Acute bacterial prostatitis: two different sub-categories according to a previous manipulation of the lower urinary tract

Millán-Rodríguez, F.; Palou, Joan; Bujons-Tur, Anna; Musquera-Felip, Mireia; Sevilla-Cecilia, Carlota; Serrallach-Orejas, Marc; Baez-Angles, Carlos; Villavicencio-Mavrich, H.

doi:10.1007/s00345-005-0040-4

Cited by 80 publications

(50 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First, the bacterial-infection model is not representative of all types of human prostatitis, because many patients with chronic prostatitis and chronic pelvic pain syndrome have no evidence of urinary tract infection (43). In addition, E. coli is not the only source for bacterial infection (39,44,45). Second, transurethral instillation of uropathogenic bacteria usually causes an immediate phase of acute inflammation followed a few months later by a second phase of multifocal chronic inflammation (34).…”

Section: Discussionmentioning

confidence: 99%

Prostatic inflammation enhances basal-to-luminal differentiation and accelerates initiation of prostate cancer with a basal cell origin

Kwon¹,

Zhang²,

Ittmann

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

168

154

View full text Add to dashboard Cite

Chronic inflammation has been shown to promote the initiation and progression of diverse malignancies by inducing genetic and epigenetic alterations. In this study, we investigate an alternative mechanism through which inflammation promotes the initiation of prostate cancer. Adult murine prostate epithelia are composed predominantly of basal and luminal cells. Previous studies revealed that the two lineages are largely self-sustained when residing in their native microenvironment. To interrogate whether tissue inflammation alters the differentiation program of basal cells, we conducted lineage tracing of basal cells using a K14-CreER; mTmG model in concert with a murine model of prostatitis induced by infection from the uropathogenic bacteria CP9. We show that acute prostatitis causes tissue damage and creates a tissue microenvironment that induces the differentiation of basal cells into luminal cells, an alteration that rarely occurs under normal physiological conditions. Previously we showed that a mouse model with prostate basal cell-specific deletion of Phosphatase and tensin homolog (K14-CreER;Pten fl/fl ) develops prostate cancer with a long latency, because disease initiation in this model requires and is limited by the differentiation of transformation-resistant basal cells into transformation-competent luminal cells. Here, we show that CP9-induced prostatitis significantly accelerates the initiation of prostatic intraepithelial neoplasia in this model. Our results demonstrate that inflammation results in a tissue microenvironment that alters the normal prostate epithelial cell differentiation program and that through this cellular process inflammation accelerates the initiation of prostate cancer with a basal cell origin.prostate stem cells | cells-of-origin for cancer

show abstract

Section: Discussionmentioning

confidence: 99%

Prostatic inflammation enhances basal-to-luminal differentiation and accelerates initiation of prostate cancer with a basal cell origin

Kwon¹,

Zhang²,

Ittmann

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

168

154

View full text Add to dashboard Cite

show abstract

“…Of all chronic prostatitis of different types, chronic nonbacterial prostatitis (IIIa) or chronic prostatitis/chronic pelvic pain syndrome (IIIb) is the most common and accounts for about 95% of chronic prostatitis (18,19). Generally, chronic prostatitis is characterized by refractory pelvic or perineal pain without evidence of urinary tract infection, which is usually accompanied by bladder and urethra dysfunction.…”

Section: Discussionmentioning

confidence: 99%

“…The quality of RNA was determined by methanal agarose gel electrophoresis following ethidium bromide staining. Total RNA (2µg) was subjected to reverse transcription using the Reverse Transcription System (Jikang, Shanghai, China) with random primer oligo(DT) 18 (0.5µg). The reaction conditions were as follows: 70º C for 5 min, 37º C for 60 min.…”

Section: Detection Of Heat Pain Thresholdmentioning

confidence: 99%

P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats

Zhang

Liu

et al. 2013

Int. braz j urol.

View full text Add to dashboard Cite

Purpose: Evidence shows that adenosine triphosphate (ATP) is involved in the transmission of multiple chronic pain via P 2 X 7 receptor. This study was to investigate the P 2 X 7 and microglial cells in the chronic prostatitis pain. Materials and Methods: Rats were divided into control group and chronic prostatitis group (n = 24 per group). A chronic prostatitis animal model was established by injecting complete Freund's adjuvant (CFA) to the prostate of rats, and the thermal withdrawal latency (TWL) was detected on days 0, 4, 12 and 24 (n = 6 at each time point in each group). Animals were sacrificed and the pathological examination of the prostate, detection of mRNA expression of P 2 X 7 and ionized calcium binding adaptor molecule 1 (IBA-1) and measurement of content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the dorsal horn of L 5 -S 2 spinal cord were performed on days 0, 4, 12 and 24. In addition, the content of TNF-α and IL-1β in the dorsal horn of L 5 -S 2 spinal cord was measured after intrathecal injection of inhibitors of microglial cells and/or P 2 X 7 for 5 days. Results: The chronic prostatitis was confirmed by pathological examination. The expression of P 2 X 7 and IBA-1 and the content of TNF-α and IL-1β in rats with chronic prostatitis were significantly higher than those in the control group. On day 4, the expressions of pro-inflammatory cytokines became to increase, reaching a maximal level on day 12 and started to reduce on day 24, but remained higher than that in the control group. Following suppression of microglial cells and P 2 X 7 receptor, the secretion of TNF-α and IL-1β was markedly reduced. Conclusion: In chronic prostatitis pain, the microglial cells and P 2 X 7 receptor are activated resulting in the increased expression of TNF-α and IL-1β in the L 5 -S 2 spinal cord, which might attribute to the maintenance and intensification of pain in chronic prostatitis. INTRODUCTIONChronic prostatitis, a common urological condition in young and middle-age men, is caused by multiple etiological factors. Pain is a major presentation of chronic prostatitis (1). Previous studies focused on the pathological changes in the prostate, while the pathways related to neurotransmission and the regulatory mechanisms of chronic prostatitis pain have not been studied. Recent studies have identified the chronic prostatitis pain as a visceral referred pain, which is usually accompanied by the dysfunction of pelvic floor muscles. The prostate is innervated largely by the pelvic nerves arising from the L 5 -S 2 spinal cord (2,3). P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats _______________________________________________ 277It has also been shown that the transmission and regulation of pain are associated with not only the neurons but the microglia and astrocytes (4,5). Studies also demonstrated that astrocytes and microglias may secrete pro-inflammatory cytokines such as tumor necrosis factors (TNF), interleukin-1 (IL-1), nerve growth fac...

show abstract

“…and Enterococcus spp. [35][36][37]. It is still under debate if and to what extent C. trachomatis can cause prostatitis.…”

Section: Prostatitismentioning

confidence: 99%

The Role of Chlamydia trachomatis in Male Infertility

et al. 2012

View full text Add to dashboard Cite

Acute bacterial prostatitis: two different sub-categories according to a previous manipulation of the lower urinary tract

Cited by 80 publications

References 11 publications

Prostatic inflammation enhances basal-to-luminal differentiation and accelerates initiation of prostate cancer with a basal cell origin

Prostatic inflammation enhances basal-to-luminal differentiation and accelerates initiation of prostate cancer with a basal cell origin

P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats

The Role of Chlamydia trachomatis in Male Infertility

Contact Info

Product

Resources

About