Acute and 28-Day Repeated Dose Oral Toxicity ofBauhinia variegata(Caesalpiniaceae) Stem Bark Extract

Abstract

Toxicity of aqueous extract (AE) of stem bark of Bauhinia variegata was evaluated. In the acute toxicity study, Sprague-Dawley rats were treated orally with AE (2,000 or 5,000 mg.kg −1 b.w). The animal behavior and mortality were observed periodically for up to 14 d. In the repeated dose toxicity study, the AE was administered daily at doses of 1,000 or 2,000 mg.kg −1 b.w, for 28 d. Behavior and mortality of animals were observed during the test period and hematological, biochemical parameters and histopathological examinations were carried out. In acute toxicity study, AE did not produce any mortality, changes in behavior, or any other physiological activities in rats at all selected doses. In repeated dose toxicity study, the monocytes and lymphocytes decreased at high doses. AE did not affect the biochemical parameters (except glucose) and the organs' weight. Histopathology studies revealed no changes in cellular structure of the organs studied. The AE of B. variegata had maximally tolerated dose (MTD) of 5,000 mg.kg −1 in acute toxicity study and lowest observed adverse effect level of 1,000 mg.kg −1 in repeated-dose toxicity study in rats.

The Effect of Bauhinia Variegata Ethanolic Extract on Monosodium Iodoacetate-Induced Osteoarthritis in Male Albino Rats

“…The present result demonstrated that all used BV extract doses (10, 100, 1000, 1600, 2900, and 5000 mg extract/kg body weight) didn't induce any mortality cases in male rats. Moreover, no changes, in general appearance, occurred during the observation period indicating that BV was well-tolerated (orally) in rats up to 5000 mg/kg body weight, this came in agreement with Makadiya et al [29] . An injection of MIA into a rodent's knee joint is a widely used experimental model to study OA [30] .…”

In Silico and In Vivo Toxicological Evaluation of Paeonol

“…Biochemical parameters like plasma total protein, creatinine, albumin, bilirubin, BUN, ALT, AST, ALP, TG, TC, uric acid levels were estimated by using biochemistry analyzer (Erba Chem 7, Germany). [35,36] Plasma samples were also estimated for potassium, sodium, and chloride levels.…”