Acute and Subacute Toxicological Assessment of the Leaf Aqueous Extract of Eremomastax speciosa (Acanthaceae) in Wistar Rats

Siwe, Gaël; Enow-Orock, George; Amang, André Perfusion; Mezui, Christophe; Dongmo, Alain Bertrand; Tan, Paul V.

doi:10.9734/jamps/2015/18361

Cited by 10 publications

(9 citation statements)

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“…It is believed that medicinal plants in high dose could cause toxic or even lethal effects when administered in vivo (Silveira, Bandeira & Arrais, 2008;Siwe et al, 2015). Results from this study showed no difference in ROW in the treatment group when compared to control group, which indicates that LA is safer to the internal organs, supported by an earlier study published by Porwal, Khan & Maheshwari (2017).…”

Section: Discussionsupporting

confidence: 75%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

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Background Lauric acid (LA), a common constituent of coconut oil, is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods The study was conducted in accordance with the Organization for Economic Co-operation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2,000 mg/kg body weight, respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results The study results showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion The current finding suggests that single oral administration of LA, even up to 2,000 mg/kg body weight, did not exhibit any signs of toxicity in SD rats; thus, it was safe to be used on disease models in animals.

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Section: Discussionsupporting

confidence: 75%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

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“…Indeed, measurement of these proteins can reflect nutritional status and be used to screen or help diagnose kidney and liver diseases as well as many other conditions. High levels of total protein can be observed with chronic inflammation or liver infections [18]. In this study, the significant increase in serum concentration of total protein in the treated animals further suggests the alteration the synthetic functions of the liver.…”

Section: Hematologicalsupporting

confidence: 59%

“…Another important indicator of toxicity is the change in organ weight that is usually determined in subchronic and chronic toxicity tests [17]. Indeed, plant-derived products, when ingested, can be toxic to organs such as kidneys, liver, spleen, stomach and lungs due to their various function in the body [18]. The variations observed in liver, lung, kidneys and ovary were not gender nor dose responsive dependent; given that, macroscopic observation of these treated rat organs showed no abnormality in their morphology during the 28 days of experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the significant decrease in platelet count obtained in all AESI treated animals could be due to the fact that the extract inhibited the production of thrombopoietin. This decrease may also be attributed to the presence in the extract of metabolites which pharmacological effect are similar to diuretics, ibuprofen, aspirin and chloramphenicol [18,23].…”

Section: Hematologicalmentioning

confidence: 99%

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Evaluation of Acute and Sub-acute Toxicity of the Aqueous Extract from the Fruit of Solanum indicum Linn. (Solanaceae) in Rats

Epoh

Dongmo

Tadjoua

et al. 2019

EJMP

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Aim: The fruit of Solanum indicum Linn have been reported traditionally to have anti-hypertensive and diuretic properties. This study was undertaken to evaluate the toxicological potential of S. indicum fruits aqueous extract through the acute and sub-acute toxicity tests in rats. Methodology: For acute toxicity evaluation, a single oral dose of 5000 mg/kg of the plant extract was administrated in 60 days old female albino Wistar rats. Then, the animals were observed for 14 days. Sub-acute toxicity studies were conducted with 50 adult rats of both gender that orally received during 28 days, increasing doses of the plant extract. Their body weight and food intake were weekly collected. At the end of the experiment, biochemical and hematological parameters as well as histological analysis of organs (liver, kidneys and spleen) were undertaken. Results: Single oral administration of 5000 mg/kg dose of the fruit plant aqueous extract produced no mortality or signs of toxicity. During sub-acute test, no variations in body weight and food intake of both animals gender were observed. An important decrease in male’s rat liver weight were obtained at the dose 25 mg/kg; serum urea, total cholesterol, TAG, ALP and AST levels were signiﬁcantly lowered in male especially at the dose 50 mg/kg, but this decrease was noticed only in serum urea, ALP and ALT in female rats. Furthermore, a significant decrease in platelets number, serum PCT, MPV and PDW levels were recorded in all treated male rats except those receiving the highest extract dose. No structural changes in treated animal organs section histology were observed when compared to controls. Conclusion: The fruits aqueous extracts of S. indicum is safe when administered acutely and for 28 days in rats. However, alterations on their hematological and biochemical parameters were not closely related with the dose, implying caution on its use.

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“…Manuscript to be reviewed organ to body weight index, which is important and critical in determining drug toxicity linked to treatment-related effects (Sellers et al 2007). It is believed that medicinal plants in high dose could cause toxic or even lethal effects when administered in vivo (Silveira et al 2008;Siwe et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Sari¹

2020

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Background: Lauric acid (LA), a common constituent of coconut oil is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective: The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods: Study was conducted in accordance with the Organization for Economic Cooperation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2000 mg/kg body weight respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15 th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results: Study result showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion: Current finding suggests that single oral administration of LA, even up to 2000 mg/kg body weight did not exhibit any signs of toxicity in SD rats, thus, safe to be used on disease model in animals.

show abstract

Acute and Subacute Toxicological Assessment of the Leaf Aqueous Extract of Eremomastax speciosa (Acanthaceae) in Wistar Rats

Cited by 10 publications

References 0 publications

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Evaluation of Acute and Sub-acute Toxicity of the Aqueous Extract from the Fruit of Solanum indicum Linn. (Solanaceae) in Rats

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

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