Acute and sub-chronic oral toxicity study of purple sweet potato (Ipomoea batatas [L.] Lam) yogurt in mice (Mus musculus)

Abstract

Background and Aim: Food safety is an important aspect to be evaluated in preventing any potentially harmful side effects of food product such as yogurt. The purple sweet potato yogurt product was developed to combine the benefits of probiotic activities in yogurt and the bioactive effects of anthocyanin in purple sweet potato. This study was performed to investigate acute and sub-chronic oral toxicity of purple sweet potato yogurt (PSPY) in mice. Materials and Methods: Acute oral toxicity was evaluated by a 14-day observation for any clinical sign of toxicity on fifteen female balb/c mice following a single dosage of PSPY (nil, 2 or 5 g/kg body weight). The sub-chronic oral toxicity study was conducted by feeding PSPY to four groups of mice with the dose of 0, 12, 20, and 40 g/kg body weight for 28 days, and another group of mice receiving 40 g/kg body weight purple sweet potato for 14 days longer to observe any delayed toxicity effect. Body weight and clinical signs of toxicity were observed daily. Liver and kidney macroscopy and relative organ weight, liver histology, liver enzyme, and hematology profile analyses were done at the end of the study. Results: There were no signs of toxicity observed from the acute toxicity study and no abnormality in body weight, relative organ weight, and gross organ examination. In the sub-chronic toxicity study, there were no clinical signs of toxicity, no significant differences in body weight, relative liver weight, liver enzymes, hematology profile, or abnormality in gross and histological examination of the liver. Conclusion: This study shows that oral administration of PSPY in mice up to 5 g/kg body weight did not result in acute toxicity, while the dosage up to 40 g/kg body weight did not lead to sub-chronic toxicity.

Hematinic Potential of Olive Leaf Extract: Evidence from an In Vivo Study in Mice and a Pilot Study in Healthy Human Volunteers

“…Elevated serum creatinine is considered an indicator of renal toxicity [26]. Changes in liver and kidney weight (gain or loss) are important indicators of organ damage following exposure to toxic substances [27,28]. Additionally, splenomegaly, characterized by spleen enlargement, can arise due to hematologic abnormalities or infections [29].…”

Phytochemical component and toxicological evaluation of purple sweet potato leaf extract in male Sprague–Dawley rats

“…Any natural product containing bioactive molecules integrated into food is known as a functional food. In this context, one of the widely used functional food currently being studied is PSPL ( Khairani et al, 2022 ). PSPL, also known as Ipomoea batatas (L.) Lam.…”

Purple Sweet Potato Yogurt Affects Lipid Metabolism and Reduces Systemic Inflammation and Oxidative Stress in High Fat Diet Mice

“…(18) This research used purple sweet potato-based yogurt (PSPY) made from purple sweet potato puree mixed with low-fat milk and bacterial starter (Lactobacillus bulgaricus ATCC 11842, Lactobacillus acidophilus ATCC 4356, and Bifidobacterium longum), which reported to be safe to consume based on in vivo study. (19) Previous studies have shown that purple sweet potato can reduce carbohydrate content in yogurt and improve its sensory properties. (20) Nonetheless, the data in present-day about the potential protective effect of the product on anthropometry, lipid metabolism, oxidative stress, and pro-inflammatory markers is still inadequate.…”