Acute and Chronic Toxicity Studies of the Water and Ethanol Extracts of Natural and Callus Cultures of Munronia pinnata in Healthy Wistar Rats

Hapuarachchi, SD; Suresh, T.S.; Senerath, Wtpsk

doi:10.1055/s-0033-1336522

Cited by 3 publications

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“…This was in accordance with the method of preparing decoctions in traditional medicine. 18 The extracts were freeze-dried and aliquots were stored at –20°C until use.…”

Section: Methodsmentioning

confidence: 99%

“…flavonoids); [11][12][13] ii) there are documented antiviral effects against other viruses; 14,15 iii) their use has been found to be safe in children and pregnant women; and iv) there are documented hepatoprotective effects. 16,17 The present study aimed to investigate the anti-viral activity of aqueous extracts of MP, PG and AM against all four serotypes of dengue virus in Vero cell cultures. All freshly collected plant materials and dried flowers were washed thoroughly in running tap water.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava

et al. 2023

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Dengue is an arboviral (insect-transmitted) infection of global concern. Currently, there are still no specific dengue antiviral agents to treat the disease. Plant extracts have been used in traditional medicine for treating various viral infections — thus, in the present study, aqueous extracts of dried flowers of Aegle marmelos (AM), whole plant of Munronia pinnata (MP) and leaves of Psidium guajava (PG) were investigated for their potential capacity to inhibit dengue virus infection of Vero cells. The maximum non-toxic dose (MNTD) and the 50% cytotoxic concentration (CC50) were determined by using the MTT assay. A plaque reduction antiviral assay was carried out with dengue virus types 1 (DV1), 2 (DV2), 3 (DV3) and 4 (DV4), in order to calculate the half-maximum inhibitory concentration (IC50). AM extract inhibited all four virus serotypes tested; MP extract inhibited DV1, DV2 and DV4, but not DV3; PG extract inhibited DV1, DV2 and DV4, but not DV3. Thus, the results suggest that AM is a promising candidate for the pan-serotype inhibition of dengue viral activity.

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“…This was in accordance with the method of preparing decoctions in traditional medicine. 18 The extracts were freeze-dried and aliquots were stored at –20°C until use.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava

et al. 2023

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“…pinnata is rich in phytochemicals such as phytosterols, fatty acids, sesquiterpenes (Napagoda et al, 2014), and limonoids (Yang et al, 2019), saponins, alkaloids, tannins, flavonoids, steroids and glycosides (Dharmadasa et al, 2013). Previous studies report that M. pinnata has hypoglycemic (Hapuarachchi et al, 2011), antioxidant, antiinflammatory (Hapuarachchi et al, 2015), antibacterial (Keerawelle et al, 2019), antifungal (Kaliyadasa et al, 2020), antimalarial (Dharmadasa et al, 2012), and hepatoprotective (Hapuarachchi et al, 2013) effects.…”

Section: Introductionmentioning

confidence: 99%

“…In Sri Lankan traditional medicine, Munronia pinnata (Family: Meliaceae; Common name: ground neem/king bitter; Local name: Bin Kohomba,) is used as a treatment for fever, purification of blood, dysentery, diabetes, tuberculosis, cough, stomachache, sores, malaria, skin diseases (Hapuarachchi et al, 2013) and brain, lung cancers (Kuruppu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Study on the Anti-dengue Viral Activity of Munronia pinnata

et al. 2022

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Dengue infection is a major health care problem in tropical and subtropical countries. The recently approved dengue vaccine has limitations, and there is no antiviral drug for treatment at present.For centuries plants and plant extracts have been used in traditional medicine for the treatment of various infections. The whole plant of Munronia pinnata, which has been used for treating fever patients in Sri Lankan traditional medicine, was tested for anti-dengue viral activity.The cytotoxicity assay of M. pinnata on Vero cells using 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) revealed Maximum Nontoxic Dose (MNTD) as 125 μg/ml and 50% cytotoxic concentration (CC50) as 428.9 ± 21.55 μg/ml. Plaque reduction antiviral assay performed on dengue-4 virus infected Vero cells demonstrated half-maximal inhibitory concentration (IC50) as 26.12 ± 0.91 μg/ml. The selectivity index (SI) of Dengue infection is a major health care problem in tropical and subtropical countries. The recently approved dengue vaccine has limitations, and there is no antiviral drug for treatment at present.For centuries plants and plant extracts have been used in traditional medicine for the treatment of various infections. The whole plant of Munronia pinnata, which has been used for treating fever patients in Sri Lankan traditional medicine, was tested for anti-dengue viral activity.The cytotoxicity assay of M. pinnata on Vero cells using 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) revealed Maximum Nontoxic Dose (MNTD) as 125 μg/ml and 50% cytotoxic concentration (CC50) as 428.9 ± 21.55 μg/ml. Plaque reduction antiviral assay performed on dengue-4 virus infected Vero cells demonstrated half-maximal inhibitory concentration (IC50) as 26.12 ± 0.91 μg/ml. The selectivity index (SI) of M. pinnata was 16.42.Based on the selectivity index, Munronia pinnata appears to be a viable candidate for identifying biologically active compounds with anti-dengue viral activity.Keywords: Dengue, Munronia pinnata, Antiviral, Plaque reduction Assay, Vero Cells was 16.42.Based on the selectivity index, Munronia pinnata appears to be a viable candidate for identifying biologically active compounds with anti-dengue viral activity. Keywords: Dengue, Munronia pinnata, Antiviral, Plaque reduction Assay, Vero Cells

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Munronia pinnata (Wall.) Theob.: Unveiling phytochemistry and dual inhibition of 5-lipoxygenase and microsomal prostaglandin E2 synthase (mPGES)-1

Napagoda

Gerstmeier

Koeberle

et al. 2014

Journal of Ethnopharmacology

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Acute and Chronic Toxicity Studies of the Water and Ethanol Extracts of Natural and Callus Cultures of Munronia pinnata in Healthy Wistar Rats

Cited by 3 publications

References 0 publications

In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava

In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava

A Preliminary Study on the Anti-dengue Viral Activity of Munronia pinnata

Munronia pinnata (Wall.) Theob.: Unveiling phytochemistry and dual inhibition of 5-lipoxygenase and microsomal prostaglandin E2 synthase (mPGES)-1

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