Acute and joint toxicity of twelve substituted benzene compounds to Propsilocerus akamusi Tokunaga

Cao, Chuanwang; Niu, Fang; Li, Xiaopeng; Ge, Shilin; Wang, Zhiying

doi:10.2478/s11535-014-0289-y

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…Given this, additivity between these chemicals is reasonable. This combining result was also reported by many other researchers using various indicator species and physiological effects. − …”

Section: Resultssupporting

confidence: 87%

“…The toxicity order of benzenes calculated form mobility inhibition is nitrobenzene ≈ chlorobenzene > methylbenzene > benzene. This order was also reported by many other researchers using different ecological trait. − These results showed that atomic H of benzene substituted by different groups resulted in different toxicity because benzene ring structures changed by different groups produced different toxic mechanisms. The toxicities of groups are in decreasing order of −NO 2 > −Cl > −CH 3 > −H.…”

Section: Resultssupporting

confidence: 77%

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Development of Microfluidic Dilution Network-Based System for Lab-on-a-Chip Microalgal Bioassays

et al. 2018

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Because of the crucial ecological significance of microalgae, microalgal bioassays have become one of the most demanding tests from all classic aquatic toxicity tests in regulatory frameworks. However, conventional algal tests tend to be lab-intensive and time-and space-consuming, and they have not been utilized to their full potential for routine toxicity assessments. Microfluidics should be a user-friendly alternative. Particularly, dilution to generate gradients that are appropriate for screening experiments can be precisely attained by microfluidic network in a simple and cost-/time-/space-saving way. Here, we demonstrate a microfluidics series toward routine microalgal bioassays, including pretest, single, and joint toxicity test. The chip mainly consists of upstream dilution network (single serial dilution module (logarithmic/linear gradient generator) or multiple (binary/ternary/quaternary) mixing serial dilution module) and downstream diffusible culturing module. It allows the processes of chemical liquid dilution and diffusion, microscale microalgal culture, cell stimulation, and online screening to be integrated into a single device. Electric theorems with the aid of EDA (electronic design automation) simulation were innovatively introduced to minimize design effort for such systems. Using the device, microalgae were successfully cultured and stressed on-chip. The simple assay provides multibiological trait assessments of cell division rate, autofluorescence, esterase activity, and mobile capacity. This work showed promise in developing a high-throughput microfluidic platform for microalgal bioassays as well as lab-on-a-chip screening experiments in the cell-based quantitative assessment of environmental health risks.

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Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 77%

Development of Microfluidic Dilution Network-Based System for Lab-on-a-Chip Microalgal Bioassays

et al. 2018

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“…Stolte et al [29] also observed that the toxicity of imidazolium cation on environmental microorganisms increased with the increase of the length of its side chain. For [Bzmim] + , the strong inhibition of cell growth might be due to the toxicity of the benzyl group to microorganisms [30]. The phenomenon that [OAc] − was more toxic than Cl − was consistent with the results reported by other researchers [12, 17].…”

Section: Resultssupporting

confidence: 75%

Mechanistic insights into the effect of imidazolium ionic liquid on lipid production by Geotrichum fermentans

Liu

Zong

Linhardt

et al. 2016

Biotechnol Biofuels

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BackgroundIonic liquid (IL) pretreatment has emerged as a promising technique that enables complete utilization of lignocellulosic biomass for biofuel production. However, imidazolium IL has recently been shown to exhibit inhibitory effect on cell growth and product formation of industrial microbes, such as oleaginous microorganisms. To date, the mechanism of this inhibition remains largely unknown.ResultsIn this study, the feasibility of [Bmim][OAc]-pretreated rice straw hydrolysate as a substrate for microbial lipid production by Geotrichum fermentans, also known as Trichosporon fermentans, was evaluated. The residual [Bmim][OAc] present in the hydrolysate caused a reduction in biomass and lipid content (43.6 and 28.1%, respectively) of G. fermentans, compared with those of the control (7.8 g/L and 52.6%, respectively). Seven imidazolium ILs, [Emim][DEP], [Emim]Cl, [Amim]Cl, [Bmim]Cl, [Bzmim]Cl, [Emim][OAc], and [Bmim][OAc], capable of efficient pretreatment of lignocellulosic biomass were tested for their effects on the cell growth and lipid accumulation of G. fermentans to better understand the impact of imidazolium IL on the lipid production. All the ILs tested inhibited the cell growth and lipid accumulation. In addition, both the cation and the anion of IL contributed to IL toxicity. The side chain of IL cations showed a clear impact on toxicity. On examining IL anions, [OAc]− was found to be more toxic than those of [DEP]− and Cl−. IL exhibited its toxicity by inhibiting sugar consumption and key enzyme (malic enzyme and ATP-citrate lyase) activities of G. fermentans. Cell membrane permeability was also altered to different extents in the presence of various ILs. Scanning electron microscopy revealed that IL induces fibrous structure on the surface of G. fermentans cell, which might represent an adaptive mechanism of the yeast to IL.ConclusionsThis work gives some mechanistic insights into the impact of imidazolium IL on the cell growth and lipid accumulation of oleaginous yeast, which is important for IL integration in lignocellulosic biofuel production, especially for microbial lipid production.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0682-z) contains supplementary material, which is available to authorized users.

