Acute ecotoxicity bioassay using Dendrocephalus brasiliensis: alternative test species for monitoring of contaminants in tropical and subtropical freshwaters

Santos, Vanessa Santana Vieira; Campos, Carlos Fernando; Júnior, Edimar Olegário de Campos; Pereira, Boscolli Barbosa

doi:10.1007/s10646-018-1951-3

Cited by 13 publications

(7 citation statements)

References 24 publications

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“…Considering the presented scenario, the choice of native species for conservational efforts or productive activities must prevail when possible. Some species present characteristics that made its use viable in several areas, as such Dendrocephalus brasiliensis, which can be used in conservational efforts as a test organism in toxicity [4], in the residuary water treatment [5], or in productive activity of aquaculture [6]. The utilization of native species, such as, Dendrocephalus brasiliensis, avoids the invasive ones that can cause alterations in the environment; but for the successful adoption of a species, independent of its use, the development of technologies aiming to facilitate its management is necessary.…”

Section: Introductionmentioning

confidence: 99%

Recognizing and counting Dendrocephalus brasiliensis (Crustacea: Anostraca) cysts using deep learning

Astolfi

Ferreira

et al. 2021

PLoS ONE

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The Dendrocephalus brasiliensis, a native species from South America, is a freshwater crustacean well explored in conservational and productive activities. Its main characteristics are its rusticity and resistance cysts production, in which the hatching requires a period of dehydration. Independent of the species utilization nature, it is essential to manipulate its cysts, such as the counting using microscopes. Manually counting is a difficult task, prone to errors, and that also very time-consuming. In this paper, we propose an automatized approach for the detection and counting of Dendrocephalus brasiliensis cysts from images captured by a digital microscope. For this purpose, we built the DBrasiliensis dataset, a repository with 246 images containing 5141 cysts of Dendrocephalus brasiliensis. Then, we trained two state-of-the-art object detection methods, YOLOv3 (You Only Look Once) and Faster R-CNN (Region-based Convolutional Neural Networks), on DBrasiliensis dataset in order to compare them under both cyst detection and counting tasks. Experiments showed evidence that YOLOv3 is superior to Faster R-CNN, achieving an accuracy rate of 83,74%, R2 of 0.88, RMSE (Root Mean Square Error) of 3.49, and MAE (Mean Absolute Error) of 2.24 on cyst detection and counting. Moreover, we showed that is possible to infer the number of cysts of a substrate, with known weight, by performing the automated counting of some of its samples. In conclusion, the proposed approach using YOLOv3 is adequate to detect and count Dendrocephalus brasiliensis cysts. The DBrasiliensis dataset can be accessed at: https://doi.org/10.6084/m9.figshare.13073240.

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Section: Introductionmentioning

confidence: 99%

Recognizing and counting Dendrocephalus brasiliensis (Crustacea: Anostraca) cysts using deep learning

Astolfi

Ferreira

et al. 2021

PLoS ONE

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“…Enquanto as análises químicas identificam e quantificam as concentrações das substâncias tóxicas, os testes de toxicidade avaliam o efeito dessas substâncias sobre sistemas biológicos. Sendo assim, as análises químicas e os testes ecotoxicológicos se complementam (COSTA et al, 2008a;SANTOS et al, 2018;WALKER et al, 2012).…”

Section: Testes Ecotoxicológicosunclassified

“…Essas substâncias também são utilizadas para definir as condições essenciais dos ensaios para cada organismo-teste, podendo ser espécies aquáticas ou terrestres. Uma vez estabelecidas essas condições ótimas de ensaio, os resultados tornam-se comparáveis, ou seja, apresentam "boa" repetibilidade e reprodutibilidade (MAGALHÃES; FERRÃO-FILHO, 2008;NEWMAN, 2010;SANTOS et al, 2018;BERTOLETTI, 2006). WESTERMAN, 1985;NEWMAN, 2010;RAND, 1995;SCHVARTSMAN, 1991;VAN LEEUWEN, 2007).…”

Section: Testes Ecotoxicológicosunclassified

Bioensaios de toxicidade utilizando invertebrados aquáticos em exposição ao Alquilbenzeno Linear Sulfonado

