Acute and chronic toxicity of pesticides on tadpoles of Physalaemus cuvieri (Anura, Leptodactylidae)

Wrubleswski, Juliana; Reichert, Francisco Wilson; Galon, Leandro; Hartmann, Paulo Afonso; Hartmann, Marília Teresinha

doi:10.1007/s10646-018-1900-1

Cited by 26 publications

(7 citation statements)

References 47 publications

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“…Its population stability and abundance in the areas that occur (Frost 2017), good adaptability in the laboratory, and early biological response justify the species’ choice (Herek et al, 2020; Araújo et al, 2020ab; Rutkoski et al, 2020). Previous studies using this species report the effects of water pollution caused by wastewater runoff (Wrubleswski et al 2018). Therefore, in this study, we selected P. cuvieri as our choice of a translational model vertebrate.…”

Section: Introductionmentioning

confidence: 99%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles / 5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulatedwith these peptides during 24 hours of exposure in two concentrations (100 and 500 ng/mL). The control group (“C”) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and neurotoxicity activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has neurotoxics effects in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.HIGHLIGHTSSARS-CoV-2 spike protein peptides (PSDP) were synthesized, purified, and characterized by solid phase peptide synthesis.PSDP peptides promoted REDOX imbalance and acute neurotoxicity in tadpoles (Physalaemus cuvieri)In silico studies have shown interactionsbetween peptides and acetylcholinesterase and antioxidant enzymesAquatic particle contamination of SARS-CoV-2 can constitute additional environmental damageGRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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“…Like the other fungicides analyzed in the present study, Brazilian National Environment Council (CONAMA) resolution 357/2005 does not establish a maximum threshold for the use of tebuconazol. Wrubleswski et al (2018) tested tebuconazol in P. cuvieri at both chronic (0.11, 0.02, and 0.01 mg/L) and acute median lethal concentration (LC50) concentrations, and recorded mortality in all cases, with the onset of mortality in the acute test occurring in the first 24 h at a concentration of 0.11 mg/L.…”

Section: Discussionmentioning

confidence: 99%

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

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Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

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“…Teratogenic and neurotoxic effects were observed in R. arenarum embryos exposed for 24 h to 15 and 20 mg L −1 , respectively (Svartz et al, 2012). In tadpoles of P. cuvieri, NOEC and LOEC values of 24 and 48 mg L −1 , respectively, were reported for natatorium behavior (Wrubleswski et al, 2018). Sublethal effects were studied on the native reptile Caiman latirostris.…”

Section: Adverse Effects Of Atz On Vertebratesmentioning

confidence: 99%

Atrazine characterization: An update on uses, monitoring, effects, and environmental impact, for the development of regulatory policies in Argentina

Gagnetén

Regaldo

Carriquiriborde

et al. 2022

Integr Envir Assess & Manag

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Atrazine (ATZ) is the third most widely used herbicide in Argentina (10 000 t year−1) and is approved for sugar cane, flax, corn, sorghum, and tea. An assessment of the ATZ environmental impacts was conducted at the request of the Ministry of Environment and Sustainable Development of Argentina. A review of 541 national and international technical and scientific reports and a survey among agricultural technicians, applicators, and producers was done. The survey revealed that 94% of ATZ applications are terrestrial and use diversion exists, associated mainly with soybean cultivation. Atrazine was reported at high frequencies (50%–100%) in surface and groundwater, sediments, and soils, sometimes exceeding permitted limits. Several sublethal effects induced by ATZ on invertebrate and vertebrate species were found, sometimes at concentrations lower than those in water quality guidelines (<3 µg L−1) or the environmental concentrations found in Argentina. Available epidemiological or human health studies of local populations are extremely scarce. This assessment also demonstrated that herbicides are ubiquitous in the environment. The investigation highlights the need for further studies assessing the adverse effects of ATZ on local species, ecosystems, and human health. Therefore, the precautionary principle is recommended to promote better application standards and product traceability to reduce volumes entering the environment and to avoid use deviation. In addition, this work concluded that there is a need for reviewing the toxicological classification, establishing buffer zones for ATZ application, introducing specific management guidelines, and expanding local studies of toxicity, ecotoxicity, and human epidemiology for environmental and health risk assessments. This study could also serve as a preliminary risk evaluation for establishing a final regulatory action and for considering ATZ inclusion in Annex III of the Rotterdam Convention. Finally, the requirements to consider its inclusion in Annex A (Elimination) or B (Restriction) of the Stockholm Convention were evaluated and discussed, and information on the potential of long‐range transport was the only criterion with no information to consider. Integr Environ Assess Manag 2023;19:684–697. © 2022 SETAC

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Acute and chronic toxicity of pesticides on tadpoles of Physalaemus cuvieri (Anura, Leptodactylidae)

Cited by 26 publications

References 47 publications

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Atrazine characterization: An update on uses, monitoring, effects, and environmental impact, for the development of regulatory policies in Argentina

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