Acute and chronic hypoxia affects HIF-1α mRNA levels in sea bass (Dicentrarchus labrax)

Terova, Genciana; Rimoldi, Simona; Corà, Samuela; Bernardini, Giovanni; Gornati, Rosalba; Saroglia, Marco

doi:10.1016/j.aquaculture.2008.03.041

Cited by 143 publications

(83 citation statements)

References 44 publications

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“…This expression pattern was consistent with the studies in European seabass, Wuchang bream ( Megalobrama amblycephala Yih, 1955) and Chinese sucker (Myxocyprinus asiaticus) (Terova et al 2008, Shen et al 2010, Chen et al 2012. Unlike HIF-1α in these three teleost, HIF-1α of grass carp was highly expressed in the eyes and kidneys (Law et al 2006).…”

Section: Expression Analysis Of Hif-1α Insupporting

confidence: 79%

Molecular Cloning and Expression Analysis of Hypoxia Inducible Factor 1α in Tongue Sole, <I>Cynoglossus Semilaevis</I> (Actinopterygii: Pleuronectiformes: Cynoglossidae), Subjected to Acute Hypoxia

Qi¹,

Wang²,

Zhang³

et al. 2013

Acta Icth et Piscat

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Section: Expression Analysis Of Hif-1α Insupporting

confidence: 79%

Molecular Cloning and Expression Analysis of Hypoxia Inducible Factor 1α in Tongue Sole, <I>Cynoglossus Semilaevis</I> (Actinopterygii: Pleuronectiformes: Cynoglossidae), Subjected to Acute Hypoxia

Qi¹,

Wang²,

Zhang³

et al. 2013

Acta Icth et Piscat

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“…Furthermore, although indices such as WB cortisol and glucose have been shown to be effective in measuring the acute and chronic stress response of a variety of small fishes, including sticklebacks (Reubush and Heath 1996;Scarabello et al 1992;King and Berlinsky 2006;Pottinger et al 2002) these are relatively crude measures of a complex biological response. Future investigations may benefit from a more refined approach to the assessment of HPI activity, including repeat measures in individual fish by the collection of water-borne cortisol and quantification of transcription factors such as hypoxia inducible factor-1, that are known to be associated with regulating the expression of physiologically relevant genes (Terova et al 2008). Results from the control fish in this study also highlighted the existence of between-population differences in corticosteroid and metabolite concentrations that appeared to ameliorate over the time fish were in the laboratory.…”

Section: Discussionmentioning

confidence: 99%

The effects of acute and chronic hypoxia on cortisol, glucose and lactate concentrations in different populations of three-spined stickleback

O’Connor

Pottinger

Sneddon

2010

Fish Physiol Biochem

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Article (refereed)O'Connor, E.A.; Pottinger, T.G.; Sneddon, L.U.. 2011 The effects of acute and chronic hypoxia on cortisol, glucose and lactate concentrations in different populations of three-spined stickleback. Fish Physiology and Biochemistry, 37 (3). 461-469. 10.1007/s10695-010-9447-y Contact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trade marks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. ABSTRACTThe response of individuals from three different populations of three-spined sticklebacks to acute and chronic periods of hypoxia (4.4 kPa DO, 2.2 mg l -1 ) were tested using measures of whole-body (WB) cortisol, glucose and lactate. Although there was no evidence of a neuroendocrine stress response to acute hypoxia, fish from the population least likely to experience hypoxia in their native habitat had the largest response to low oxygen, with significant evidence of anaerobic glycolysis after two hours of hypoxia. However, there was no measurable effect of a more prolonged period (seven days) of hypoxia on any of the fish in this study, suggesting that they acclimated to this low level of oxygen over time. Between-population differences in the analytes tested were observed in the control fish of the acute hypoxia trial, which had been in the laboratory for 16 days. However, these differences were not apparent among the control fish in the chronic exposure groups that had been held in the laboratory for 23 days suggesting that these site-specific trends in physiological status were acclimatory. Overall, the results of this study suggest that local environmental conditions may shape sticklebacks' general physiological profile as well as influencing their response to hypoxia. 2 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 Reduced dissolved oxygen (DO) in the water is an important environmental stressor for fish. Periods of low DO, or hypoxia, can occur as a result of many factors such as eutrophication, elevated ambient temperature and algal blooms. With climate-related warming of freshwater bodies predicted to continue (Bates et al. 2008;Johnson et al. 2009), a greater understanding of the response of fish species to consequent environmental factors such as hypoxia could prove beneficial (Gitay et al. 2002). The three-spined stickleback, Gasterosteus aculeatus, is a highly adaptable teleost fish, ubiquitous throughout temperate regions of the Northern hemisphere. Their small size, widedistribution, short generation time and ease of care in captivity have made them a popular study species across a number of different disciplines including ecology, toxicology and molecular biology (Barber and Nettleship 2010; Katsiadaki et al. 2007;Colosimo et al. 2005). However, relatively little is known about how these fish respond to hypoxic stress.Previous studies indicate that the three-...

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“…The physiological response (i.e., cortisol, glucose, and haematocrit) is similar in fish kept under hypoxic (3-4.5 mg O 2 /L) and normoxic (7.4 mg/L) conditions [17]. Terova et al [18] observed that acute hypoxia occurs at 1.9 mg/L after 4 hrs of exposure and that chronic hypoxia occurs at 4.3 mg/L after 15 days of exposure. According to Pichavant et al [19], chronic hypoxia had no significant difference in haematocrit, erythrocyte number or mean corpuscular haemoglobin concentration in sea bass.…”

Section: Cages Oxygenationmentioning

confidence: 99%

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

et al. 2016

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An air diffusion based system (Airx) was developed to control the dissolved oxygen levels in aquaculture sea cages. The system was introduced and then tested for 37 days in a sea bass sea cage (aerated cage). A second sea bass sea cage, without the AirX, was used as a control. Oxygen levels were measured in both cages at the start of the trial, before the AirX system was introduced, and during the working period of the AirX system. Fish samples were collected 15 days after the AirX system was introduced and at the end of the experiment. Blood smears were prepared and examined microscopically. Erythrocyte major axis, minor axis and area of fish erythrocytes were measured. Leucocyte differentiation was also examined. In the control cage, the fish had significantly larger red blood cells when compared with the red blood cells of the fish in the aerated cage. Histological examination of the gills and brain revealed no morphological differences or alterations between the two groups of fish. This study demonstrated that an air diffuser system could improve the water quality of fish farmed in sea cages and enhance sea bass physiological performance, especially if DO levels fall below 60% oxygen saturation.

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Acute and chronic hypoxia affects HIF-1α mRNA levels in sea bass (Dicentrarchus labrax)

Cited by 143 publications

References 44 publications

Molecular Cloning and Expression Analysis of Hypoxia Inducible Factor 1α in Tongue Sole, <I>Cynoglossus Semilaevis</I> (Actinopterygii: Pleuronectiformes: Cynoglossidae), Subjected to Acute Hypoxia

Molecular Cloning and Expression Analysis of Hypoxia Inducible Factor 1α in Tongue Sole, <I>Cynoglossus Semilaevis</I> (Actinopterygii: Pleuronectiformes: Cynoglossidae), Subjected to Acute Hypoxia

The effects of acute and chronic hypoxia on cortisol, glucose and lactate concentrations in different populations of three-spined stickleback

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

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