Acute effects of epigallocatechin gallate from green tea on oxidation and tissue incorporation of dietary lipids in mice fed a high-fat diet

Abstract

Objective: To examine in mice the acute effects of epigallocatechin gallate (EGCG), a green tea bioactive polyphenol on substrate metabolism with focus on the fate of dietary lipids. Methods: Male C57BL/6 mice were fed high-fat diets supplemented with EGCG extracted from green tea (TEAVIGO, DSM Nutritional Products Ltd, Basel, Switzerland) at different dosages up to 1% (w/w). Effects of EGCG on body composition (quantitative magnetic resonance), food intake and digestibility, oxidation and incorporation of exogenous lipids (stable isotope techniques:13 C-labeled palmitate and diet supplemented with corn oil as a natural source of 13 C-enriched lipids) as well as gene expression (quantitative real-time PCR) in liver and intestinal mucosa were investigated. Results: Short-term supplementation (4-7 days) of dietary EGCG increased energy excretion, while food and energy intake were not affected. Fecal energy loss was accompanied by increased fat and nitrogen excretion. EGCG decreased post-prandial triglyceride and glycogen content in liver, increased oxidation of dietary lipids and decreased incorporation of dietary 13C-enriched lipids into fat tissues, liver and skeletal muscle. EGCG dose dependently reversed high-fat diet-induced effects on intestinal substrate transporters (CD36, FATP4 and SGLT1) and downregulated lipogenesis-related genes (ACC, FAS and SCD1) in liver in the post-prandial state. Conclusions: Anti-obesity effects of EGCG can be explained by a decreased food digestibility affecting substrate metabolism of intestinal mucosa and liver, leading to increased post-prandial fat oxidation and reduced incorporation of dietary lipids into tissues.

Identification of Mest/Peg1 gene expression as a predictive biomarker of adipose tissue expansion sensitive to dietary anti-obesity interventions

“…Dietary supplementation of EGCG, the green tea bioactive polyphenol, prevented the development of HFD-induced obesity in a dose-dependent manner in mice after 4 weeks (Klaus et al 2005). This was shown to be due to decreased intestinal fat absorption and transport (i.e., higher energy excretion) and increased oxidation of dietary lipids linked to a reduced incorporation of dietary lipids into various tissues (Friedrich et al 2012). Another intervention was the A B C D Fig.…”

“…The current investigation was undertaken with the primary aim to validate this idea. To this end, we conducted short-term (5 day) HFD challenge and intervention trials in mice applying, on top of the HFD, three different dietary interventions which have previously been shown to reduce the development of obesity and associated disorders albeit acting through different physiological mechanisms, i.e., supplementation of epigallocatechin gallate (EGCG) from green tea (Friedrich et al 2012), replacing part of the dietary lipids by n-3 PUFA (Kus et al 2011), and a high dietary protein content (Freudenberg et al 2012). Using quantitative real-time PCR gene expression in epididymal WAT, we identified gene expression of Leptin and Mest [mesoderm-specific transcript (Mest), also known as paternally expressed gene 1 (Peg1)] as early markers of adipose tissue expansion whose HFD-induced expression changes in fat tissue were normalized by the different anti-obesity interventions.…”

The Effect of Dietary Supplementation of Green Tea Catechins on Cardiovascular Disease Risk Markers in Humans: A Systematic Review of Clinical Trials

“…This is not the only potential mechanism, since others have also suggested the cholesterol-lowering effects of GT, including reducing capacity of hepatic cholesterol concentration and upregulation of hepatic LDL receptors [49][50][51]. The hypolipidemic effect of GTCs consumption is attributed to reduced intestinal absorption and decreased digestibility of cholesterol and dietary fats [52][53][54]. Additionally, EGCG has been identified to possess the potential to inhibit pancreatic lipase, contributing to the development of natural lipase inhibitors to prevent human obesity and dyslipidemia [55].…”

Exploring the possible applications of catechin (gel) for oral care of the elderly and disabled individuals

“…The effects of EGCG on antioxidant, dietary, obese, human malaria, cardiac muscle function, prostate cancer, innate immune receptor and cardiac muscle function has all been reported [50][51][52][53][54][55][56]. EGCG, exerts a growth inhibitory effect in cancer cells suggesting it possesses a promising anticancer potential due to its antioxidant, antimutagenic and chemopreventive properties [57].…”