In this study, the effects of different levels of aflatoxin B
1
(AFB
1
) on the growth performance, carcass traits, organ index, blood biochemistry, and antioxidant
capacity of yellow-feathered broilers were investigated to provide a reference for the application of AFB
1
-containing feed ingredients. In this test, yellow-feathered broilers
were chosen as the research objects and divided into five treatment groups, with seven replicates in each group and 75 broilers in each replicate. The AFB
1
concentration in the
diets of groups 1 to 5 were 1.5 μg/kg, 15 μg/kg, 30 μg/kg, 45 μg/kg, and 60 μg/kg, respectively. The results showed that when dietary AFB1 levels were greater than 45 μg/kg, the feed
conversion ratios of broilers of 1–21, 22–42, and 43–63 days of age increased (
P
<0.05). When dietary AFB
1
levels were 30 μg/kg, liver glutathione peroxidase
(GPx) activity was decreased (
P
<0.05), and serum transaminase (AST) activity was increased (
P
<0.05). Overall, dietary AFB
1
levels had
negative effects on growth performance, antioxidant capacity, blood biochemistry, and liver metabolism in yellow-feathered broilers. Based on using growth performance as the effect index,
AFB
1
levels in the diets of yellow-feathered broilers should not exceed 45 μg/kg. Based on using antioxidant capacity, liver function, and blood biochemistry as effect indexes,
AFB
1
levels in the diets of yellow-feathered broilers should not exceed 30 μg/kg.