , 187 ].…”

Section: Redox Signalling In Animal Development and Regenerationmentioning

confidence: 99%

Hydrogen Peroxide and Redox Regulation of Developments

et al. 2018

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Reactive oxygen species (ROS), which were originally classified as exclusively deleterious compounds, have gained increasing interest in the recent years given their action as bona fide signalling molecules. The main target of ROS action is the reversible oxidation of cysteines, leading to the formation of disulfide bonds, which modulate protein conformation and activity. ROS, endowed with signalling properties, are mainly produced by NADPH oxidases (NOXs) at the plasma membrane, but their action also involves a complex machinery of multiple redox-sensitive protein families that differ in their subcellular localization and their activity. Given that the levels and distribution of ROS are highly dynamic, in part due to their limited stability, the development of various fluorescent ROS sensors, some of which are quantitative (ratiometric), represents a clear breakthrough in the field and have been adapted to both ex vivo and in vivo applications. The physiological implication of ROS signalling will be presented mainly in the frame of morphogenetic processes, embryogenesis, regeneration, and stem cell differentiation. Gain and loss of function, as well as pharmacological strategies, have demonstrated the wide but specific requirement of ROS signalling at multiple stages of these processes and its intricate relationship with other well-known signalling pathways.

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Section: Redox Signalling In Animal Development and Regenerationmentioning

confidence: 99%

Hydrogen Peroxide and Redox Regulation of Developments

et al. 2018

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“…Stress through the studied microbiota and chemicals such as bleomycin and paraquat converge on EC damage and consequent loss, which acts as a trigger to induce ISC proliferation through the same regenerative pathways. Inorganic mercury (HgCl 2 ) has a similar effect on ISC proliferation through damage to EC, although its effect on signaling pathways has not been studied yet (Chen et al, 2015).…”

Section: Are Stresses Converging Through the Same Mechanisms?mentioning

confidence: 99%

From pathogens to microbiota: How Drosophila intestinal stem cells react to gut microbes

Bonfini¹,

Liu²,

Buchon³

2016

Developmental & Comparative Immunology

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The intestine acts as one of the interfaces between an organism and its external environment. As the primary digestive organ, it is constantly exposed to a multitude of stresses as it processes and absorbs nutrients. Among these is the recurring damage induced by ingested pathogenic and commensal microorganisms. Both the bacterial activity and immune response itself can result in the loss of epithelial cells, which subsequently requires replacement. In the Drosophila midgut, this regenerative role is fulfilled by intestinal stem cells (ISCs). Microbes not only trigger cell loss and replacement, but also modify intestinal and whole organism physiology, thus modulating ISC activity. Regulation of ISCs is integrated through a complex network of signaling pathways initiated by other gut cell populations, including enterocytes, enteroblasts, enteroendocrine and visceral muscles cells. The gut also receives signals from circulating immune cells, the hemocytes, to properly respond against infection. This review summarizes the types of gut microbes found in Drosophila, mechanisms for their elimination, and provides an integrated view of the signaling pathways that regulate tissue renewal in the midgut.

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“…According to previous studies, 50 µg/mL BSP and 400 µM HgCl 2 were used in the present study (Zhang et al 2015;Yue et al 2016;Chen et al 2016). Both HgCl 2 (Sigma Aldrich) and BSP (XI'AN ZeiLan) was diluted with 5% sucrose.…”

Section: Bsp Treatmentmentioning

confidence: 99%

“…Exposure of Drosophila to mercury chloride resulted in inhibition of antioxidant enzyme activity, reactive oxygen species (ROS) overproduction, lipid peroxidation, reduction of fertility and even alteration of somatic and germline cells (Paula et al 2014;Mojica-Vázquez et al 2019). Further study suggested that mercury exposure caused severe oxidative damage to midgut epithelium and disrupted the homeostasis of intestinal stem cells (ISCs) in midguts (Chen et al 2016;Chen et al 2021). Therefore, the exploration of new, rich sources of natural antioxidants has become the top priority to prevent and treat various oxidative stress-related diseases including hydrargyrism.…”

Section: Introductionmentioning

confidence: 99%

Bletilla striata polysaccharides protect against mercury-induced oxidative damage to Drosophila midguts via modulation of sestrin

Chen

Wen

2022

Preprint

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Oxidative stress was one of the major causes of heavy metal-induced toxicity in organisms including Drosophila melanogaster. Bletilla striata polysaccharide (BSP) with anti-oxidative property has been recently recognized as a novel player in the management of oxidative stress response in organisms. Here, we took Drosophila midgut as a model to evaluate the protective effects of BSP (50 µg/mL) on mercury chloride-induced gastrointestinal adversities. As a result, BSP was found to significantly improve the survival rates and climbing ability of flies exposed to mercury. Further study demonstrated that BSP significantly alleviated the mercury-induced injury to midgut epithelium, at least partly, through increasing antioxidant enzyme activity (glutathione-S-transferase and superoxide dismutase), decreasing reactive oxidative species production, inhibiting cell apoptosis, restoring intestinal epithelial barrier and regulating stem cell-mediated tissue regeneration. Additionally, oxidative stress responsive gene sestrin was involved in the protection of BSP against mercury-induced oxidative damage to midguts. This study suggested that BSP has great potential for future application in the treatment and prevention of heavy metal-induced gastrointestinal adversities in mammals.

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Acute exposure of mercury chloride stimulates the tissue regeneration program and reactive oxygen species production in the Drosophila midgut

Cited by 16 publications

References 23 publications

Hydrogen Peroxide and Redox Regulation of Developments

Hydrogen Peroxide and Redox Regulation of Developments

From pathogens to microbiota: How Drosophila intestinal stem cells react to gut microbes

Bletilla striata polysaccharides protect against mercury-induced oxidative damage to Drosophila midguts via modulation of sestrin

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