Acute effects of capacitive and resistive electric transfer (CRet) on the Achilles tendon

Bito, Tsubasa; Tashiro, Yuto; Suzuki, Yoshishige; Kajiwara, Yuu; Zeidan, Hala; Kawagoe, Mirei; Sonoda, Takuya; Nakayama, Yasuaki; Yokota, Yuki; Shimoura, Kanako; Tatsumi, Makiko; Nakai, Kengo; Nishida, Yuichi; Yoshimi, Soyoka; Tsuboyama, Tadao; Aoyama, Tomoki

doi:10.1080/15368378.2019.1567525

Cited by 25 publications

(26 citation statements)

References 20 publications

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“…Also within the RF spectrum, non-invasive capacitive-resistive electric transfer (CRET) therapies apply 448-600 kHz electric currents in order to induce hyperthermia in target tissues, the thermal increase being a function of various physical and physiological parameters, including the specific impedance of the exposed tissue (Kotnik and Miklavcic 2000;Grimnes and Martinsen 2000). CRET therapies have been used successfully in regeneration of muscle (Takahashi et al 1999), bones (Kumaran and Watson 2019), tendons and ligaments (Bito et al 2019) and skin (Naranjo et al 2020). The block of in vitro experimental data has provided evidence that the regenerative effects of these therapies could be mediated by stimulation of stem cell proliferation through activation of proteins involved in cell cycle regulation (Hernandez-Bule et al 2014a) or by promotion of early chondrocyte differentiation through increased synthesis of cartilage-specific extracellular matrix molecules, such as collagen and glycosaminoglycans (Hernandez-Bule et al 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro stimulation with radiofrequency currents promotes proliferation and migration in human keratinocytes and fibroblasts

Hernández-Bule

Toledano-Macías

Naranjo

et al. 2021

Electromagnetic Biology and Medicine

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Capacitive-resistive electric transfer (CRET) therapies have been proposed as strategies for regeneration of cutaneous tissue lesions. Previous studies by our group have shown that intermittent stimulation with 448 kHz CRET currents at subthermal densities promotes in vitro proliferation of human stem cells involved in tissue regeneration. The present study investigates the effects of the in vitro exposure to these radiofrequency (RF) currents on the proliferation and migration of keratinocytes and fibroblasts, the main cell types involved in skin regeneration. The effects of the electric stimulation on cell proliferation and migration were studied through XTT and wound closure assays, respectively. The CRET effects on the expression and location of proteins involved in proliferation and migration were assessed by immunoblot and immunofluorescence. The obtained results reveal that electrostimulation promotes proliferation and/or migration in keratinocytes and fibroblasts. These effects would be mediated by changes observed in the expression and location of intercellular adhesion proteins such as β-catenin and E-cadherin, of proteins involved in cell-to-substrate adhesion such as vinculin, p-FAK and the metalloproteinase MMP-9, and of other proteins that control both processes: MAP kinases p-p38, p-JUNK and p-ERK1/2. These responses could represent a mechanism underlying the promotion of normotrophic wound regeneration induced by CRET. Indeed, electric stimulation would favor completion of granulation tissue formation prior to the closure of the outer tissue layers, thus preventing abnormal wound cicatrization or chronification.

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Section: Introductionmentioning

confidence: 99%

In vitro stimulation with radiofrequency currents promotes proliferation and migration in human keratinocytes and fibroblasts

Hernández-Bule

Toledano-Macías

Naranjo

et al. 2021

Electromagnetic Biology and Medicine

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“…1 Its positive effects include greater blood circulation, which allows the removal of inflammatory catabolites. 2,3 Bito et al 4 reported that CARE substantially improves blood circulation in the peritendinous region and increases haemoglobin saturation. In addition, CARE therapy warms deep tissue; 5 the resulting greater vascularization promotes the relaxation of tissues (especially muscular tissue) and drainage of swelling (oedema and haematoma).…”

