Acute Effects of Resistance Exercise and the Use of GH or IGF-1 Hormones on Oxidative Stress and Antioxidant Markers in Bodybuilders

Abstract

The aim of this study was to examine the influence of peptide hormone use on oxidative stress and antioxidant responses to a single session of resistance exercise in male bodybuilders. Forty-five male bodybuilders were divided into three groups: bodybuilders using growth hormone for at least 1 year (i.e., 3 to 4 times in the year) (GH-user, n = 15), bodybuilders using insulin-like growth factor-1 for at least 1 year (i.e., 3 to 4 times in the year) (IGF-1-user, n = 15), and peptide hormone-free bodybuilders (Non-user, n = 15). The heavy resistance exercise protocol consisted of five sets with 80% of one repetition maximum for six exercises. Blood samples were obtained pre and post heavy resistance exercise (HRE) in order to evaluate changes in oxidative stress (8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO)) and antioxidant biomarkers (glutathione peroxidase (GPx), catalase (CAT) and glutamate (GLU)) level. All the experimental groups showed increases in MDA (p = 0.038), NO (p = 0.028), GPx (p = 0.012), and GLU (p = 0.003) concentrations after resistance exercise. For 8-OHdG, the Non-user and IGF-1-user groups indicated increases at post-exercise (p = 0.001), without any significant changes in the GH-user group (p = 0.87). In addition, the changes in serum GPx and GLU levels were greater for the GH-user group than the Non-user and IGF-1-user groups (p = 0.001). In conclusion, HRE induced significant increases in 8-OHdG (except to GH-user group), MDA, NO, GPx, and GLU levels with greater changes in GPx and GLU for the GH-user group.

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Intra Articular Ozone Modulates Inflammation and Has Anabolic Effect on Knee Osteoarthritis: IL-6 and IGF-1 as Pro-Inflammatory and Anabolic Biomarkers

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Fish scale rich in functional compounds and peptides: A potential nutraceutical to overcome undernutrition

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