Acute Effects of Beclomethasone on Hyperpnea-Induced Bronchoconstriction

Abstract

We conclude that a single dose of BDP has an acute protective effect on the bronchial response to hyperpnea in both untrained subjects with asthma and athletes with EIB. This effect was associated with decreased excretion of urinary mediators, suggesting that BDP blunted the mast cell activation.

Urinary excretion of lipid mediators in response to repeated eucapnic voluntary hyperpnea in asthmatic subjects

“…In contrast, the levels of metabolites of thromboxane and isoprostanes remained unchanged, indicating specificity in the excretion of eicosanoids. Using the mass spectrometry platform, we also documented increased levels of CysLTs, and metabolites of PGD 2 following the EVH challenge, thus replicating and validating by mass spectrometry our laboratory's previous findings using EIA only (18,19). The increased urinary excretion of those two bronchoconstrictive mediators following both of the two repeated EVH challenges is similar to our previous findings of mediator excretion following repeated mannitol inhalation challenge (22).…”

“…The bronchoconstricting mediators released into the airways are rapidly removed by the circulation and are excreted into the urine (20, 27). Using enzyme immunoassay (EIA) methodology, our laboratory has previously demonstrated increased urinary excretion of cysteinyl leukotrienes (CysLTs) and the prostaglandin (PG) D 2 metabolites following exercise (28), EVH (18,19), and mannitol (22), respectively. Whereas CysLTs may be biosynthesized in many inflammatory cells, PGD 2 is almost exclusively produced by the mast cells, and its release, therefore, provides objective evidence of mast cell activation.…”

The Effect of Omega-3 Fatty Acids on Bronchial Hyperresponsiveness, Sputum Eosinophilia, and Mast Cell Mediators in Asthma

“…Th e eff ects of the dietary intervention on eicosanoid levels of 11 b -PGF 2 a and LTE 4 in urine were assessed by enzyme immunoassay, 11,14 and an ultraperformance liquid chromatographytandem mass spectrometry platform 16 that measures . 90 diff erent lipid mediators.…”

“…12 Evidence for mediator release to mannitol is an increase in urinary eicosanoids 11 b -PGF 2 a and LTE 4 following a mannitol challenge 11 similar to what has been observed with exercise and EVH. 13,14 BHR to mannitol identifi es EIB in elite athletes 15 and predicts the severity of EIB in people with asthma. 7 Considering these similarities, we evaluated whether dietary supplementation with omega-3 fatty acids could inhibit BHR to inhaled mannitol to assess the effi cacy of omega-3s in people with mild to moderate BHR.…”

Diagnostic Exercise Challenge Testing

“…The stimuli for indirect challenges to BHR encompass exercise, hypertonic saline, adenosine monophosphate (AMP), dry powder mannitol, and hyperpnea of cold or dry air or EVH ( [32]; to confirm asthma [30]; to evaluate new modalities for measuring airway narrowing [33]; and to evaluate two different bronchial provocation tests on day 1 [34] and define efficacy of medications [35][36][37]. This enhanced utilization of indirect tests is related to increased commercial availability of a standardized test kit for mannitol inhalation and also the increased need to define or identify EIB.…”