Section: Introductionmentioning

confidence: 99%

Effects of capacitive and resistive electric transfer therapy in patients with painful shoulder impingement syndrome: a comparative study

et al. 2019

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Objective Capacitive and resistive electric transfer therapy (CARE) reduces pain and improves quality of life for many orthopaedic degenerative and inflammatory disorders. The research aim was to determine the effects of CARE on painful shoulder. The outcomes were pain reduction and recovery of shoulder function. Methods A retrospective, observational case-control study was conducted. Participants were 46 patients (22 in the CARE group and 24 in the SHAM group). Clinical data, pain (visual analogic scale, VAS) and functional scale scores (Disabilities of the Arm, Shoulder and Hand scale, and Constant–Murley Scale) were measured at baseline T0 (before treatment), T1 (after treatment) and follow-up T2 (2 months after the end of the treatment). Results VAS scores in the CARE group improved from 7.23 ± 1.11 at baseline to 2.68 ± 0.99 at follow-up. The SHAM group did not experience any improvement. Similarly, functional scale scores improved in the CARE group compared with the SHAM group. Conclusion Considering the small number of sessions needed, low cost and long-term benefits, CARE could be a useful therapeutic option for the conservative management of shoulder pain to restore pain-free and powerful movement to the shoulder joint.

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“…Due to Joule’s effect the RF current generates a thermal increase in the tissues that is a function of a number of physical and physiological parameters, including the specific impedance of each tissue [1, 2]. Hyperthermia induced by RF and microwave signals, either modulated or not, has been successfully applied in physiotherapeutic treatments for pain relief [3] or recovery of muscle, tendon and joint tissues [4–6], as well as in oncological treatments [7–10]. In the case of RF currents used in CRET therapies, in vitro experimental evidence exists providing some evidence on their potential applicability in oncology.…”

Section: Introductionmentioning

confidence: 99%

Response of neuroblastoma cells to RF currents as a function of the signal frequency

et al. 2019

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Background Capacitive-resistive electric transfer (CRET) is a non-invasive therapeutic strategy that applies radiofrequency electric currents within the 400–600 kHz range to tissue repair and regeneration. Previous studies by our group have shown that 48 h of intermittent exposure to a 570 kHz CRET signal at a subthermal density of 50 μA/mm 2 causes significant changes in the expression and activation of cell cycle control proteins, leading to cycle arrest in human cancer cell cultures. The present study investigates the relevance of the signal frequency in the response of the human neuroblastoma cell line NB69 to subthermal electric treatment with four different signal frequency currents within the 350–650 kHz range. Methods Trypan blue assay, flow cytometry, immunofluorescence and immunoblot were used to study the effects of subthermal CRET currents on cell viability, cell cycle progression and the expression of several marker proteins involved in NB69 cell death and proliferation. Results The results reveal that among the frequencies tested, only a 448 kHz signal elicited both proapoptotic and antiproliferative, statistically significant responses. The apoptotic effect would be due, at least in part, to significant changes induced by the 448 kHz signal in the expression of p53, Bax and caspase-3. The cytostatic response was preceded by alterations in the kinetics of the cell cycle and in the expression of proteins p-ERK1/2, cyclin D1 and p27, which is consistent with a potential involvement of the EGF receptor in electrically induced changes in the ERK1/2 pathway. This receives additional support from results indicating that the proapototic and antiproliferative responses to CRET can be transiently blocked when the electric stimulus is applied in the presence of PD98059, a chemical inhibitor of the ERK1/2 pathway. Conclusion The understanding of the mechanisms underlying the ability of slowing down cancer cell growth through electrically-induced changes in the expression of proteins involved in the control of cell proliferation and apoptosis might afford new insights in the field of oncology.

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Acute effects of capacitive and resistive electric transfer (CRet) on the Achilles tendon

Cited by 25 publications

References 20 publications

In vitro stimulation with radiofrequency currents promotes proliferation and migration in human keratinocytes and fibroblasts

In vitro stimulation with radiofrequency currents promotes proliferation and migration in human keratinocytes and fibroblasts

Effects of capacitive and resistive electric transfer therapy in patients with painful shoulder impingement syndrome: a comparative study

Response of neuroblastoma cells to RF currents as a function of the signal frequency

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