Acute and latent adenovirus in COPD

McManus, Terence; Marley, Anne-Marie; Baxter, Noreen; Christie, Sharon; Elborn, J. Stuart; Heaney, Liam G.; Coyle, Peter; Kidney, Joseph C.

doi:10.1016/j.rmed.2007.05.015

Cited by 26 publications

(31 citation statements)

References 26 publications

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“…The lysis and death of lytically infected cells are accelerated by the adenovirus death protein (ADP) (2,20). However, species C adenoviruses are also able to infect some lymphocytic and macrophage cell lines without proceeding through a lytic infection (11,13,21,22). We recently found that adenovirus types 2 and 5 are able to persist in several lymphocytic cell lines for over a year in culture (8).…”

Section: Discussionmentioning

confidence: 99%

Adenovirus Death Protein (ADP) Is Required for Lytic Infection of Human Lymphocytes

Murali

Ornelles

Gooding

et al. 2014

J Virol

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The adenovirus death protein (ADP) is expressed at late times during a lytic infection of species C adenoviruses. ADP promotes the release of progeny virus by accelerating the lysis and death of the host cell. Since some human lymphocytes survive while maintaining a persistent infection with species C adenovirus, we compared ADP expression in these cells with ADP expression in lymphocytes that proceed with a lytic infection. Levels of ADP were low in KE37 and BJAB cells, which support a persistent infection. In contrast, levels of ADP mRNA and protein were higher in Jurkat cells, which proceed with a lytic infection. Epithelial cells infected with an ADP-overexpressing virus died more quickly than epithelial cells infected with an ADP-deleted virus. However, KE37, and BJAB cells remained viable after infection with the ADP-overexpressing virus. Although the levels of ADP mRNA increased in KE37 and BJAB cells infected with the ADP-overexpressing virus, the fraction of cells with detectable ADP was unchanged, suggesting that the control of ADP expression differs between epithelial and lymphocytic cells. When infected with an ADP-deleted adenovirus, Jurkat cells survived and maintained viral DNA for greater than 1 month. These findings are consistent with the notion that the level of ADP expression determines whether lymphocytic cells proceed with a lytic or a persistent adenovirus infection.

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Section: Discussionmentioning

confidence: 99%

Adenovirus Death Protein (ADP) Is Required for Lytic Infection of Human Lymphocytes

Murali

Ornelles

Gooding

et al. 2014

J Virol

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“…Higher RSV detection rates were observed in samples from patients with COPD-AE and those in a stable condition, compared with rates reported in the literature (5)(6)(7)(19)(20)(21)(22)(23)(24). This may be explained by the different methodologies and specimen usage.…”

Section: Discussionmentioning

confidence: 55%

“…However, the frequency of virus-induced COPD-AE cases was found to be higher than previously assumed, as a result of more sensitive molecular detection methods such, as PCR (19)(20)(21). In previous studies, adenovirus was detected in ~1% of COPD-AE cases (19)(20)(21). The percentage of patients with COPD-AE who are infected with RSV varies from 2.3-28% (5)(6)(7)20,(22)(23)(24).…”

Section: Discussionmentioning

confidence: 84%

“…In guinea pigs, cigarette smoke-induced emphysema is increased in response to latent adenoviral infection (8). By contrast, McManus et al (19) detected the adenovirus hexon gene and 5 E1A DNA and mRNA in a low number of sputum samples from patients with COPD-AE and in stable conditions. In accordance with those of previous studies, the results of the present study suggested low adenovirus detection in patients with COPD-AE and those in a stable condition.…”

Section: Discussionmentioning

confidence: 95%

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Detection of adenovirus and respiratory syncytial virus in patients with chronic obstructive pulmonary disease: Exacerbation versus stable condition

Köktürk

Bozdayı

Yılmaz

et al. 2015

Molecular Medicine Reports

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Abstract. Latent infection with adenovirus and respiratory syncytial virus (RSV) is associated with chronic obstructive pulmonary disease (COPD). The role of respiratory viral infections are emerging in COPD exacerbations. The present study aimed to investigate the prevalence of adenovirus and RSV serotypes A and B in individuals with acute exacerbations of COPD (COPD-AE) and stable COPD. Twenty seven patients with COPD-AE were evaluated using a prospective longitudinal study design. Induced sputum, sera and nasal smears were sampled from patients experiencing COPD-AE and those in a stable condition. Adenoplex ® multiplex polymerase chain reaction (PCR) kits and Invitek RTP ® DNA/RNA Virus Mini kits were used for PCR assays of adenovirus and RSV, respectively. Eighteen patients who experienced a COPD-AE were also evaluated while in a stable condition. The results showed that three sputum samples were positive for adenovirus in patients experiencing an exacerbation, while one was positive among the patients in a stable condition. RSV serotype A was detected in 17/27 (63%) patients with COPD-AE and 10/18 (55.6%) patients in a stable condition. RSV serotype B was not detected. Patients with COPD-AE, who were positive for RSV serotype A exhibited higher serum fibrinogen levels than those who were negative (438.60±126.08 mg/dl compared with 287.60±85.91 mg/dl; P=0.004). Eight/ten patients who were positive for RSV serotype A while in a stable condition, were also positive during COPD-AE. The results of the present study suggested that RSV infection may be prevalent in patients with COPD-AE and in those in a stable condition. Therefore, chronic RSV infection may occur in COPD. The detection and prevention of RSV may be useful in the management of COPD.

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“…Seit längerem wird angenommen, dass Adenovi− ren zu latenten Infektionen bei Patienten mit COPD führen [27] und die chronische Entzündung verstärken [28]. Aktuelle epide− miologische Daten weisen jedoch darauf hin, dass dieses Phäno− men relativ selten zu beobachten ist [29]. Insgesamt zeigen die− se Daten aber, dass Atemwegsviren auch eine Rolle bei dem Ver− lauf der Erkrankung spielen.…”

Section: Atemwegsviren Bei Stabiler Copdunclassified