Acute effects of light and darkness on the activity and temperature rhythms of a subterranean rodent, the Anillaco tuco-tuco

Yassumoto, Tamiris I.; Tachinardi, Patricia; Oda, Gisele A.; Valentinuzzi, Verónica Sandra

doi:10.1016/j.physbeh.2019.112645

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…Under DD condition, wheel-running experiment was performed to monitor the rhythm of locomotor activity [23]. It has been reported light and darkness had acute effects on the activity and temperature rhythms of a subterranean rodent, the Anillaco tuco-tuco [24]. Besides, aberrant emotional behaviors and cognition were related to altered light conditions [25].…”

Section: Introductionmentioning

confidence: 99%

Endogenous circadian time genes expressions in the liver of mice under constant darkness

Zhang

et al. 2020

BMC Genomics

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Background: The circadian rhythms regulate physiological functions and metabolism. Circadian Time (CT) is a unit to quantify the rhythm of endogenous circadian clock, independent of light influence. To understand the gene expression changes throughout CT, C57BL/6 J mice were maintained under constant darkness (DD) for 6 weeks, and the liver samples were collected starting at 9:00 AM (CT1), and every 4 h in a 24-h cycle (CT5, CT9, CT13, CT17 and CT21). Total RNA was extracted and subjected to RNA-Seq data (deposited as GSE 133342, L-DD). To compare gene oscillation pattern under normal light-dark condition (LD, GSE114400) and short time (2 days) dark-dark condition (S-DD, GSE70497), these data were retried from GEO database, and the trimmed mean of M-values normalization was used to normalize the three RNA-seq data followed by MetaCycle analysis. Results: Approximate 12.1% of the genes under L-DD exhibited significant rhythmically expression. The top 5 biological processes enriched in L-DD oscillation genes were mRNA processing, aromatic compound catabolic process, mitochondrion organization, heterocycle catabolic process and cellular nitrogen compound mitotic catabolic process. The endogenous circadian rhythms of clock genes, P450 genes and lipid metabolism genes under L-DD were further compared with LD and S-DD. The oscillation patterns were similar but the period and amplitude of those oscillation genes were slightly altered. RT-qPCR confirmed the selected RNA sequence findings. Conclusions: This is the first study to profile oscillation gene expressions under L-DD. Our data indicate that clock genes, P450 genes and lipid metabolism genes expressed rhythmically under L-DD. Light was not the necessary factor for persisting circadian rhythm but influenced the period and amplitude of oscillation genes.

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Section: Introductionmentioning

confidence: 99%

Endogenous circadian time genes expressions in the liver of mice under constant darkness

Zhang

et al. 2020

BMC Genomics

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“…Tuco-tucos display tight nocturnal synchrony between activity and Tb rhythms in the lab (Tachinardi et al, 2014; Yassumoto et al, 2019). This tight synchrony has been resistant to several experimental manipulations, such as under a constant light regimen, when both activity and Tb rhythms split into 2 components of different periods (splitting) (Flôres et al, 2021) or when both activity and Tb switch from nocturnal to diurnal in respirometry chambers (Tachinardi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Subterranean tuco-tucos ( Ctenomys famosus ) are among the rodent species that display the abovementioned switch in activity time. In the laboratory light/dark (LD) conditions, tuco-tucos show robust nocturnal activity measured by wheel-running and IR sensors (Valentinuzzi et al, 2009; Yassumoto et al, 2019) and a tight temporal correlation between activity and Tb (Tachinardi et al, 2014). On the other hand, visual observations of tuco-tucos on the surface and automated recordings of light exposure in semi-field conditions strongly indicated that these animals would be diurnal in the wild (Tomotani et al, 2012; Flôres et al, 2016).…”

