Acute Effect of Moringa Oleifera Raw Seeds on Blood Glucose, Serum Lipid and Liver Enzyme Profiles in Alloxan-Induced Diabetic Albino Wister Rats

Abstract

Background and Objective: Moringa oleifera has multiple uses and almost all parts of the plant form part of the traditional diet in many Tropical and Subtropical countries. It is not clear how the dried raw seed was eaten affects diabetes. Research has shown that the raw seed has a wide range of medical properties and nutritive values. Most of the plants used for diabetes mellitus are not edible therefore studying a plant with hypoglycemic activity would have to amerce value in the management of the disease. Material and Method: The diabetic state was confirmed after 72 hours of induction of diabetes with 150mg/kg of alloxan. The animals were separated into 5 groups (n=5). Group1 and 2 served as normal control (NC) and Diabetic non Treated (DNT) feed with normal chow and water ad libitum. Group 3 received metformin hydrochloride orally. Group 4 and 5 received 450mg and 350mg of raw Moringa oleifera powder orally. A weekly analysis of blood glucose from the tail vein was done. Animals were sacrificed at the end of 14 days and serum was collected for determination of serum lipids and enzymes profile. Result: Total cholesterol (TC), Triglyceride (TG), Low-density lipoprotein (LDL), Very low-density lipoprotein (VLDL) were seen to be reduced while High-density lipoprotein (HDL) was seen to be significantly increased at the end of two weeks. ALT, AST and ALP in the treatment groups at the end of the period were seen to be decreased significantly. Conclusion: Moringa oleifera Raw seeds given at doses 350mg, and 450mg/day showed anti hyperglycemic and hepatoprotective activity against alloxan induced diabetes in the rat.