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“…According to the combined effects of TDTs on zebrafish hatching, mortality and malformation rates, two methods were used to evaluate the combined toxicity of TDT: Toxic Unit (TU) and Mixture Toxicity Index (MTI), the interactions among TDTs were shown to be partly additive toxicological effect (Cao et al, 2014). The 120-hpf LC 50 and EC 50 values for the TDT mixture (TCSþ2,4,6-TCPþ2,4-DCP) were 2.28 and 1.16 mg/L, respectively ( Supplementary Fig.…”

Section: Effects Of Tdt On Behavior Of Zebrafish Embryos and Larvaementioning

confidence: 99%

Response mechanisms to joint exposure of triclosan and its chlorinated derivatives on zebrafish (Danio rerio) behavior

et al. 2018

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h i g h l i g h t sJoint toxicity of triclosan and its main products (TDTs) to zebrafish behavior. TDTs-stress produced larval abnormality and adult anxiety-or autism-like behavior. Behavioral effects focus on social interaction, preference, T-maze tests and so on. Abnormal behavior from changes in the related gene, biomarker and pathological tissue. TDTs produced vascular ablation in head and occurrence of massive apoptosis in brain. a r t i c l e i n f o b s t r a c tTriclosan (TCS), 2,4,6-trichlorophenol (2,4,6-TCP) and 2,4-dichlorophenol (2,4-DCP) frequently co-exist in real-world aquatic environments; the latter two contaminants contributing to TCS photolytic products or chlorinated derivatives. There is a paucity of information regarding their joint toxicity to aquatic organisms leading us to study their effects on the swimming behavior of zebrafish (Danio rerio). Herein, we reported that 0.28 mg/L TDT exposure (mixtures of TCS, 2,4,6-TCP and 2,4-DCP) enhanced 24-hpf embryonic spontaneous movement frequency, 96-hpf larval activity; however, the 0.56 and 1.12 mg/L TDT treatments decreased all of these behavioral endpoints. All adult behavioral tests demonstrated that chronic TDT exposure (0.14 mg/L) led to hyperactivity and restlessness in adult zebrafish. A 0.14 mg/L TD DATE /@ "M/d/yyyy" 11/21/2017T treatment led to anxiety-like behavior in a bottom dwelling test and excessive panic and low hedging capacity in a conditioned place preference test. Social interaction test demonstrated that zebrafish preferred quiet and isolated space in response to TDT stress. Zebrafish memory was significantly decreased in a T-maze experiment. Whole mount in situ hybridization of pax2a and bcl2l11 genes revealed that their differential expression in the brain and skeleton were related to the corresponding phenotypic behavioral abnormality. A series of biomarker and estrogen receptor assays demonstrated that TDT acute exposure caused abnormal energy metabolism and neurological diseases. AO staining revealed that TDT exposure produced vascular ablation in the head, as well as the occurrence of massive apoptosis in the brain. TEM observation showed pyknosis of nucleus following TDT exposure. These results allow assessment of mechanisms for zebrafish abnormal behavior in response to TDT exposure, and are useful for early intervention and gene therapy of contaminant-induced diseases.

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Acute and joint toxicity of twelve substituted benzene compounds to Propsilocerus akamusi Tokunaga

Cited by 7 publications

References 20 publications

Development of Microfluidic Dilution Network-Based System for Lab-on-a-Chip Microalgal Bioassays

Development of Microfluidic Dilution Network-Based System for Lab-on-a-Chip Microalgal Bioassays

Mechanistic insights into the effect of imidazolium ionic liquid on lipid production by Geotrichum fermentans

Response mechanisms to joint exposure of triclosan and its chlorinated derivatives on zebrafish (Danio rerio) behavior

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