Acute endothelial cell contraction in vitro: A comparison with vascular smooth muscle cells and fibroblasts

Boswell, Carl A.; Majno, Guido; Joris, Isabelle; Ostrom, Kristen A.

doi:10.1016/0026-2862(92)90015-h

Cited by 48 publications

(21 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether a decrease in the level ofMLC20 phosphorylation enhances endothelial barrier function is unclear. Using silicon rubber matrix, other investigators have shown that increasing cell cAMP induces matrix relaxation (31,32). If centripetal isometric tension opposes tethering forces that link cells and substrate together, then, perhaps, a decrease in MLC20 phosphorylation could decrease opposing tension on tethering forces which, in turn, could enhance adhesive forces between adjacent cells and cells to substrate.…”

Section: Discussionmentioning

confidence: 99%

The effect of histamine and cyclic adenosine monophosphate on myosin light chain phosphorylation in human umbilical vein endothelial cells.

Moy¹,

Shasby²,

Scott³

et al. 1993

J. Clin. Invest.

118

View full text Add to dashboard Cite

Histamine causes adjacent endothelial cells to retract from each another. We examined phosphorylation of the 20-kD myosin light chain (MLC20) in human umbilical vein endothelial cells (HUVECs) exposed to histamine to determine if we could find evidence to support the hypothesis that retraction of these cells in response to histamine represents an actomyosininitiated contraction of the endothelial cytoskeleton. We found that MLC20 in HUVECs was constitutively phosphorylated

show abstract

Section: Discussionmentioning

confidence: 99%

The effect of histamine and cyclic adenosine monophosphate on myosin light chain phosphorylation in human umbilical vein endothelial cells.

Moy¹,

Shasby²,

Scott³

et al. 1993

J. Clin. Invest.

118

View full text Add to dashboard Cite

show abstract

“…Contraction was measured as reduction in lattice area over time and is shown 24 h after release. Investigation of cellular contraction using collagen lattices (gels) was introduced by Bell and colleagues and has been validated as a model for study of cellular contraction in a variety of mesenchymal cell types (30,(39)(40)(41)(42)(43)(44). For example, fibroblasts seeded on top oftype I collagen gels bind and organize underlying collagen fibrils, both in close proximity and at distance from cells (39).…”

Section: <0001mentioning

confidence: 99%

Activation-dependent contractility of rat hepatic lipocytes in culture and in vivo.

Rockey

Housset²,

Friedman³

1993

J. Clin. Invest.

258

193

View full text Add to dashboard Cite

As a test of the in vivo relevance of the culture findings, lipocytes were isolated from fibrotic animals and examined immediately after adherence. Whereas lipocytes from normal liver were initially compact, smooth muscle a actin negative and noncontractile, cells from animals with hepatic injury due to CC14 displayed an activated appearance, expressed smooth muscle a actin, and were contractile immediately after adherence. Additionally, IFN-y, an agent which blocks lipocyte activation (1992. Hepatology. 16:776), inhibited lipocyte contraction. The data document that normal (i.e., quiescent) lipocytes are not contractile, but that activation is associated with the development of contractility. These findings suggest that a role for lipocytes in organ contraction or vasoregulation may be confined to injured, not normal liver. (J. Clin. Invest. 1993. 92:1795-1804 Key words: lipocyte contractility -extracellular matrix * smooth muscle * actin * pericyte Portions ofthis work were presented in abstract form at the 1992 meet-

show abstract

“…The endothelium is a principal mediator in many homeostatic (38) and disease processes (39,40) and has been considered a widely influential ''organ'' (41). Moreover, endothelial cells have an active actin͞myosin-based contractile system that also may consume O 2 (42,43). In the blood͞tissue interface, endothelium overlays and interacts with smooth muscle throughout the vasculature with the exception of the capillaries, giving origin to the regulatory mechanism that modulates the vessel diameter and controls tissue perfusion.…”

Section: ϫ9mentioning

confidence: 99%

Microvascular and tissue oxygen gradients in the rat mesentery

Tsai

Friesenecker

Mazzoni

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

212

269

View full text Add to dashboard Cite

One of the most important functions of the blood circulation is O 2 delivery to the tissue. This process occurs primarily in microvessels that also regulate blood f low and are the site of many metabolic processes that require O 2 . We measured the intraluminal and perivascular pO 2 in rat mesenteric arterioles in vivo by using noninvasive phosphorescence quenching microscopy. From these measurements, we calculated the rate at which O 2 diffuses out of microvessels from the blood. The rate of O 2 eff lux and the O 2 gradients found in the immediate vicinity of arterioles indicate the presence of a large O 2 sink at the interface between blood and tissue, a region that includes smooth muscle and endothelium. Mass balance analyses show that the loss of O 2 from the arterioles in this vascular bed primarily is caused by O 2 consumption in the microvascular wall. The high metabolic rate of the vessel wall relative to parenchymal tissue in the rat mesentery suggests that in addition to serving as a conduit for the delivery of O 2 the microvasculature has other functions that require a significant amount of O 2 .

show abstract

Acute endothelial cell contraction in vitro: A comparison with vascular smooth muscle cells and fibroblasts

Cited by 48 publications

References 38 publications

The effect of histamine and cyclic adenosine monophosphate on myosin light chain phosphorylation in human umbilical vein endothelial cells.

The effect of histamine and cyclic adenosine monophosphate on myosin light chain phosphorylation in human umbilical vein endothelial cells.

Activation-dependent contractility of rat hepatic lipocytes in culture and in vivo.

Microvascular and tissue oxygen gradients in the rat mesentery

Contact Info

Product

Resources

About