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Temporal Dissociation Between Activity and Body Temperature Rhythms of a Subterranean Rodent (Ctenomys famosus) in Field Enclosures

et al. 2023

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Several wild rodents, such as the subterranean tuco-tucos ( Ctenomys famosus), switch their time of activity from diurnal to nocturnal when they are transferred from field to the laboratory. Nevertheless, in most studies, different methods to measure activity in each of these conditions were used, which raised the question of whether the detected change in activity timing could be an artifact. Because locomotor activity and body temperature (Tb) rhythms in rodents are tightly synchronized and because abdominal Tb loggers can provide continuous measurements across field and laboratory, we monitored Tb as a proxy of activity in tuco-tucos transferred from a semi-field enclosure to constant lab conditions. In the first stage of this study (“Tb-only group,” 2012-2016), we verified high incidence (55%, n = 20) of arrhythmicity, with no consistent diurnal Tb rhythms in tuco-tucos maintained under semi-field conditions. Because these results were discrepant from subsequent findings using miniature accelerometers (portable activity loggers), which showed diurnal activity patterns in natural conditions ( n = 10, “Activity-only group,” 2016-2017), we also investigated, in the present study, whether the tight association between activity and Tb would be sustained outside the lab. To verify this, we measured activity and Tb simultaneously across laboratory and semi-field deploying both accelerometers and Tb loggers to each animal. These measurements ( n = 11, “Tb + activity group,” 2019-2022) confirmed diurnality of locomotor activity and revealed an unexpected loosening of the temporal association between Tb and activity rhythms in the field enclosures, which is otherwise robustly tight in the laboratory.

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“…These subterranean animals might not necessarily respond to daily and seasonal LD cycles like their epigeous counterparts. Nevertheless, our previous laboratory studies have shown that, in the 24 h scale, tuco-tucos display robust nocturnal running-wheel activity rhythms under LD12:12 conditions, and stable free-running rhythms in constant darkness (Valentinuzzi et al, 2009;Tachinardi et al, 2014;Yassumoto et al, 2019). Despite being subterranean and experiencing irregular light cycles in nature (Flôres et al, 2016), their phase response curve is similar to epigeous species (Flôres et al, 2013).…”

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confidence: 98%

Daylength Shapes Entrainment Patterns to Artificial Photoperiods in a Subterranean Rodent

Improta¹,

Flôres²,

Oda³

et al. 2022

J Biol Rhythms

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Photoperiodism plays an important role in the synchronization of seasonal phenomena in various organisms. In mammals, photoperiod encoding is mediated by differential entrainment of the circadian system. The limits of daily light entrainment and photoperiodic time measurement can be verified in organisms that inhabit extreme photic environments, such as the subterranean. In this experimental study, we evaluated entrainment of circadian wheel-running rhythms in South American subterranean rodents, the Anillaco tuco-tucos ( Ctenomys aff. knighti), exposed to different artificial photoperiods, from extremely long to extremely short photophases (LD 21:3, LD 18:6, LD 15:9, LD 9:15, LD 6:18 and LD 3:21). Artificial photoperiods synchronized their activity/rest rhythms and clear differences occurred in (a) phase angles of entrainment relative to the LD cycle and (b) duration of the daily activity phase α. These photoperiod-dependent patterns of entrainment were similar to those reported for epigeous species. Release into constant darkness conditions revealed aftereffects of entrainment to different photoperiods, observed in α but not in the free-running period τ. We also verified if animals coming from summer and winter natural photoperiods entrained equally to the artificial photoperiods by evaluating their phase angle of entrainment, α and τ aftereffects. To this end, experimental animals were divided into “Matching” and “Mismatching” groups, based on whether the experimental photoperiod (short-day [L < 12 h] or long-day [L > 12 h]) matched or not the natural photoperiod to which they had been previously exposed. No significant differences were found in the phase angle of entrainment, α and τ aftereffects in each artificial photoperiod. Our results indicate that the circadian clocks of tuco-tucos are capable of photoperiodic time measurement despite their natural subterranean habits and that the final entrainment patterns achieved by the circadian clock do not depend on the photoperiodic history.

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Acute effects of light and darkness on the activity and temperature rhythms of a subterranean rodent, the Anillaco tuco-tuco

Cited by 5 publications

References 39 publications

Endogenous circadian time genes expressions in the liver of mice under constant darkness

Endogenous circadian time genes expressions in the liver of mice under constant darkness

Temporal Dissociation Between Activity and Body Temperature Rhythms of a Subterranean Rodent (Ctenomys famosus) in Field Enclosures

Daylength Shapes Entrainment Patterns to Artificial Photoperiods in a Subterranean Rodent

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