Acute and subacute toxicity evaluation of ethanol extract from aerial parts of Epigynum auritum in mice

Yang, Meilian; Wu, Zhengtao; Wang, Yudan; Kai, Guoyin; Njateng, Guy Sedar Singor; Cai, Shengbao; Cao, Jun; Cheng, Guiguang

doi:10.1016/j.fct.2019.05.042

Cited by 57 publications

(34 citation statements)

References 33 publications

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“…Meanwhile, the activity of adrenocortical hormone may initiate negative feedback regulation, thereby inhibit the productions of red blood cells and hemoglobin [38]. Moreover, a significant increase of PCT in 1000 mg/kg group (both male and female) may be caused by the increase of PLT, which is consistent with the reported results [39]. Daily oral administration of MHTA at high concentration for 28 days may affect the glucocorticoid hyperactivity, and lead to liver damage.…”

Section: Aggregationssupporting

confidence: 89%

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Yang

Wang

Fan

et al. 2020

Nat. Prod. Bioprospect.

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Melodinus henryi is a good source of terpenoid indole alkaloids, and traditionally used as a folk medicine in the treatment of meningitis and fracture. In order to further exploit their potential uses, its anti-inflammatory and immunosuppressive activities, safety evaluations and chemical profiles have been illustrated. Compared to the crude methanol extract from M. henryi and its non-alkaloidal fraction, the total alkaloidal fraction (MHTA) had the strongest anti-inflammatory and immunosuppressive activities. In the acute oral toxicity assay, the half lethal dose (LD 50) of MHTA was more than 2000 mg/kg. The sub-acute toxicity assay for consecutive 28 days exhibited MHTA at a lower concentrations of less than 500 mg/kg might be regarded as safe, and might damage spleen, liver, kidney, and heart when the dose is higher than 1000 mg/kg. In addition, a phytochemical investigation on MHTA led to the isolation of 15 monoterpenoid indole alkaloids. Thus, in regard with the potent side effects of MHTA, it should be used with caution in the development of phytomedicine. Meilian Yang and Yudan Wang have contributed equally to this work.

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Section: Aggregationssupporting

confidence: 89%

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Yang

Wang

Fan

et al. 2020

Nat. Prod. Bioprospect.

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“…The use of natural products has developed more interest and confidence due to their safety and efficacy among people of all age groups (Amos et al, 2015). However, there is a lack of scientific data on the evidence based platform as well as toxicological investigations of these natural medicines (Yang et al, 2019). The toxicological information and safety profile of new compounds is of prime value, enabling us to choose an appropriate dosage in animal studies at preclinical level.…”

Section: Introductionmentioning

confidence: 99%

Oral toxicity of arjunolic acid on hematological, biochemical and histopathological investigations in female Sprague Dawley rats

Aamir

Khan

Hossain

et al. 2019

PeerJ

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BackgroundArjunolic acid (AA) is a potent phytochemical with wider pharmacological activities. Despite potential medicinal properties on various in vitro and in vivo studies, there is still a dearth of scientific data related to its safety profile and toxicological parameters. The current study aimed to investigate acute toxicity of AA in normal female Sprague Dawley rats.MethodsIn this study, AA was administered orally at an individual dose of 300 and 2000 mg/kg body weight to group 1 and 2 respectively, while group 3 served as normal control. All the animals were observed for 2 weeks to determine any behavioral and physical changes. On day 15, blood was collected for hematological and biochemical investigation, later animals from all the three groups were euthanized to harvest and store essential organs for histopathological analysis. Four different staining techniques; hematoxylin and eosin, Masson trichrome, Periodic acid Schiff and Oil O Red were used to investigate any alterations in different tissues through microscopical observation.ResultsThe results of the study showed no morbidity and mortality at two different dosage of AA treatment. Daily food & water intake, body weight, relative organ weight, hematological and biochemical parameters were detected to be normal with no severe alteration seen through microscopical investigation in the structure of harvested tissues. Our findings support the safety profile of AA, which was well tolerated at higher dose. Thus, an in-detail study on the subacute disease model is warranted.

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“…Hematological parameters RBC’s, WBC’s, platelets, hemoglobin, MCH, MCV, and % leukocyte count were determined as toxicity indices. 48 − 50 …”

Section: Methodsmentioning

confidence: 99%

Toxicological Screening of 4-Phenyl-3,4-dihydrobenzo[h]quinolin-2(1H)-one: A New Potential Candidate for Alzheimer’s Treatment

et al. 2021

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Toxicity studies are necessary for the development of a new drug. Naphthalene is a bicyclic molecule and is easy to derivatize. In our previous study, a derivative of naphthalene (4-phenyl,3,4-dihydrobenzoquinoline-2( H )one) was synthesized and reported its in vitro activity on different enzymes. This study was a probe to investigate the toxicity potential of that compound (SF3). Acute oral (425), subacute (407), and teratogenicity (414) studies were planned according to their respective guidelines given by organization of economic cooperation and development (OECD). Acute oral, subacute, and teratogenicity studies were carried out on 2000, 5–40, and 40 mg/kg doses. Blood samples were collected for hematological and biochemical analyses. Vital organs were excised for oxidative stress (superoxide dismutase, catalase, glutathione, and malondialdehyde) and histopathological analysis. LD 50 of SF3 was higher than 2000 mg/kg. In acute and subacute studies, levels of alkaline phosphates and aspartate transaminase were increased. Teratogenicity showed no resorptions, no skeletal or soft tissue abnormalities, and no cleft pallet. Oxidative stress biomarkers were close to the normal, and no increase in the malondialdehyde level was seen. Histopathological studies revealed normal tissue architecture of the selected organs, except kidney, in acute oral and subacute toxicity studies at 40 mg/kg. The study concluded that SF3 is safer if used as a drug.

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Acute and subacute toxicity evaluation of ethanol extract from aerial parts of Epigynum auritum in mice

Cited by 57 publications

References 33 publications

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Oral toxicity of arjunolic acid on hematological, biochemical and histopathological investigations in female Sprague Dawley rats

Toxicological Screening of 4-Phenyl-3,4-dihydrobenzo[h]quinolin-2(1H)-one: A New Potential Candidate for Alzheimer’s Treatment

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