Acupuncture plus Low-Frequency Electrical Stimulation (Acu-LFES) Attenuates Diabetic Myopathy by Enhancing Muscle Regeneration

Abstract

Mortality and morbidity are increased in patients with muscle atrophy resulting from catabolic diseases such as diabetes. At present there is no pharmacological treatment that successfully reverses muscle wasting from catabolic conditions. We hypothesized that acupuncture plus low frequency electric stimulation (Acu-LFES) would mimic the impact of exercise and prevent diabetes-induced muscle loss. Streptozotocin (STZ) was used to induce diabetes in mice. The mice were then treated with Acu-LFES for 15 minutes daily for 14 days. Acupuncture points were selected according to the WHO Standard Acupuncture Nomenclature guide. The needles were connected to an SDZ-II electronic acupuncture device delivering pulses at 20Hz and 1mA. Acu-LFES prevented soleus and EDL muscle weight loss and increased hind-limb muscle grip function in diabetic mice. Muscle regeneration capacity was significantly increased by Acu-LFES. The expression of Pax7, MyoD, myogenin and embryo myosin heavy chain (eMyHC) was significantly decreased in diabetic muscle vs. control muscle. The suppressed levels in diabetic muscle were reversed by Acu-LFES. The IGF-1 signaling pathway was also upregulated by Acu-LFES. Phosphorylation of Akt, mTOR and p70S6K were downregulated by diabetes leading to a decline in muscle mass, however, Acu-LFES countered the diabetes-induced decline. In addition, microRNA-1 and -206 were increased by Acu-LFES after 24 days of treatment. We conclude that Acu-LFES is effective in counteracting diabetes-induced skeletal muscle atrophy by increasing IGF-1 and its stimulation of muscle regeneration.

miRNA‐23a/27a attenuates muscle atrophy and renal fibrosis through muscle‐kidney crosstalk

“…Equal amounts of protein from TA muscle or whole kidney were used for western blot. 23,24 Protein bands were scanned and quantified by using the Li-cor Odyssey infrared scanning system (Li-COR Biosciences, Lincoin, Nebraska). Primary antibodies are listed in supporting information.…”

Secondary myopathy due to systemic diseases

“…Clinical manifestations vary depending on the underlying abnormality. The most frequent muscle manifestation in diabetes is painless muscle wasting (diabetic amyotrophy), which is either due to a diabetic plexus lesion or due to affection of satellite cells by the diabetes . A further frequent manifestation of diabetic myopathy is diabetic myonecrosis presenting as self‐limiting condition with acute onset of swelling and severe muscle pain .…”

“…The diagnosis is established by muscle MRI . Additionally, the expression level of Pax7, MyoD, myogenin, and fatal myosin‐heavy‐chain (MHC) is significantly decreased in diabetic myopathy .…”

Vázizomsérülés Kezelések Jelenlegi Módjainak Áttekintése

“…Kimutatták, hogy az elektromos akupunktúrás kezelés elnyomja a miosztatin expresszióját, ami a SC proliferációjához és a vázizom javulásához vezet [TAKAOKA et al, 2007]. Az akupunktúra és az alacsony frekvenciájú elektromos stimuláció (Acu-LFES) elősegítheti az izmok regenerálódását és megakadályozhatja az izomvesztést az izmok összehúzódásának stimulálása révén [SU et al, 2015]. Alkalmas lehet olyan súlyos betegségben szenvedő páciensek számára is, akik nem képesek testedzést végezni.…”