Felipe¹

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Ao Pai Celestial, o Grande Arquiteto do Universo, sempre presente, por todas as oportunidades que me proporcionara, e por sempre ter me oferecido saúde, disposição e força para vencer obstáculos. Aos meus pais, Gilberto Felipe e Vera Júlia Brugnoli Felipe, por todo amor dispensado, por todo apoio e gesto de carinho durante meus anos de doutorado. Obrigado por me ensinarem tudo que sei em minha existência e obrigado por nunca terem desistido de mim. Ao meu irmão Danilo Rafael Felipe, à minha cunhada Flávia Gásparo Felipe e ao meu sobrinho Felipe Gásparo Felipe, por todo gesto de carinho proporcionado, aos almoços de domingo reunidos na casa do pai, isso sempre fez toda a diferença na minha vida. À minha família de São Carlos, Sanca Badalada, por terem dividido comigo quatro anos de amor, partilha, companheirismo e festinhas, afinal não é só de trabalho que se vive uma doutoranda. Ao meu orientador Professor Juliano José Corbi, possuidor de inigualável saber, pela oportunidade, presteza, atenção e orientação durante meu período de doutorado. Obrigado por cada "puxão de orelha" que me impulsionaram a dar o máximo de mim. Obrigada também pelo companheirismo nesses anos de trabalho, foi muito bom trabalhar com você. À professora Maria Bernadete Varesche pela possibilidade de trabalho com os reatores e por todo ensinamento. Aos professores Guilherme Gorni e Maria Bernadete Varesche, pelas sugestões e contribuições na banca de qualificação.

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“…On the other hand, there is a growing need to evaluate the realistic impacts on autochthonous species in tropical freshwaters, as most ecotoxicological studies is performed with acclimated exotic species from temperate areas (Freitas and Rocha 2011;Rodgher and Espíndola 2008;Artal et al 2017). Few studies in tropical and subtropical areas have been conducted with local species, and the use of temperate organisms constitutes a limiting factor for aquatic toxicity assessment of pollutants (Santos et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Also, studies have been encouraged owing the short life cycle, abundance in environment, thermal tolerance and high reproduction rate of the species. Owing these properties, D. brasiliensis are emerging as an attractive alternative test organism for monitoring of contaminants in tropical and subtropical freshwaters(Santos et al 2018).To further assess the environmental impact of synthetic and biogenic surfactants, this study aimed to evaluate the ecotoxicity of the common industrial surfactants, sodium dodecyl sulfate (SDS) and triton X-100, and three biosurfactants, surfactin, iturin and fengycin, at different concentrations on the microcrustaceans D. magna and D. brasiliensis, in order to assess ecotoxicological parameters through acute toxicity tests and to compare the sensitivity of tropical and temperate species.…”

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confidence: 99%

Avaliação ecotoxicológica de tensoativos de origem química e biológica

Santos¹

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The employment on a global scale and the increasing release of surfactants on the ecosystems cause the accumulation of their residues on environment, consequently altering its original characteristics and whose potential impacts on organisms remain not elucidated. Ecotoxicology is an essential science regarding the assessment and knowledge about the impact of toxic compounds on biological systems. The waterflea Daphnia magna is one of the most sensitive species of ecotoxicological standardized assays. However, it is also important to consider the use of autochthonous species, such as Dendrocephalus brasiliensis, in studies performed in tropical regions, since they attend the specificities of local ecosystems and provide realistic results. In this sense, the aim of this study was to evaluate the ecotoxicological parameters for chemical surfactantssodium dodecyl sulphate and Triton X-100and biogenic surfactantssurfactin, iturin and fengycinat different concentrations using D. magna as model organism and D. brasiliensis as the representative species of tropical freshwaters. Data obtained through acute toxicity tests revealed a dose-dependent for all chemicals. D. brasiliensis showed lower EC50-48h values than D. magna considering all the tested compounds, indicating a higher sensitivity of the tropical species in relation to the species from temperate regions. The results confirmed an environmentally safe behaviour of fengycin and iturin. Nevertheless, surfactin was the less safety biosurfactant for both organisms, indicating that even biological compounds are able to cause deleterious effects on living organisms. Also, the synthetic surfactants exhibited high toxicity effects, as the compounds showed immobility impacts on both species even in low concentrations. Evaluations considering the recommended dosage revealed a higher toxicity of SDS for both species. The higher responsiveness of D. brasiliensis highlights the organism as a promising tool for ecotoxicological studies regarding tropical climates.

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Acute ecotoxicity bioassay using Dendrocephalus brasiliensis: alternative test species for monitoring of contaminants in tropical and subtropical freshwaters

Cited by 13 publications

References 24 publications

Recognizing and counting Dendrocephalus brasiliensis (Crustacea: Anostraca) cysts using deep learning

Recognizing and counting Dendrocephalus brasiliensis (Crustacea: Anostraca) cysts using deep learning

Bioensaios de toxicidade utilizando invertebrados aquáticos em exposição ao Alquilbenzeno Linear Sulfonado

Avaliação ecotoxicológica de tensoativos de origem química e biológica

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