Acute effect of cola and caffeine on locomotor activity in drosophila and rat

Kmeťová, Katarína; Marônek, Martin; Borbélyová, Veronika; Hodosy, Július; Celec, Peter

doi:10.33549/physiolres.934629

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Open arenas are regularly used in insect behavioral research, for example for the fruit fly Drosophila melanogaster (Kmetova et al, 2021) and in field crickets Gryllus campestris (Santostefano et al, 2016). Most studies quantify locomotory activity while thigmotaxis is not always analysed.…”

Section: Introductionmentioning

confidence: 99%

Open field behavior in the house cricket (Acheta domesticus): effect of illumination, sex differences and individual consistency

Vossen

Nilsson

Jansson

et al. 2023

Journal of Insects as Food and Feed

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Edible insects are worldwide promoted as an alternative protein, trace mineral and lipid source in animal feed and human food. The house cricket (Acheta domesticus) is already being reared at an industrial scale, yet current mass-rearing practices and facility design may still leave room for improvement. Behavioral tests have been suggested as an important assessment tool at the whole-organism level that can be used to find optimal housing conditions (e.g. density, diet, temperature). Here, we adapt the widely used open field test to the house cricket. We tested 16 male and 16 female house crickets four times under two different light intensities. Videos were analysed with Ethovision™ tracking software and variables distance moved, velocity, and duration and frequency in zone were extracted. Results showed that house crickets, like vertebrate model species, spent most time close to the walls of the arena, and crossed the center zone with high velocity. Brighter illumination was associated with increased velocity, in particular in the center zone during the first test occasion, suggesting avoidance of this zone. Male crickets had higher locomotory activity than females. Consistency repeatabilities and intraclass correlation coefficients (ICCs) were moderate to high, and the correlation between subsequent occasions became stronger over the four occasions at day 1, 2, 3 and 7. The first test occasion differed from subsequent occasions, therefore repeated testing may be necessary when analysing experimental manipulations of small effect size. Overall, the results are promising for use of the open field test as a precise phenotyping tool.

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Section: Introductionmentioning

confidence: 99%

Open field behavior in the house cricket (Acheta domesticus): effect of illumination, sex differences and individual consistency

Vossen

Nilsson

Jansson

et al. 2023

Journal of Insects as Food and Feed

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Monitoring of Caffeine Consumption Effect on Skin Blood Properties by Diffuse Reflectance Spectroscopy

Milanic,

Hren,

Stergar

et al. 2024

Physiol Res

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Caffeine is the most widely consumed psychoactive substance worldwide, affecting numerous tissues and organs, with notable impacts on the central nervous system, heart, and blood vessels. The effect of caffeine on vascular smooth muscle cells is an initial transient contraction followed by significant vasodilatation. In this study we investigate the use of diffuse reflectance spectroscopy (DRS) for monitoring of vascular changes in human skin induced by caffeine consumption. DRS spectra were recorded on volar sides of the forearms of eight healthy volunteers at time intervals of 0, 30, 60, 120, and 180 min after consumption of caffeine, while one subject served as a negative control. Analytical diffusion approximation solutions for diffuse reflectance from three-layer structures were used to assess skin composition (e.g. dermal blood volume fraction and oxygen saturation) by fitting these solutions to experimental data. The results demonstrate that cutaneous vasodynamics induced by caffeine consumption can be monitored by DRS, while changes in the control subject not consuming caffeine were insignificant.

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Acute effect of cola and caffeine on locomotor activity in drosophila and rat

Cited by 2 publications

References 26 publications

Open field behavior in the house cricket (Acheta domesticus): effect of illumination, sex differences and individual consistency

Open field behavior in the house cricket (Acheta domesticus): effect of illumination, sex differences and individual consistency

Monitoring of Caffeine Consumption Effect on Skin Blood Properties by Diffuse Reflectance Spectroscopy

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