Acute Experimental Diabetes and Aortic Depressor Nerve Ultrastructural Morphometry: Effects of Insulin Treatment

Oliveira, Fabrício Singaretti de

doi:10.4172/2161-0940.1000159

Cited by 2 publications

(2 citation statements)

References 31 publications

(40 reference statements)

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“…The experimental model used in this study has been used in our laboratory for decades [19,[23][24][25][26][27] and has proven to be a good experimental model of acute and chronic diabetes mellitus, not only for describing the morphological alterations on several peripheral nerves due to diabetes mellitus [16], but also to investigate physiological alterations on nerve function and cardiovascular regulation deficits in diabetes mellitus [26,27].…”

Section: Discussionmentioning

confidence: 99%

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Myelin Area and Axon Diameter Relation in Renal Nerve Myelinated Fibers of Acute and Chronic Diabetic Rats

VPS¹,

KL²

2018

IJAAP

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The positive relation between the axon size and its respective myelin sheath has long been known. We tested the hypothesis that the expected correlation between myelin sheath area (My a ) and the axonal diameter (D ax ) of myelinated fibers in the renal nerves is altered by diabetes, using a regression analysis study. Male Wistar rats were used after 15 days or 12 weeks of diabetes induction by a single injection of streptozotocin (STZ i.v.). Control rats received vehicle. Insulin-treated animals received a single subcutaneous injection of insulin on a daily basis. Then, the left renal nerves were dissected, removed and prepared for light microscopy. Morphometry of the myelinated fibers was carried out with computer software. The My a and the D ax of all myelinated fibers in each nerve were automatically measured. Regression lines were calculated for all nerves in each group. Differences between the regression lines were tested for slope and intercept as well as differences between the correlation coefficients. The regression lines were coincident between the three acute groups. Also, there was a coincidence between the regression lines from control and insulin-treated chronic groups. The chronic diabetic group showed a significant slope reduction on the regression lines, as well as a reduced correlation coefficient when compared to all other experimental groups. These results show that there are morphological alterations on the renal nerve myelinated fibers in STZ induced diabetes consisting of axonal atrophy and/or demyelinating-remyelinating processes. These results may be useful in the understanding of the renal dysfunctions found in diabetic nephropathy.

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Section: Discussionmentioning

confidence: 99%

“…The animals were considered diabetic when the blood glucose levels were higher than 350 mg/dl. Details of this experimental model of diabetes induction and insulin treatment were previously described [10,[23][24][25][26][27].…”

Section: Experimental Groupsmentioning

confidence: 99%

Myelin Area and Axon Diameter Relation in Renal Nerve Myelinated Fibers of Acute and Chronic Diabetic Rats

VPS¹,

KL²

2018

IJAAP

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Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study

Sanada

Tavares

Sato

et al. 2015

Diabetol Metab Syndr

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BackgroundProspective studies have shown incidence rates of hypertension in diabetes mellitus to be three times that of subjects without diabetes mellitus. The reverse also applies, with the incidence of diabetes two to three times higher in patients with hypertension. Despite this common clinical association, the contribution of each isolated entity in the development of a neuropathy is still not well understood. The aims of the present study were to investigate the presence of peripheral neuropathy in spontaneously hypertensive rats (SHR) and SHR with chronically induced diabetes, using a morphological and morphometric study of the sural nerves.MethodsFemale SHR and normotensive Wistar rats (WR), 8 weeks old, received a single intravenous injection of streptozotocin (STZ) through the tail vein. Controls from both strains received vehicle. Twelve weeks after the injection, sural nerves were dissected and prepared for light microscopy. Morphometry of sural nerve fascicles and myelinated fibers was performed with the aid of computer software.ResultsThe sural nerve myelinated fibers were highly affected by experimental diabetes in normotensive rats, causing mainly the reduction of the fiber size. Hypertensive rats showed characteristics of small fiber neuropathy and a severe reduction of the number and density or Schwann cells. The association between diabetes and hypertension caused an increase on the average size of the myelinated fibers, pointing to a small fiber loss, associated to axonal atrophy.ConclusionsOur study gives morphological support to the existence of a neuropathy due to hypertension, which is among one of the most common risk factors for diabetic neuropathy. The association between the two neuropathies showed to be a complex alteration, involving and including both, large and small fibers neuropathy. Hypertension caused, indeed, an exacerbation of the alterations already observed in experimental models of diabetic neuropathy.

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Acute Experimental Diabetes and Aortic Depressor Nerve Ultrastructural Morphometry: Effects of Insulin Treatment

Cited by 2 publications

References 31 publications

Myelin Area and Axon Diameter Relation in Renal Nerve Myelinated Fibers of Acute and Chronic Diabetic Rats

Myelin Area and Axon Diameter Relation in Renal Nerve Myelinated Fibers of Acute and Chronic Diabetic Rats

Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study

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