Acute and Sub-acute Toxicity study of Amrtadi Churna

Mukhi, Sangeeta; Bose, Anindya; Das, Dilip Kumar; Panda, Sarat Kumar; Mohapatra, Debahuti; Latha, S Sai; Balaraman, Ashok Kumar

doi:10.52711/0974-360x.2021.00543

Cited by 4 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acute and subacute toxicity of Amrutadi churna , an Ayurvedic poly herbal formulation made of three herbs ( Tribulus terrestris , Emblica officinalis , Tinospora cordifolia ), was evaluated in rats. Toxicologically, when rats were even fed with higher dose of Churna (5000 mg/kg) it caused neither death nor severe toxicity in rats [ 59 ], which outlines the non-toxic nature of traditional herbs. Cisplatin toxicity was prevented in mice when T. terrestris fruit extract was administered orally at levels of up to 500 mg/kg body weight [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…Lately, T. terrestris hydroethanolic fruit extract, when dispensed at the rate of 100 mg/kg b.w., for 30 days, altered the negative effects on hematological parameters in male Wistar rats induced by acephate. Recently, hematology, serum biochemistry, and organ histology studies demonstrated that Amrutadi churna containing Emblica officinalis , T. terrestris , and Tinospora cordifolia did not cause hazardous effects at tested dose levels of 500 and 1000 mg/kg b.w, but it caused a mild hyperbilirubinemia with no liver damage [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive Compounds, Antioxidant, Anti-Inflammatory, Anti-Cancer, and Toxicity Assessment of Tribulus terrestris—In Vitro and In Vivo Studies

et al. 2022

View full text Add to dashboard Cite

Tribulus terrestris L. belongs to the family Zygophyllaceae and integral part of various ancient medicinal systems including Chinese, Indian, and European to combat various health ailments. The aim of the present study was to assess the phytochemical constituents, in vitro antioxidant activity using DPPH, FRAP, and H2O2 assays, in vitro anticancer activity using MTT assay, and in vitro and in vivo anti-inflammatory properties of T. terrestris. The acute and sub-acute toxicity of extracts exhibiting most biological potential was examined using murine models. Liquid–liquid partitioning followed by RP–HPLC sub-fraction of crude extract was performed. After that, ESI-MS/MS analysis was done for the timid identification of bioactive metabolites responsible for bioactivities of sub-fractions and HPLC analysis to quantify the compounds using external standards. Among all extracts, T. terrestris methanol extract was noted to hold maximum phenolic (341.3 mg GAE/g) and flavonoid (209 mg QE/g) contents, antioxidant activity in DPPH (IC50 71.4 µg/mL), FRAP (35.3 mmol/g), and H2O2 (65.3% inhibition) assays, anti-inflammatory activities in vitro at 400 µg/mL (heat-induced hemolysis, % inhibition 68.5; egg albumin denaturation, % inhibition 75.6%; serum albumin denaturation, % inhibition 80.2), and in vivo at 200 mg/kg (carrageenan-induced paw edema, % inhibition 69.3%; formaldehyde-induced paw edema, % inhibition 71.3%) and anticancer activity against breast cancer cell (MCF-7) proliferation (IC50 74.1 µg/mL). Acute and sub-acute toxicity studies recorded with no change in body weight, behavior, hematological, serum, and histopathological parameters in treated rats with T. terrestris methanol extracts when compared to control group. Fraction B obtained through liquid–liquid partitioning resulted in more bioactive potential as compared to the parent methanol extract. RP–HPLC analysis of fraction B resulted with four sub-fractions (TBTMF1-TBTMF4), wherein TBTMF3 delineated notable bioactive capabilities as compared to other fractions and parent methanol extract. ESI-MS/MS analysis of TBTMF3 resulted with tentative identification of myricetin, rutin, liquitrigenin, physcion, and protodioscin. It can be stated that T. terrestris is a potential bearing herb and findings of current study further verify the claims made in ancient medicinal systems. However, after investigation of each identified compound, it must be considered for drug discovery.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioactive Compounds, Antioxidant, Anti-Inflammatory, Anti-Cancer, and Toxicity Assessment of Tribulus terrestris—In Vitro and In Vivo Studies

et al. 2022

View full text Add to dashboard Cite

show abstract

Acute and Subacute Oral Toxicity studies on Aquatic plant Trapa natans L. using a Rat Model

