Acute Cholecystitis Reduces Interstitial Cells of Cajal in Porcine Gallbladder Through Decreased mRNA Synthesis

Huang, Zhenpeng; Qiu, Hu; Yu, Baoping

doi:10.1159/000489987

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…The significant downregulation of SCF/c‐kit pathway is associated with a decrease in the number of gallbladder ICCs, increased apoptosis of gallbladder ICCs, and a decrease in the maintenance and regeneration of ICC networks (Hu, Luo, & Ding, 2009; Torihashi et al, 1999). Our previous study supports this, as inhibiting inflammation leads to the upregulation of the SCF/c‐kit pathway (Huang, Qiu, & Yu, 2018). Here we further found that the removal of stress allows kit receptors to recover, as the SCF/c‐kit pathway was upregulated and the number of gallbladder ICCs increased.…”

Section: Discussionsupporting

confidence: 75%

The impact of acute stress disorder on gallbladder interstitial cells of Cajal

Huang

Qiu

Wang

et al. 2020

Journal Cellular Physiology

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Physical and psychological stress exerts a substantial effect on gastrointestinal motility disorders, where trauma enhances symptoms of digestive dysfunction. Interstitial cells of Cajal (ICCs) act as pacemakers for gastrointestinal motility regulation and are likely important in stress‐associated gastrointestinal motility disorders. This study explored the mechanisms underlying gallbladder ICCs function under acute stress conditions using a rabbit chest puncture and cholecystectomy model. The stem cell factor (SCF)/c‐kit pathway is essential for the development of ICCs, and gene expression was investigated to identify stress‐induced transcriptional alterations. Immunohistochemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling assays were used to determine ICCs apoptosis, whereas western blot analysis and reverse‐transcription polymerase chain reaction were used to detect changes in the SCF/c‐kit signaling pathway. These methods revealed a reduction in ICCs via apoptosis following stress, and ICCs increased over time after stressor removal. Therefore, this study demonstrates the impact of stress on ICCs development and survival and further confirms the link between stress and gastrointestinal motility.

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Section: Discussionsupporting

confidence: 75%

The impact of acute stress disorder on gallbladder interstitial cells of Cajal

Huang

Qiu

Wang

et al. 2020

Journal Cellular Physiology

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“…Hemorrhage, edema, surface ulceration, and fibroblast proliferation were assigned scores of 0, 1, 2, and 3, representing no, mild, moderate, and severe conditions, respectively. The presence or absence of vascular dilation was noted and given a score of 1 if present and 0 if absent . Scores were summed and the final inflammation score ranged from 0 to 7.…”

Section: Methodsmentioning

confidence: 99%

“…The presence or absence of vascular dilation was noted and given a score of 1 if present and 0 if absent. 10 Scores were summed and the final inflammation score ranged from 0 to 7. The inflammation scores were determined by a pathologist in a blinded fashion at the Department of Pathology of the First Affiliated Hospital of Zhengzhou University.…”

Section: Histological Analysismentioning

confidence: 99%

Changes in bladder function with time following cystostomy in rats

Chen

Wen

et al. 2019

Neurourology and Urodynamics

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Aims: To investigate bladder function patterns following cystostomy and determine the best time window for cystometric evaluation of bladder function in conscious rats.Materials and Methods: Cystostomy was performed in rats of the first seven groups; thereafter, cystometry was performed in the designed time interval.Noncystostomy rats of group 8 voided freely as control. Basal bladder pressure (P ves.basal ), maximum bladder pressure (P ves.max ), bladder threshold pressure (P ves.thre ), voiding interval (VI), bladder contraction duration (CD) , bladder compliance (ΔC), voided volume (VV), postvoiding residual urine (PVR), and bladder capacity (BC) were recorded and compared with cystostomy groups, with VV, PVR, BC compared with the control values. Bladders were collected after the urodynamic study for weighing, hematoxylin-eosin, and Masson staining to investigate pathological changes.Results: P ves.basal , P ves.max , and P ves.thre trended downward, while BC, VI, VV, and ΔC trended upward on days 1 to 5 postcystostomy. BC and VV significantly decreased on days 1 to 3 postcystostomy compared with control values; on days 5 to 15 postcystostomy, P ves.basal , P ves.max , P ves.thre , VI, VV, BC, and PVR were stable, and BC, VV, and PVR showed no significant differences from the control values. However, on day 21 postcystostomy, BC increased significantly compared with the controls. Bladder weight increased in the cystostomy groups compared with the controls. Pathological analysis showed severe acute bladder inflammation on days 1 to 3, mild inflammation on days 5 to 15, and increased collagen deposition in bladder tissue on day 21 postcystostomy. Conclusion: Cystometric evaluation of bladder function in conscious rats is best performed on days 5 to 15 postcystostomy. K E Y W O R D Sbladder, collagen, cystometry, cystostomy, HE staining, Masson staining, rat

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“…ICC is generally considered as a pacemaker cell and a neuromuscular transmission medium of gastrointestinal activity. Studies have shown that ICC density in colon of patients with slow transit constipation is significantly lower than that of normal patients (Huang et al, 2018). Therefore, the decrease of ICC may lead to the lack of slow wave activity, which affects the contraction response and leads to delayed transmission in patients with slow transit constipation.…”

Section: Introductionmentioning

confidence: 98%

Effect of miR-129-3p on autophagy of interstitial cells of Cajal in slow transit constipation through SCF C-kit signaling pathway

et al. 2022

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Objective: To explore the mechanism by which miR-129-3p affected the autophagy of interstitial cells of Cajal (ICCs) in slow transit constipation tissues through the SCF C-kit signaling pathway. Methods: Colon samples from 20 Slow transit constipation (STC) patients who underwent total colectomy plus ileorectal anastomosis or subtotal colon resection plus anti-peristaltic rectal anastomosis were collected in our hospital. The colon of 20 non-STC patients was used as control. The control of this study was 20 patients undergoing radical surgery for colon cancer (left colon cancer) in our hospital. Fifty healthy SPF Kunming mice were purchased from Liaoning Changsheng Biotechnology Co., Ltd. Results: The mRNA expression of miR-129-3p in the STC group was lower than that in the control group (CTLR) group (P<0.05). The mRNA expression of miR-129-3p in STC group was lower than that in the NC group (P<0.05), and mRNA expression in STC+miR-129-3p group was higher than that in STC+miR-NC group (P<0.05). In the first week, the weight of dry and wet feces of the STC group was lower than that of the NC mice (P<0.05), and the weight of dry feces and wet feces of the STC group was lower than that of the NC group at the 2, 3, and 4 weeks, STC+miR-129 -3p was higher than that in the STC group (P<0.05). Conclusion: The increased expression of C-kit and SCF regulated by miR-129-3p contributed to the protection of interstitial cells. Knockdown of miR-129-3p expression could inhibit the activation of AKT/mTOR signaling pathway, reduce cell proliferation activity.

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Acute Cholecystitis Reduces Interstitial Cells of Cajal in Porcine Gallbladder Through Decreased mRNA Synthesis

Cited by 7 publications

References 32 publications

The impact of acute stress disorder on gallbladder interstitial cells of Cajal

The impact of acute stress disorder on gallbladder interstitial cells of Cajal

Changes in bladder function with time following cystostomy in rats

Effect of miR-129-3p on autophagy of interstitial cells of Cajal in slow transit constipation through SCF C-kit signaling pathway

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