Majee

Mazumder

Choudhary

2022

RJPT

View full text Add to dashboard Cite

Trapa natans L. is annual aquatic plant generally kwon as Water caltrp, Water chest nut belonging to the Trapaceae or Lytraceae family. Trapa natans L. is use for the treatment of wide no of disease with out proper toxicity study. The present study was designed to evaluate the acute and sub-acute toxicities of Trapa natans L following the OECD guidelines. In acute oral toxicity, the plant extract was administered orally at the doses of 300 mg/kg, 1000 mg/kg, 2000 mg/kg, and 4000 mg/kg. After the dosing, all animals were kept in close observation for 14 days. In the case of the subacute toxicity study, the total number of animals was divided into four groups, each having ten animals, 5 males, and 5 females in each group. Group I was represented as the control, administered orally with aqueous tween 80 solution 2% (v/v), and group II to group IV received plant extract of doses 400, 600, and 1000 mg/kg, respectively, once a day for 28 days. From the acute and subacute toxicity studies, no sign of toxicity was observed. In the sub-acute toxicity study, no major changes were noted on the biochemical parameters and hematological analysis. The experimental results of the toxicity studies suggested that the LD50 values of the Trapa natans L extract were more than 4000mg/kg and thus, the drug was found to be absolutely safe and nontoxic.

show abstract

Acute and Subacute Toxicity Studies of Ethanol, Chloroform extracts and Flower oil of Senecio edgeworthii hook plant

Shende

Kakadiya

2023

RJPT

View full text Add to dashboard Cite

This study investigated the acute and subacute oral toxicity of SE- Oil, SE-Alcohol and SE-Chloro extracts of Senecio edgeworthii (SE) in a rodent for evaluating its safety profile. Senecio edgeworthii (SE) plant for the acute oral toxicity (300 to 5000mg/kg) and subacute oral toxicity SE- Oil (SE-Oil 50, 200, 500mg/kg) and SE-Chloro (SEC 100, 500, 1000mg/kg) and SE-Alcohol (SEA 100, 500, 1000mg/kg) studies were administered orally according to the guidelines 423 and 407 of OECD, respectively. In the acute oral toxicity study, doses are administered by 300mg/kg, 1000mg/kg, 2000mg/kg and 5000mg/kg (OECD guideline no. 423). SEA and SEC do not show toxicity up to 5000mg/kg; SE- Oil shows toxicity at a dose of 2500mg/kg. In the subacute toxicity study, the daily oral treatment with extracts of SEA and SEC 100, 500, 1000mg/kg, SE-Oil 50, 250, 500mg/kg of extracts for 28 days did not produce any death or hazard. Likewise, SEA and SEC 100, 500, 1000mg/kg, SE-Oil 50, 250mg/kg of Senecio edgeworthii hook no significant changes were recorded in food intake, body weight gain, the biochemical and haematological parameter of control and treated rats. SE-Oil 500mg/kg of significant changes were recorded in food intake and body weight gains are decreased compared to the control group and SE-Oil 500mg/kg there was a significant (P < 0.05) decrease in RBC counts in the treated animals compared to that of the control group counts in the treated animals and induction (ALT, AST, and alkaline phosphatase and Total protein). SE- Oil 500mg/kg of extract treaded group saw mild liver pathological findings characterized by abnormal hepatic configuration was observed with treated animals. The present result from the acute and subacute toxicity study Senecio edgeworthii hook alcohol and chloroform extract is considered relatively safe on acute and subacute oral exposure. Senecio edgeworthii hook flower hexane extract shows acute toxicity at dose 2500mg/kg and in subacute toxicity study, higher dose 500mg/kg.

show abstract

Acute and Sub-acute Toxicity study of Amrtadi Churna

Cited by 4 publications

References 26 publications

Bioactive Compounds, Antioxidant, Anti-Inflammatory, Anti-Cancer, and Toxicity Assessment of Tribulus terrestris—In Vitro and In Vivo Studies

Bioactive Compounds, Antioxidant, Anti-Inflammatory, Anti-Cancer, and Toxicity Assessment of Tribulus terrestris—In Vitro and In Vivo Studies

Acute and Subacute Oral Toxicity studies on Aquatic plant Trapa natans L. using a Rat Model

Acute and Subacute Toxicity Studies of Ethanol, Chloroform extracts and Flower oil of Senecio edgeworthii hook plant

Contact Info

Product

Resources

About